Correlation between Protein Molecular Structure and Protein Nutritional Value or Ruminal Degradation Characteristics in Different Heat-Treated Cottonseeds

XU Wenbin; GAO Bolan; ZHU Quanchao; QI Danjie; GAO Yue; LI Haoran; SUN Manji

doi:10.3969/j.issn.1006-267x.2020.03.035

2020 , Vol. 32 >Issue 3: 1272 - 1283

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.035

Correlation between Protein Molecular Structure and Protein Nutritional Value or Ruminal Degradation Characteristics in Different Heat-Treated Cottonseeds

XU Wenbin ,
GAO Bolan ,
ZHU Quanchao ,
QI Danjie ,
GAO Yue ,
LI Haoran ,
SUN Manji

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College of Animal Science and Technology of Northeast Agricultural University, Harbin 150030, China

Received date: 2019-09-05

Online published: 2020-03-13

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Abstract

The objective of this experiment was to explore the effects of different heat treatments on nutritional value, rumen degradation characteristics, small intestinal digestibility and molecular structure of cottonseed protein, and to discuss whether there was a correlation between protein molecular structure and protein nutritional value, rumen degradation characteristics or small intestinal digestibility in cottonseed. Cottonseed was treated by dry heat (heating at 140, 150 and 160℃ for 1 h, respectively), microwave (heating at 700 W for 1, 2 and 3 min, respectively) and γ-irradiation (doses were 30, 45 and 60 kGy, respectively), respectively. The protein nutritional value, rumen degradation characteristics and small intestinal digestibility of different heat-treated cottonseeds were determined by traditional chemical analysis combined with Cornell net carbohydrate-net protein system (CNCPS), nylon bag method and modified three-step in vitro method. Fourier transform infrared spectroscopy (FTIR) technology was used to analyze the protein molecular structure of different heat-reated cottonseeds, and then to analyze the correlation relationships between protein molecular structure and protein nutritional value, rumen degradation characteristics or small intestinal digestibility in cottonseed. The results showed as follows:1) different heat treatments had no significant effects on protein composition and CNCPS protein components of cottonseed (P>0.05). 2) Different heat treatments extremely significantly affected the rumen soluble part, rumen degradable part, rumen undegradable part, rumen protein effective degradation rate and the contents of small intestinal degradable protein and total degradable protein (P<0.01). With heat treatment time or strength increasing, the rumen protein effective degradation rate and total degradable protein content and the small intestinal digestibility of rumen undegradable protein were gradually decreased, while the small intestinal degradable protein content was gradually increased. 3) Different heat treatments extremely significantly affected amide Ⅰ band peak height and peak area, amide Ⅱ band peak height and peak area, α-helix peak height and α-helix and β-sheet peak height ratio (P<0.01). Except microwave heating at 3 min, other heat treatments all significantly increased the amide Ⅰ band peak height and peak area, amide Ⅱ band peak height and peak area (P<0.05), and 45 and 60 kGy γ-irradiation also significantly increased the α-helix peak height and α-helix and β-sheet peak height ratio (P<0.05). 4) The molecular structure of cottonseed protein was correlated with its nutritional value, rumen degradation characteristics and small intestinal digestibility, and regression equations could be established. The R² of regression equations for neutral detergent insoluble protein, soluble protein, rapidly degradable true protein, intermediately degradable true protein and slowly degradable true protein all exceed 0.9, but the R² of part regression equations lowed 0.6. In summary, three heat treatments have effects on the rumen degradation characteristics, small intestinal digestibility and molecular structure of cottonseed protein. Heat treatment can increase the contents of ruminal protein and small intestinal digestible protein, and the effect is the best when the irradiation dose is 60 kGy. The molecular structure of cottonseed protein has a correlation with its nutritional value, rumen degradation characteristics and small intestinal digestibility, and can establish regression equations. However, the R² of some regression equations is less than 0.6, still need further research.

