Effects of Partial Replacement of Corn with Different Levels of Defatted Rice Bran on Redox State and Cholesterol Metabolism of Suhuai Pigs

GAO Fangfang; LI Pinghua; ZHENG Weijiang; HUANG Ruihua; YAO Wen

doi:10.3969/j.issn.1006-267x.2020.02.018

2020 , Vol. 32 >Issue 2: 626 - 635

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.018

SWINE NUTRITION AND FEED

Effects of Partial Replacement of Corn with Different Levels of Defatted Rice Bran on Redox State and Cholesterol Metabolism of Suhuai Pigs

GAO Fangfang ,
LI Pinghua ,
ZHENG Weijiang ,
HUANG Ruihua ,
YAO Wen

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1. Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China;
2. Institute of Swine Science, Nanjing Agricultural University, Nanjing 210095, China;
3. Huai'an Academy of Nanjing Agricultural University, Huai'an 223005, China

Received date: 2019-07-29

Online published: 2020-02-21

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Abstract

The purpose of this study was to investigate the effects of partial replacement of corn with different levels of defatted rice bran on redox state and cholesterol metabolism of Suhuai castrated boars. A total of 21 healthy Suhuai castrated boars with similar body weight of (62.90±1.12) kg were randomly divided into control group (test group Ⅰ), test group Ⅱ and test group Ⅲ, with 7 replicates per group and 1 pig per replicate. Pigs in the test group Ⅰ were fed a basal diet, and those in the test group Ⅱ and test group Ⅲ were fed the basal diets replaced corn with 14% and 28% defatted rice bran, respectively. Soybean meal, bran and soybean oil, etc. were used to balance the digestible energy, crude protein and amino acid levels among the basal diet and the two experimental diets. The adaption period was 10 days, and the experiment period was 28 days. The results showed as follows:1) the serum malondialdehyde (MDA) content of Suhuai pigs in the test group Ⅱ and test group Ⅲ were significantly lower than that in the test group Ⅰ (P<0.05); compared with the test group Ⅰ and test group Ⅲ, the serum catalase (CAT) activity of Suhuai pigs in the test group Ⅱ was significantly higher (P<0.05); Suhuai pigs in the test group Ⅲ had the highest activity of CuZn superoxide dismutase (CuZn-SOD) in serum, which was significantly different from that in the test group Ⅰ (P<0.05); the liver glutathione (GSH) content and the ratio of glutathione to oxidized glutathione (GSH/GSSG) of Suhuai pigs in the test group Ⅲ were significantly higher than those in the test group Ⅰ (P<0.05). 2) Compared with the test group Ⅰ, the serum contents of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) of Suhuai pigs in the test group Ⅲ were significantly higher (P<0.05); there was no significant difference in the contents of liver TC and triglyceride (TG) among all groups (P>0.05). 3) Diets with defatted rice bran partially replacement of corn had no significant effects on the mRNA expression levels of cholesterol-7alpha-hydroxylase 1 (CYP7α1) and cholesterol-27alpha-hydroxylase 1 (CYP27α1) in the liver of Suhuai pigs (P>0.05); the mRNA expression levels of 3-hydroxy-3-mothylglutaryl coenzyme A reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP-2) and scaveger receptor class B type I (SR-BI) in the liver of Suhuai pigs in the test group Ⅱ and the test group Ⅲ were significantly lower than those in the test group Ⅰ (P<0.05). In addition, Suhuai pigs in the test group Ⅲ had the lowest low density lipoprotein receptor (LDLR) mRNA expression level in liver, which was significantly different from that in the test group Ⅰ (P<0.05). 4) Correlation analysis between serum contents of TC and LDL-C with oxidation marker and antioxidant enzymes in serum and liver of Suhuai pigs showed that serum TC content in the same individual was positively correlated with liver GSH content and GSH/GSSG (P<0.05). There was an extremely significant positive correlation between serum LDL-C content and liver GSH content as well as GSH/GSSG (P<0.01). In summary, under the conditions of this experiment, partial replacement of corn with different levels of defatted rice bran in the diet can improve the antioxidant capacity, and affect the cholesterol metabolism in serum and liver, and cholesterol-related gene expression in liver of Suhuai pigs.

Key words： defatted rice bran; Suhuai pigs; oxidative biomarker; antioxidant enzymes; cholesterol metabolism

Cite this article

GAO Fangfang , LI Pinghua , ZHENG Weijiang , HUANG Ruihua , YAO Wen . Effects of Partial Replacement of Corn with Different Levels of Defatted Rice Bran on Redox State and Cholesterol Metabolism of Suhuai Pigs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 626 -635 . DOI: 10.3969/j.issn.1006-267x.2020.02.018

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