Difference Comparison of Liver Lipid Metabolism between Meishan and Duroc×Landrace×Yorkshire Newborn Piglets

  • ZHANG Yongsheng ,
  • YU Lihuai ,
  • MAO Junzhou ,
  • PENG Zhong ,
  • ZHONG Zhaoxin ,
  • WANG Tianlong ,
  • DONG Li
Expand
  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2019-09-17

  Online published: 2020-03-13

Abstract

This experiment aimed to compare the differences in liver lipid metabolism between newborn piglets of different breeds. Every six Meishan and Duroc×Landrace×Yorkshire (DLY) piglets with the same parity were selected without feeding breast milk and were slaughtered by internal carotid artery bleeding in 6 h after birth for taking samples. The results showed as follows:1) the serum triglyceride (TG) content of newborn Meishan piglets was extremely significantly higher than that of newborn DLY piglets (P<0.01). The serum contents of total cholesterol (TC) and low density lipoprotein (LDL) of the two breeds of newborn piglets were not significantly different (P>0.05). 2) The liver index of newborn DLY piglets was extremely significantly higher than that of newborn Meishan piglets (P<0.01), and the liver cell area and particle size of newborn DLY piglets were significantly higher than that of newborn Meishan piglets (P<0.05). 3) The contents of total TC, TG and high density lipoprotein cholesterol (HDL-C) in liver of newborn Meishan piglets were extremely significantly higher than that of newborn DLY piglets (P<0.01), and the liver lipoprotein lipase (LPL) activity of newborn Meishan piglets was significantly lower than that of newborn DLY piglets (P<0.05). 4) The liver LPL mRNA expression level of newborn Meishan piglets was extremely significantly lower than that of newborn DLY piglets (P<0.01), and the liver fatty acid synthase (FAS) mRNA expression level of newborn Meishan piglets was extremely significantly higher than that of newborn DLY piglets (P<0.01). In summary, the liver lipid anabolic function of the newborn Meishan piglets is stronger than that of newborn DLY piglets, which is related to the differences in liver lipid synthesis and catabolism gene expression between them.

Cite this article

ZHANG Yongsheng , YU Lihuai , MAO Junzhou , PENG Zhong , ZHONG Zhaoxin , WANG Tianlong , DONG Li . Difference Comparison of Liver Lipid Metabolism between Meishan and Duroc×Landrace×Yorkshire Newborn Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1143 -1150 . DOI: 10.3969/j.issn.1006-267x.2020.03.021