Key words： heat treatment; cottonseed; FTIR; protein molecular structure; nutritional value; correlation

Cite this article

XU Wenbin , GAO Bolan , ZHU Quanchao , QI Danjie , GAO Yue , LI Haoran , SUN Manji . Correlation between Protein Molecular Structure and Protein Nutritional Value or Ruminal Degradation Characteristics in Different Heat-Treated Cottonseeds[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1272 -1283 . DOI: 10.3969/j.issn.1006-267x.2020.03.035

References

[1] 王勇胜,曹玉凤,李秋凤,等.饲粮全棉籽比例对荷斯坦公牛育肥性能、血清生化指标和养分表观消化率的影响[J].动物营养学报,2018,30(5):1965-1972.
[2] GOMES V S,MANO S B,FREITAS M Q,et al.The effect of feed intake containing whole cottonseed on blood parameters of Nellore bulls[J].Arquivo Brasileiro de Medicina Veterinária e Zootecnia,2017,69(3):551-558.

[3] NOFTSGER S M,HOPKINS B A,DIAZ D E,et al.Effect of whole and expanded-expelled cottonseed on milk yield and blood gossypol[J].Journal of Dairy Science,2000,83(11):2539-2547.

[4] DAYANI O,GHORBANI G R,ESMAILIZADEH A K.Supplementation with whole cottonseed changes milk composition and milk fatty acid profile in dairy cows[J].Animal Production Science,2011,51(2):95-101.

[5] BLEVINS S,SIEGEL P B,BLODGETT D J,et al.Effects of silymarin on gossypol toxicosis in divergent lines of chickens[J].Poultry Science,2010,89(9):1878-1886.

[6] KHAN N A,PENG Q,XIN H,et al.Vibrational spectroscopic investigation of heat-induced changes in functional groups related to protein structural conformation in camelina seeds and their relationship to digestion in dairy cows[J].Animal Production Science,2015,55(2):201-206.

[7] HE J E,LECHUGA M E T,LEI Y G,et al.Protein molecular structural,physicochemical and nutritional characteristics of warm-season adapted genotypes of sorghum grain:impact of heat-related processing[J].Journal of Cereal Science,2019,85:182-191.
[8] XIN H S,DING X,ZHANG L Y,et al.Investigation of the spectroscopic information on functional groups related to carbohydrates in different morphological fractions of corn Stover and their relationship to nutrient supply and biodegradation characteristics[J].Journal of Agricultural and Food Chemistry,2017,65(20):4035-4043.

[9] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:National Academy Press,2001.
[10] 国家技术监督局.GB/T 6432-1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,1994.
[11] LICITRA G,HERNANDEZ T M,VAN SOEST P J.Standardization of procedures for nitrogen fractionation of ruminant feeds[J].Animal Feed Science and Technology,1996,57(4):347-358.

[12] ROE M B.Techniques for measuring protein fractions in feedstuffs[C]//Proceedings of Conference for Feed Manufacturers.Department of Animal and Poultry and Avian Sciences.New York:Cornell University,1990:81-88.
[13] RUSSELL J B,O'CONNOR J D,FOX D G,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅰ.Ruminal fermentation[J].Journal of Animal Science,1992,70(11):3551-3561.

[14] NUEZ-ORTÍN W G,YU P Q.Estimation of ruminal and intestinal digestion profiles,hourly effective degradation ratio and potential N to energy synchronization of co-products from bioethanol processing[J].Journal of the Science of Food and Agriculture,2010,90(12):2058-2067.
[15] GARGALLO S,CALSAMIGLIA S,FERRET A.Technical note:a modified three-step in vitro procedure to determine intestinal digestion of proteins[J].Journal of Animal Science,2006,84(8):2163-2167.

[16] ØRSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92(2):499-503.

[17] PUBLISHING S.Base SAS 9.2 procedures guide:statistical procedures[M]//Base SAS 9.2 procedures guide:statistical procedures.[S.l.]:SAS Publishing,2008.
[18] JAEGER H,JANOSITZ A,KNORR D.The Maillard reaction and its control during food processing.The potential of emerging technologies[J].Pathologie Biologie,2010,58(3):207-213.

