Effects of Apidaecin on Rumen Fermentation Parameters and Methane Emissions by in Vitro Fermentation

LI Shiyi; GUO Tongqing; LIU Xin; WU Gang; LI Fei; NIU Xiaolin; ZHANG Zhian; LI Fadi

doi:10.3969/j.issn.1006-267x.2020.02.050

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 2: 941 - 947

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.02.050

SHORT COMMUNICATION

Effects of Apidaecin on Rumen Fermentation Parameters and Methane Emissions by in Vitro Fermentation

LI Shiyi ,
GUO Tongqing ,
LIU Xin ,
WU Gang ,
LI Fei ,
NIU Xiaolin ,
ZHANG Zhian ,
LI Fadi

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1. State Key Laboratory of Grassland Agro-Ecosystem, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
2. Gansu Olin Bell Biotechnology Group Limited Company, Zhangye 734000, China;
3. Engineering Laboratory of Mutton Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China

Received date: 2019-08-09

Online published: 2020-02-21

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Abstract

The purpose of this experiment was to study the effects of apidaecin on rumen fermentation parameters and methane emissions by in vitro fermentation. Four healthy similar weight[(40±3) kg] alpine Merino sheep were selected as rumen liquid donors. A single factor design was used in the experiment. Three treatments were added with apidaecin at 0, 0.75 and 1.50 mg per kg of dry matter, respectively. Each treatment was repeated seven times. Fermentation was terminated in ice water for 24 h. Gas production and fermentation broth were collected. The pH of rumen fermentation broth was measured, and the effects of apidaecin on gas production, volatile fatty acid contents and some microorganisms number in fermentation broth were also measured. The results showed that fermented in vitro for 24 h, compared with no apidaecin addition, adding 0.75 mg apidaecin could significantly increase the percentage of acetic acid and acetic-to-propionic acid ratio in rumen (P<0.05), significantly reduce the percentage of propionic acid (P<0.05), and significantly increase methane production (P<0.05). Meanwhile, there was a tendency to increase rumen pH (P=0.080) and the number of Prevotella (P=0.078). In summary, under the condition of 24 h fermentation in vitro, apidaecin has a certain effect on the rumen fermentation of sheep, which can regulate the rumen fermentation mode of sheep.

Key words： in vitro fermentation; apidaecin; rumen fermentation; methane

Cite this article

LI Shiyi , GUO Tongqing , LIU Xin , WU Gang , LI Fei , NIU Xiaolin , ZHANG Zhian , LI Fadi . Effects of Apidaecin on Rumen Fermentation Parameters and Methane Emissions by in Vitro Fermentation[J]. Chinese Journal of Animal Nutrition, 2020 , 32(2) : 941 -947 . DOI: 10.3969/j.issn.1006-267x.2020.02.050

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