SWINE NUTRITION AND FEED

Effects of Dietary L-Arginine on Liver Redox Status and Mitochondrial Function of Suckling Piglets with Low Birth Weight

  • ZHANG Hao ,
  • CHEN Daiwen ,
  • YU Bing ,
  • HE Jun ,
  • YU Jie ,
  • MAO Xiangbing ,
  • LUO Yuheng ,
  • HUANG Zhiqing ,
  • LUO Junqiu ,
  • ZHENG Ping
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  • Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2019-07-25

  Online published: 2020-02-21

Abstract

This study investigated the effects of L-arginine (Arg) on liver redox balance status and mitochondrial function of suckling piglets with low birth weight (LBW) aiming to explore the possible mechanism of Arg, which improved the growth and development of LBW suckling piglets. Piglets from primary sows with similar body condition, delivery period and parity were selected, and at 4 days of age, ten normal birth weight[NBW, body weight of (2.07±0.10) kg] piglets and twenty LBW[body weight of (1.16±0.08) kg] piglets were selected and divided into 3 groups with 10 replicates per group and 1 pig per replicate according to the similar body weight and consistent proportion of males and females. NBW piglets were fed a basal diet (a kind of artificial milk, NBW group), and LBW piglets were fed the basal diet (LBW group) or the basal diet supplemented with 1% Arg (LBW+Arg group). The experiment lasted for 21 days. At day 22, all piglets were slaughtered, then serum and liver samples were collected and growth performance, redox status, and mitochondrial function were determined. The results showed as follows:1) compared with NBW piglets, the final weight, average daily gain (ADG) and average daily dry matter intake (ADMI) of LBW piglets were significantly decreased (P<0.05), the liver catalase (CAT) activity and ATP content were also significantly reduced (P<0.05), the liver cyclooxygenase (COX)Ⅰ mRNA expression level was significantly down-regulated (P<0.05), and the liver optic atrophy protein 1 (OPA1) mRNA expression level was tended to be down-regulated (P=0.089). 2) Dietary Arg significantly improved the final weight, ADG and ADMI (P<0.05), significantly enhanced serum CAT activity and liver activity of CAT, superoxide dismutase (SOD) and glutathion peroxidase (GPx) (P<0.05), significantly increased liver ATP content (P<0.05), significantly up-regulated the mRNA expression levels of liver GPx1 and mitofusin 1 (Mfn1) (P<0.05), and tended to up-regulate the mRNA expression levels of liver CAT (P=0.056), COXⅣ (P=0.063) and OPA1 (P=0.087). In summary, LBW piglets exhibit lower liver antioxidant capacity and blocked mitochondrial function, which leads to impaired growth and development of piglets; while 1% Arg supplementation promotes growth performance through enhancing antioxidant capacity and improving mitochondrial function in liver of LBW piglets.

Cite this article

ZHANG Hao , CHEN Daiwen , YU Bing , HE Jun , YU Jie , MAO Xiangbing , LUO Yuheng , HUANG Zhiqing , LUO Junqiu , ZHENG Ping . Effects of Dietary L-Arginine on Liver Redox Status and Mitochondrial Function of Suckling Piglets with Low Birth Weight[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 654 -663 . DOI: 10.3969/j.issn.1006-267x.2020.02.021

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