[19] TAGHINEJAD-ROUDBANEH M,KAZEMI-BONCHENARI M,SALEM A Z M,et al.Influence of roasting,gamma ray irradiation and microwaving on ruminal dry matter and crude protein digestion of cottonseed[J].Italian Journal of Animal Science,2016,15(1):144-150.

[20] AHMAD K N,BOOKER H,YU P Q.Effect of heating method on alteration of protein molecular structure in flaxseed:relationship with changes in protein subfraction profile and digestion in dairy cows[J].Journal of Agricultural and Food Chemistry,2015,63(4):1057-1066.

[21] PRATES L L,LEI Y G,REFAT B,et al.Effects of heat processing methods on protein subfractions and protein degradation kinetics in dairy cattle in relation to protein molecular structure of barley grain using advanced molecular spectroscopy[J].Journal of Cereal Science,2018,80:212-220.
[22] 李昕,徐宏建,王楠,等.不同热处理豆渣中蛋白质分子结构与其营养价值及瘤胃降解特性的相关性研究[J].动物营养学报,2019,31(1):395-408.
[23] 徐宏建,李昕,么恩悦,等.不同热处理DDGS蛋白质营养价值及瘤胃降解特性与其分子结构的相关性研究[J].中国畜牧杂志,2019,55(1):73-80.
[24] HUANG X W,KHAN N A,ZHANG X W,et al.Effects of canola meal pellet conditioning temperature and time on ruminal and intestinal digestion,hourly effective degradation ratio,and potential nitrogen to energy synchronization in dairy cows[J].Journal of Dairy Science,2015,98(12):8836-8845.

[25] GHANBARI F,GHOORCHI T,SHAWRANG P,et al.Comparison of electron beam and gamma ray irradiations effects on ruminal crude protein and amino acid degradation kinetics,and in vitro digestibility of cottonseed meal[J].Radiation Physics and Chemistry,2012,81(6):672-678.

[26] TAGHINEJAD M,NIKKHAH A,SADEGHI A A,et al.Effects of gamma irradiation on chemical composition,antinutritional factors,ruminal degradation and in vitro protein digestibility of full-fat soybean[J].Asian-Australasian Journal of Animal Sciences,2009,22(4):534-541.

[27] CHRENKOVÁ M,?EREŠ?ÁKOVÁ Z,FORMELOVÁ Z,et al.Chemical and nutritional characteristics of different types of DDGS for ruminants[J].Journal of Animal and Feed Sciences,2012,21(3):425-435.

[28] YU P.Application of advanced synchrotron radiation-based Fourier transform infrared (SR-FTIR) microspectroscopy to animal nutrition and feed science:a novel approach[J].British Journal of Nutrition,2004,92(6):869-885.

[29] PENG Q H,KHAN N A,WANG Z S,et al.Moist and dry heating-induced changes in protein molecular structure,protein subfractions,and nutrient profiles in camelina seeds[J].Journal of Dairy Science,2014,97(1):446-457.

[30] 李欣新.双低菜粕和豆粕分子结构与营养特性和奶牛生产性能的关系[D].博士学位论文.哈尔滨:东北农业大学,2016:61-65.
[31] 丁雪,张幸怡,郝小燕,等.玉米秸秆不同部位的蛋白质营养价值与其分子结构相关关系的研究[J].畜牧兽医学报,2016,47(8):1592-1600.
[32] ZHANG X W,YU P Q.Relationship of carbohydrate molecular spectroscopic features in combined feeds to carbohydrate utilization and availability in ruminants[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2012,92:225-233.
[33] 张美美.膨化和发酵大豆分子结构FTIR分析及其对瘤胃降解特性影响[D].硕士学位论文.哈尔滨:东北农业大学,2018:13-15.

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