Effects of Fenugreek Residue and Extract on Growth Performance, Antioxidant Function and Serum Biochemical Indices of Weaned Piglets

  • XIA Xin ,
  • YU Manrong ,
  • HUANG Hui ,
  • FU Xiaoqin ,
  • ZHOU Yingjun ,
  • XIAO Dingfu
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Geneham Pharmaceutical Co., Ltd., Changsha 410014, China;
    3. Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China

Received date: 2019-09-09

  Online published: 2020-03-13

Abstract

This experiment was conducted to study the effects of fenugreek residue and extract on growth performance, antioxidant function and serum biochemical indices of weaned piglets. A total of 160 healthy 21-day-old weaned piglets with similar body weight were randomly divided into 4 groups with 5 replicates per group and 8 pigs per replicate. Pigs in the control group were fed a basal diet, and others in experimental groups (groups Ⅰ, Ⅱ and Ⅲ) were fed basal diets supplemented with 5% fenugreek residue, 5% fenugreek residue complex (contained 0.05% fenugreek extract) and 5% fenugreek residue complex (contained 0.10% fenugreek extract) respectively. The pre-feeding period was 3 days and the formal period was 21 days. The results showed as follows:1) the average daily gain (ADG) of weaned piglets of groups Ⅱ and Ⅲ had no significant difference compared with the control group (P>0.05); the average daily feed intake(ADFI) of weaned piglets of groups Ⅱ and Ⅲ was significantly higher than that of the control group (P<0.05), which increased by 9.22% and 9.19%, respectively; the feed to gain ratio (F/G) of weaned piglets of experimental groups was significantly higher than that of the control group (P<0.05); and there was no significant difference in diarrhea rate of weaned piglets among all groups (P>0.05). 2) The total antioxidant capacity(T-AOC) and total superoxide dismutase (T-SOD) and catalase(CAT) activities in serum of weaned piglets of groups Ⅱ and Ⅲ were significantly higher than those of the control group (P<0.05), the serum malondialdehyde (MDA) content of weaned piglets of groups Ⅰ, Ⅱ and Ⅲ was significantly lower than that of the control group (P<0.05). There was no significant difference in serum glutathione peroxidase (GSH-Px) activity of weaned piglets among all groups (P>0.05). 3) The serum creatinine (CREA) content of weaned piglets of groups Ⅱ and Ⅲ was significantly lower than that of the control group (P<0.05), the serum glucose (GLU) content of weaned piglets of group Ⅲ was significantly lower than that of the control group (P<0.05), and the serum urea nitrogen (UN) content of weaned piglets of groups Ⅱ and Ⅲ was significantly higher than that of the control group (P<0.05). There were no significant differences in glutamic oxaloacetic transaminase (AST), alanine aminotransferase (ALT) activities and total cholesterol (TC), total protein (TP), albumin (ALB), globulin (GLO) and triglyceride (TG) contents in serum among all groups (P>0.05). In conclusion, dietary supplemented with 5% fenugreek residue complex (contained 0.05% and 0.10 fenugreek extract, respectively) has an attractive effect on weaned piglets, which can improve the antioxidant function, and reduce blood sugar level and protect kidneys, and the effects of supplemented with 0.10% fenugreek extract is better.

Cite this article

XIA Xin , YU Manrong , HUANG Hui , FU Xiaoqin , ZHOU Yingjun , XIAO Dingfu . Effects of Fenugreek Residue and Extract on Growth Performance, Antioxidant Function and Serum Biochemical Indices of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1118 -1126 . DOI: 10.3969/j.issn.1006-267x.2020.03.018