References

[1] 王艳.大白猪与梅山猪肝脏组织差异表达基因的分离以及基因功能的研究[D].博士学位论文.武汉:华中农业大学,2009.
[2] KIM N K,PARK H R,LEE H C,et al.Comparative studies of skeletal muscle proteome and transcriptome profilings between pig breeds[J].Mammalian Genome,2010,21(5/6):307-319.
[3] AYUSO M,FERNÁNDEZ A,NÚÑEZ Y,et al.Comparative analysis of muscle transcriptome between pig genotypes identifies genes and regulatory mechanisms associated to growth,fatness and metabolism[J].PLoS One,2015,10(12):e0145162.
[4] MARTÍNEZ-ÁLVARO M,PAUCAR Y,SATUÉ K,et al.Liver metabolism traits in two rabbit lines divergently selected for intramuscular fat[J].Animal,2018,12(6):1217-1223.  
[5] LIU Y Z,YANG X Q,JING X Y,et al.Transcriptomics analysis on excellent meat quality traits of skeletal muscles of the Chinese indigenous min pig compared with the large white breed[J].International Journal of Molecular Sciences,2018,19(1):21.
[6] 朱志刚.交互寄养对梅山与大白仔猪生长性能、消化酶活性、血液生化指标及脂肪代谢的影响[D].硕士学位论文.南京:南京农业大学,2012.
[7] LI C L,PAN Y C,MENG H.Polymorphism of the H-FABP,MC4R and ADD1 genes in the Meishan and four other pig populations in China[J].South African Journal of Animal Science,2006,36(1):1-6.
[8] MOUROT J,KOUBA M,BONNEAU M.Comparative study of in vitro lipogenesis in various adipose tissues in the growing Meishan pig:comparison with the large white pig (Sus domesticus)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1996,115(3):383-388.  
[9] CHEN P,BAAS T J,MABRY J W,et al.Genetic parameters and trends for litter traits in U.S. Yorkshire,Duroc,Hampshire,and Landrace pigs[J].Journal of Animal Science,2003,81(1):46-53.  
[10] CALABUIG-NAVARRO V,HAGHIAC M,MINIUM J,et al.Effect of maternal obesity on placental lipid metabolism[J].Endocrinology,2017,158(8):2543-2555.  
[11] 慈乐.母猪泌乳期日粮脂肪水平对子代脂肪酸组成及脂质代谢的影响[D].硕士学位论文.南京:南京农业大学,2014.
[12] 陶莲,冯文晓,王玉荣,等.不同方法青贮的玉米秸秆对肉羊屠宰性能、组织和器官发育及血清生化指标的影响[J].动物营养学报,2018,30(1):84-91.
[13] CAI D M,LIU H Y,HU Y,et al.Gestational betaine,liver metabolism,and epigenetics[M]//PATEL V,PREEDY V.Handbook of nutrition,diet,and epigenetics.Cham:Springer,2017:1-14.
[14] 李清宏.美系獭兔生长发育规律的研究[D].博士学位论文.晋中:山西农业大学,2013.
[15] YANG H S,LI F N,XIONG X,et al.Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue,skeletal muscle and liver of male Huanjiang mini-pigs[J].Molecular and Cellular Endocrinology,2013,365(1):44-51.  
[16] 王艳,林禹翔,康劲翮,等.玉竹多糖对高脂饲料喂养的小鼠脂代谢的影响[J].营养学报,2018,40(4):353-359.
[17] HUANG Q C,XU Z R,HAN X Y,et al.Changes in hormones,growth factor and lipid metabolism in finishing pigs fed betaine[J].Livestock Science,2006,105(1/2/3):78-85.
[18] SUNDRAM K,PERLMAN D,HAYES K C.Increasing the HDL level and the HDL/LDL ratio in human serum with fat blends:USA,5578334[P].1996.
[19] KIM J S,INGALE S L,LEE S H,et al.Impact of dietary fat sources and feeding level on adipose tissue fatty acids composition and lipid metabolism related gene expression in finisher pigs[J].Animal Feed Science and Technology,2014,196:60-67.
[20] 刘润,李建科,程玉江,等.石榴鞣花酸对高脂血仓鼠肝脏脂质合成与分解基因表达的影响[J].营养学报,2015,37(4):399-403.
[21] 明鹏飞,黄莹莹,邹苏萍,等.高脂血症奶牛乳腺组织中脂代谢相关生化指标和基因表达的变化[J].动物营养学报,2018,30(5):1807-1815.
[22] 王怡平,徐晨晨,罗海玲.动物肌内脂肪沉积相关候选基因的研究进展[J].动物营养学报,2017,29(5):1475-1480.
[23] ZHANG C,LUO J Q,YU B,et al.Effects of resveratrol on lipid metabolism in muscle and adipose tissues:a reevaluation in a pig model[J].Journal of Functional Foods,2015,14:590-595.
[24] SZKUP M,OWCZAREK A J,SCHNEIDER-MATYKA D,et al.Associations between the components of metabolic syndrome and the polymorphisms in the peroxisome proliferator-activated receptor gamma (PPAR-γ),the fat mass and obesity-associated (FTO),and the melanocortin-4 receptor (MC4R) genes[J].Aging,2018,10(1):72-82.  
[25] MCNEEL R L,DING S T,SMITH E O,et al.Effect of feed restriction on adipose tissue transcript concentrations in genetically lean and obese pigs[J].Journal of Animal Science,2000,78(4):934-942.  
[26] 付言峰,李碧侠,李兰,等.苏钟猪脂蛋白脂肪酶的组织表达谱及其生物信息学分析[J].畜牧兽医学报,2012,43(12):1871-1878.
[27] ZHANG C,LUO J Q,ZHENG P,et al.Differential expression of lipid metabolism-related genes and myosin heavy chain isoform genes in pig muscle tissue leading to different meat quality[J].Animal,2015,9(6):1073-1080.  
Outlines

/