This experiment was conducted to analyze the difference of nutritional quality between grass carp cultured in circulating flume and intensive culture pond. Twelve grass carp cultured in circulating flume[average body weight:(723.89±71.54) g, average body length:(340.86±8.88) mm] and intensive culture pond[average body weight:(680.35±155.13) g, average body length:(334.00±21.40) mm]and fed the same feed source were selected, respectively, which were used to analyze morphological indices, and muscle texture characteristics, color, shearing force, the contents of common nutrients, amino acids, fatty acids and mineral elements. The results were as follows:1) the hardness, gumminess, chewiness, resilience and shearing force of grass carp cultured in circulating flume were significantly higher than those of grass carp cultured in pond intensive (P<0.05). 2) The muscle moisture content of grass carp cultured in circulating flume was significantly lower than that of grass carp cultured in pond intensive grass carp (P<0.05), the muscle crude fat was lower than that of grass carp cultured in pond intensive grass carp, and the muscle crude protein and ash contents were higher than those of grass carp cultured in pond intensive grass carp, but no significant difference in muscle crude fat, crude protein and ash contents (P>0.05). 3) The total amount of amino acids(TAA), the total amount of essential amino acids (TEAA) and the total amount of delicious amino acids (TDAA) in muscle of grass carp cultured in circulating flume were higher than those of grass carp cultured in pond intensive grass carp, but the differences were not significant (P>0.05). The TAA of grass carp cultured in circulating flume and intensive culture pond was 77.11% and 76.61%, and the TEAA and the TDAA accounted for were 40.87% and 40.43%, 46.10% and 46.06%, respectively, of the TAA. 4) The proportion of unsaturated fatty acids (UFA) in total fatty acids (69.68%) in muscle of grass carp cultured in circulating flume was higher than that of grass carp cultured in intensive culture pond (68.44%), but no significant difference (P>0.05). The proportion of saturated fatty acids (SFA) in total fatty acids in muscle of grass carp cultured in circulating flume and intensive culture pond was 21.78% and 18.58%, respectively, and the difference was significant (P<0.05). Eicosapentaenoic acid (EPA) was not detected and the content of docosahexaenoic acid (DHA) was 0.44% in muscle of grass carp cultured in circulating flume, and the contents of EPA and DHA in muscle of grass carp cultured in intensive culture pond were 0.10% and 0.55%, respectively. 5) The contents of potassium (K), magnesium (Mg), iron (Fe) and copper (Cu) in muscle of grass carp cultured in circulating flume were significantly higher than those of grass carp cultured in intensive culture pond (P<0.05), while the contents of sodium (Na) and zinc (Zn) in muscle were significantly lower than those of grass carp cultured in intensive culture pond (P<0.05). To sum up, the nutritional quality of grass carp cultured in circulating flume is slightly better than that of grass carp cultured in intensive culture pond.