References

[1] WANI S A,KUMAR P.Fenugreek:a review on its nutraceutical properties and utilization in various food products[J].Journal of the Saudi Society of Agricultural Sciences,2018,17(2):97-106.  
[2] ZANDI P,BASU S K,KKATIBANI L B,et al.Fenugreek (Trigonella foenum-graecum L.) seed:a review of physiological and biochemical properties and their genetic improvement[J].Acta Physiologiae Plantarum,2015,37(1):1714.
[3] 陈君,丁万隆,陈震,等.不同种源胡芦巴生长发育特性初步观察[J].中国中药杂志,2001,26(4):237-240.
[4] RAYYAN S,FOSSEN T,ANDERSEN M Ø.Flavone C-glycosides from seeds of fenugreek,Trigonella foenum-graecum L.[J].Journal of Agricultural and Food Chemistry,2010,58(12):7211-7217.  
[5] PARI L,MONISHA P,JALALUDEEN A M.Beneficial role of diosgenin on oxidative stress in aorta of streptozotocin induced diabetic rats[J].European Journal of Pharmacology,2012,691(1/2/3):143-150.
[6] 卢芙蓉,沈霖,秦铀,等.葫芦巴总皂苷联合磺脲类降糖药治疗2型糖尿病36例临床观察[J].中国中药杂志,2008,33(2):184-187.
[7] 卢芙蓉,杨胜兰,沈霖,等.葫芦巴提取物对营养性肥胖大鼠糖脂的调节效应[J].世界华人消化杂质,2012,20(30):2902-2906.
[8] 尚明英,王旋,蔡少青,等.胡芦巴油脂成分的分析测定[J].中草药,1999,30(4):256-257.
[9] KUMAR H,DUBEY R C,MAHESHWARI D K.Effect of plant growth promoting rhizobia on seed germination,growth promotion and suppression of Fusarium wilt of fenugreek (Trigonella foenum-graecum L.)[J].Crop Protection,2011,30(11):1396-1403.  
[10] HALLIWELL B,WHITEMAN M.Measuring reactive species and oxidative damage in vivo and in cell culture:how should you do it and what do the results mean?[J].British Journal of Pharmacology,2004,142(2):231-255.  
[11] 孙国栋,李新霞,李琳琳,等.胡芦巴提取物化学成分含量测定及体外抗氧化研究[J].新疆医科大学学报,2013,36(6):772-776.
[12] XIAO D F,YUAN D X,TAN B H,et al.The role of Nrf2 signaling pathway in Eucommia ulmoides flavones regulating oxidative stress in the intestine of piglets[J].Oxidative Medicine and Cellular Longevity,2019,2019:9719618.
[13] GUARDIOLA F A,BAHI A,BAKHROUF A,et al.Effects of dietary supplementation with fenugreek seeds,alone or in combination with probiotics,on gilthead seabream (Sparus aurata L.) skin mucosal immunity[J].Fish & Shellfish Immunology,2017,65:169-178.
[14] HERREEA T,NAVARRO D H J,FORNARI T,et al.Inhibitory effect of quinoa and fenugreek extracts on pancreatic lipase and α-amylase under in vitro traditional conditions or intestinal simulated conditions[J].Food Chemistry,2019,270:509.
[15] GLADE M J,MEGUID M M.A glance at telomeres,oxidative stress,antioxidants,and biological aging[J].Nutrition,2015,31(11/12):1447-1451.
[16] KIM M H,LEE E J,CHEON J M,et al.Antioxidant and hepatoprotective effects of fermented red ginseng against high fat diet-induced hyperlipidemia in rats[J].Laboratory Animal Research,2016,32(4):217-223.  
[17] KUMAR S,SHARMA U K,SHARMA A K,et al.Protective efficacy of Solanum xanthocarpum root extracts against free radical damage:phytochemical analysis and antioxidant effect[J].Cellular and Molecular Biology,2012,58(1):174-181.
