Breed Difference of Porcine CRTC3 Expression and Its Regulation by Forskolin

LIU Jiaqi; LIU Jiali; NONG Qiuyun; SHAN Tizhong

doi:10.3969/j.issn.1006-267x.2020.02.042

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 2: 870 - 880

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.042

MOLECULAR AND CELLULAR NUTRITION

Breed Difference of Porcine CRTC3 Expression and Its Regulation by Forskolin

LIU Jiaqi ,
LIU Jiali ,
NONG Qiuyun ,
SHAN Tizhong

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Key Laboratory of Animal Molecular Nutrition, Ministry of Education, College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2019-08-21

Online published: 2020-02-21

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Abstract

CRTC3 is a key gene in glucose and lipid metabolism pathway. In this study, we aimed to study the expression pattern of porcine CRTC3 in muscle and adipose tissues of different breeds, and the effects of forskolin treatment on expression of CRTC3 gene and differentiation in porcine subcutaneous preadipocytes. Five Laiwu pigs and five Duroc×Landrace×Yorkshire (DLY) pigs were selected to detect the mRNA and protein expression levels of CRTC3 and lipid metabolism related genes in muscle and adipose tissues. Before differentiation, the porcine subcutaneous preadipocytes were separated from 3-day-old DLY piglets. The above medium was replaced by DMEM containing MDI induction 4 days. Then the medium was replaced by DMEM containing insulin until day 8. 10 μmol/L forskolin was added at the 1^st day of differentiation and induction in forskolin group, and the dimethyl sulfoxide (DMSO) with the same concentration was added in control group. The results showed as follows:compared with DLY pigs, Laiwu pig had a higher CRTC3 protein expression level in longissimus dorsi muscle and psoas major muscle. The mRNA expression levels of CRTC3 and fat deposition related genes peroxisome proliferators activated receptor γ (PPARγ), fatty acid binding protein 4 (FABP4), CCAAT/enhancer binding protein α (C/EBPα), perilipin (PLIN) and leptin (LEP) in subcutaneous and visceral adipose tissues of Laiwu pigs were significantly higher than those of DLY pigs (P<0.05 or P<0.01). However, the mRNA expression levels of adipose browning related genes NF-E2 related factor 1 (NRF1), PPARγ coactivator 1α (PGC-1α), PRD1-BF1-RIZ1 homologous domain containing 16 (PRDM16), uncoupling protein 2 (UCP2) and uncoupling protein 3(UCP3) in subcutaneous and visceral adipose tissues of Laiwu pigs were significantly or extremely significantly lower than those of DLY pigs (P<0.05 or P<0.01). Furthermore, the mRNA expression levels of CRTC3, adipose deposition related genes and adipose browning related genes in porcine subcutaneous preadipocytes were extremely significantly increased after differentiation (P<0.01). Treatment with 10 μmol/L forskolin inhibited the differentiation of porcine subcutaneous preadipocytes, upregulated the mRNA expression levels of cAMP-response element binding protein (CREB) and adipose browning related genes, promoted the entry of CRTC3 into the nucleus. Moreover, it reduced the mRNA expression levels of CRTC3 (P<0.01), as well as adipose deposition related genes (P<0.01). Therefore, CRTC3 is a adipose deposition related gene for selecting bioactive or nutrient factors, which should be applicable to regulation of fat deposition.

Key words： pigs; CRTC3; fat deposition; differentiation; forskolin

Cite this article

LIU Jiaqi , LIU Jiali , NONG Qiuyun , SHAN Tizhong . Breed Difference of Porcine CRTC3 Expression and Its Regulation by Forskolin[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 870 -880 . DOI: 10.3969/j.issn.1006-267x.2020.02.042

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