Effects of Bacteriophage on Growth Performance, Intestinal pH, Volatile Fatty Acid Contents and Disaccharase Activity of Weaned Piglets

ZENG Yongdi; WANG Zirui; ZOU Tiande; ZHENG Liuzhen; LI Shuo; YOU Jinming

doi:10.3969/j.issn.1006-267x.2020.02.024

2020 , Vol. 32 >Issue 2: 682 - 690

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.024

SWINE NUTRITION AND FEED

Effects of Bacteriophage on Growth Performance, Intestinal pH, Volatile Fatty Acid Contents and Disaccharase Activity of Weaned Piglets

ZENG Yongdi ,
WANG Zirui ,
ZOU Tiande ,
ZHENG Liuzhen ,
LI Shuo ,
YOU Jinming

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Jiangxi Province Key Innovation Center of High-Quality and Safe Livestock Production, Jiangxi Province Key Laboratory of Animal Nutrition, Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2019-08-13

Online published: 2020-02-21

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Abstract

The purpose of this study was to investigate the effects of bacteriophage on growth performance, intestinal pH, volatile fatty acid contents and disaccharase activity of weaned piglets. A total of 120 piglets weaned at 25 days of age with the similar genetic background, healthy, similar parity and initial body weight of (7.35±0.03) kg were assigned randomly to 4 groups with 5 replicates per group and 6 piglets per replicate. Piglets in those 4 groups fed a basal diet containing antibiotics (50 mg/kg quinocetone and 25 mg/kg aureomycin) or a basal diet containing 200, 400 and 600 mg/kg bacteriophage, respectively. After 21 days, intestinal samples were collected to determine the pH, volatile fatty acids contents and digestive enzyme activities of intestinal contents. The results showed as follows:1) compared with the control group, the addition of 400 and 600 mg/kg bacteriophage in the diet significantly increased the weight gain of piglets in whole period (P<0.05). With the increase of bacteriophage supplemental levels in the diet, the weight gain of piglets presented a significantly linear and quadratic curve (P=0.001 and P=0.003). Moreover, diarrhea index of piglets in the 400 and 600 mg/kg bacteriophage groups was significantly lower than that in the control group and 200 mg/kg bacteriophage group (P<0.05). With the increase of bacteriophage supplemental levels, diarrhea index of piglets showed a significant linear and quadratic relationship (P=0.024 and P=0.015). 2) Compared with the control group, feeding bacteriophage at 400 mg/kg significantly reduced pH in ileum of piglets (P<0.05). 3) Bacteriophage addition of 400 and 600 mg/kg in the diet significantly increased the contents of acetic acid, butyric acid and total acid in the caecum compared with the control group (P<0.05), and the relationship between acetic acid (P<0.01 and P<0.01), butyric acid (P=0.017 and P=0.014) and total acid (P<0.01 and P<0.01) in the caecum showed significant linear and quadratic curves. The content of valeric acid in the 600 mg/kg bacteriophage group was higher than that in other groups (P<0.05), and the content of propionic acid was significantly higher than that in the 200 mg/kg bacteriophage group (P<0.05). Compared with the control group, the addition of bacteriophage of 400 and 600 mg/kg in the diet significantly increased the acetic acid and total acid contents in the colon of piglets (P<0.05). As the bacteriophage supplemental levels in the diet increased, the acetic acid content in the colon showed a significant quadratic curve relationship (P=0.008). The colonic butyric acid content of piglets in the 400 mg/kg bacteriophage group was significantly higher than that in the 200 and 600 mg/kg bacteriophage groups (P<0.05). 4) Compared with the control group, the duodenal sucrase and lactase activities of piglets in the 400 mg/kg bacteriophage group significantly increased (P<0.05). The activity of lactase in the duodenal mucosa increased significantly and then decreased (P=0.029), and the activity of galectin maltase significantly increased (P<0.05). In conclusion, results show that dietary bacteriophage supplementation can improve piglet growth performance and reduce diarrhea index, and improve intestinal digestive-absorption function through increasing intestinal short-chain fatty acid contents and digestive enzyme activity in piglets.

Key words： bacteriophage; weaned piglets; growth performance; short-chain fatty acids; disaccharase activity

Cite this article

ZENG Yongdi , WANG Zirui , ZOU Tiande , ZHENG Liuzhen , LI Shuo , YOU Jinming . Effects of Bacteriophage on Growth Performance, Intestinal pH, Volatile Fatty Acid Contents and Disaccharase Activity of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 682 -690 . DOI: 10.3969/j.issn.1006-267x.2020.02.024

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