[18] KHALILI M,ALAVI M,ESMAEIL-JAMAAT E,et al.Trigonelline mitigates lipopolysaccharide-induced learning and memory impairment in the rat due to its anti-oxidative and anti-inflammatory effect[J].International Immunopharmacology,2018,61:355-362.
[19] 王雅,赵萍,任海伟,等.微波辅助萃取葫芦巴茎叶挥发油工艺优化及抗氧化性研究[J].食品科学,2010,31(18):120-123.
[20] 吴婷,闫茂华,张娅,等.葫芦巴多糖酸解和酶解产物抗氧化及抗菌活性的比较研究[J].食品科学,2007,28(11):509-512.
[21] 陈帅,王慧竹,钟方丽,等.葫芦巴总黄酮提取工艺及其体外抗氧化活性[J].青岛科技大学学报(自然科学版),2015,36(6):628-634.
[22] KTARI N,FEKI A,TRABELSI I,et al.Structure,functional and antioxidant properties in Tunisian beef sausage of a novel polysaccharide from Trigonella foenum-graecum seeds[J].International Journal of Biological Macromolecules,2017,98:169-181.
[23] JOGLEKAR M,MANDAL M,PARALAKOTI M,et al.Comparative analysis of antioxidant and antibacterial properties of Aegle marmelos,Coriandrum sativum and Trigonella foenum graecum[J].Acta Biologica Indica,2012,1(1):105-108.
[24] TANKO Y,KABIRU A,ABDULRASAK A,et al.Effects of fermented ginger rhizome (Zingiber officinale) and Fenu Greek (Trigonella foenum-graceum) supplements on oxidative stress and lipid peroxidation biomarkers in poloxamer-407 induced-hyperlipidemic wistar rats[J].Nigerian Journal of Physiological Sciences,2017,32(2):137-143.
[25] GUARDIOLA F A,BAHI A,MESSINA C M,et al.Quality and antioxidant response of gilthead seabream (Sparus aurata L.) to dietary supplements of fenugreek (Trigonella foenum graecum) alone or combined with probiotic strains[J].Fish & Shellfish Immunology,2017,63:277-284.
[26] GUARDIOLA F A,BAHI A,JIMÉNEZ-MONREAL A M,et al.Dietary administration effects of fenugreek seeds on skin mucosal antioxidant and immunity status of gilthead seabream (Sparus aurata L.)[J].Fish & Shellfish Immunology,2018,75:357-364.
[27] 蔡旭滨,陈凌锋,檀新珠,等.太子参茎叶多糖对断奶仔猪生长性能和血清抗氧化指标、免疫指标及生化指标的影响[J].动物营养学报,2016,28(12):3867-3874.
[28] LIU H,JI H F,ZHANG D Y,et al.Effects of Lactobacillus brevis preparation on growth performance,fecal microflora and serum profile in weaned pigs[J].Livestock Science,2015,178:251-254.
[29] 周吉银,江明金,周世文,等.胡芦巴调脂有效成分及其作用机制研究概述[J].中药药理与临床,2014,30(3):157-160.
[30] JIANG W Y,SI L H,LI P D,et al.Serum metabonomics study on antidiabetic effects of fenugreek flavonoids in streptozotocin-induced rats[J].Journal of Chromatography B,2018,1092:466-472.
[31] MUKTHAMBA P,SRINIVASAN K.Hypolipidemic influence of dietary fenugreek (Trigonella foenum-graecum) seeds and garlic (Allium sativum) in experimental myocardial infarction[J].Food & Function,2015,6(9):3117-3125.  
[32] JAISWAL N,MAURYA C K,VENKATESWARLU K,et al.4-Hydroxyisoleucine stimulates glucose uptake by increasing surface GLUT4 level in skeletal muscle cells via phosphatidylinositol-3-kinase-dependent pathway[J].European Journal of Nutrition,2012,51(7):893-898.  
[33] 余海,吴萌,卢芙蓉,等.葫芦巴4-羟基异亮氨酸对高糖诱导小鼠3T3-L1脂肪细胞胰岛素抵抗的影响[J].中国中西医结合杂志,2013,33(10):1394-1399.
[34] 汪佳琦,姜文月,张琳,等.胡芦巴中皂苷、黄酮和多糖组分不同配伍对1型糖尿病大鼠的降血糖作用[J].吉林大学学报(医学版),2016,42(6):1081-1086.
[35] 刘正旭.竹酢粉对断奶仔猪生产性能、免疫机能及肠道微生物区系的影响[D].硕士学位论文.扬州:扬州大学,2016.
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