AQUATIC ANIMAL NUTRITION AND FEED

Requirement of Lysine in Juvenile Tiger Puffer (Takifugu rubripes)

  • ZHANG Qinggong ,
  • WANG Jianxue ,
  • WEI Yuliang ,
  • XU Houguo ,
  • LIANG Mengqing
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  • 1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
    3. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China

Received date: 2019-08-27

  Online published: 2020-02-21

Abstract

The aim of this study was to investigate dietary lysine requirement for tiger puffer Takifugu rubripes. Six isonitrogenous and isoenergetic semi-purified diets were formulated to contain graded lysine levels (1.65%, 2.58%, 3.18%, 4.01%, 4.80% and 5.52%). Juvenile tiger puffer[initial body weight (13.83±0.63) g] were randomly assigned to 18 tanks (30 fish per tank and 3 replicate tanks per group) in indoor flow-through and aerated aquaria for 67 days. The results showed as follows:the specific growth rate, feed efficiency and protein deposition rate firstly increased and then decreased with the increase of dietary lysine level, and reached the highest value when experimental diets contained 4.80% lysine, which was significantly higher than that in 1.65% lysine group (P<0.05). The whole body crude lipid content, hepatosomatic index and viscerosomatic index in the 1.65% group was significantly higher than that in other groups (P<0.05). Glutamic oxaloacetic transaminase activity in liver and serum increased first and then decreased with the increase of dietary lysine level. Serum high density lipoprotein cholesterol content increased with the increase of dietary lysine level, which was significantly higher in the 4.80% and 5.52% groups than that in 1.65% group (P<0.05). Second-order polynomial regression analysis on the basis of specific growth rate (SGR) indicates that the optimum dietary lysine requirements of tiger puffer is estimated to be 3.83% of diet (7.66% of dietary protein).

Cite this article

ZHANG Qinggong , WANG Jianxue , WEI Yuliang , XU Houguo , LIANG Mengqing . Requirement of Lysine in Juvenile Tiger Puffer (Takifugu rubripes)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 847 -855 . DOI: 10.3969/j.issn.1006-267x.2020.02.040

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