RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Honeybee Peptide on Growth Performance and Rumen Microflora of Fattening Hu Sheep

  • ZHANG Zhian ,
  • LI Shiyi ,
  • WU Gang ,
  • LI Fadi ,
  • LI Fei
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  • 1. State Key Laboratory of Grassland Agro-Ecosystem, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730030, China;
    2. Gansu Olin Bell Biotechnology Group Limited Company, Zhangye 734000, China;
    3. Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China

Received date: 2019-07-31

  Online published: 2020-02-21

Abstract

This study was conducted to investigate the effects of honeybee peptide on growth performance and rumen microflora of fattening Hu sheep. A total of 180 healthy Hu male lambs with similarly body weight were selected and randomly divided into 5 groups with 6 replicates in each group and 6 sheep in each replicate. Sheep in the control group were fed a basal diet, and others in experimental groups were fed basal diets supplemented with 100, 200, 400 and 800 g/t honeybee peptide, respectively. The pre-experimental period lasted for 14 days, and the experimental period lasted for 63 days. The results showed as follows:1) the body weight on day 42 and body weight on day 63 of 400 and 800 g/t groups were significantly higher than those of the control group (P<0.05), the average daily gain of 200, 400 and 800 g/t groups was significantly higher than that of control group and 100 g/t group (P<0.05), and the ratio of feed to gain of 400 g/t group was significantly lower than that of the control group (P<0.05). 2) There was no significant difference in total volatile fatty acids (TVFA) content among all groups (P>0.05). The rumen fluid acetate proportion of 400 g/t group was significantly higher than that of the control group (P<0.05), and the propionate proportion was significantly lower than that of the control group (P<0.05). The rumen fluid acetate/propionate of 400 g/t group was significantly higher than that of the control group (P<0.05). 3) The rumen Selenomonas ruminantium number of 100, 200 and 400 g/t groups was significantly higher than that of the control group (P<0.05), the rumen Prevotella brevis number of 200 g/t group was significantly higher than that of the control group (P<0.05), the rumen Fibrobacter succinogenes number of 400 and 800 g/t groups was significantly lower than that of the control group and 100 g/t group (P<0.05), and the rumen Ruminobacter amylophilus number of 200 and 800 g/t groups was significantly higher than that of the control group and 100 g/t group (P<0.05). 4) The rumen Firmicutes abundance of 400 and 800 g/t groups was significantly higher than that of 200 g/t group (P<0.05), the rumen Butyrivibrio_2 abundance of 800 g/t group was significantly higher than that of the control group and 200 g/t group (P<0.05), the rumen Lachnospiraceae_NK3A20 abundance of 800 g/t group was significantly higher than that of other groups (P<0.05), and the rumen Ruminococcus_2 abundance of 400 g/t group was significantly higher than that of the control group and 200 g/t group (P<0.05). In summary, the addition of honeybee peptide in the diet of fattening Hu sheep is beneficial to the improvement of growth performance, and can regulate the rumen microflora and fermentation mode, which can be used as an ideal antibiotic substitute in actual production.

Cite this article

ZHANG Zhian , LI Shiyi , WU Gang , LI Fadi , LI Fei . Effects of Honeybee Peptide on Growth Performance and Rumen Microflora of Fattening Hu Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 756 -764 . DOI: 10.3969/j.issn.1006-267x.2020.02.031

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