Effects of Dietary Methionine Level on Growth Performance, Muscle Quality and Muscle Synthesis Pathway Related Gene Expression of Songpu Mirror Carp

CHENG Long; WANG Liansheng; XU Qiyou

doi:10.3969/j.issn.1006-267x.2020.03.037

2020 , Vol. 32 >Issue 3: 1293 - 1304

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.037

Effects of Dietary Methionine Level on Growth Performance, Muscle Quality and Muscle Synthesis Pathway Related Gene Expression of Songpu Mirror Carp

CHENG Long ,
WANG Liansheng ,
XU Qiyou

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1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China;
3. Zhejiang Provincial Key Laboratory of Aquatic Bioresource Conservation and Development Technology, College of Life Sciences, Huzhou Normal University, Huzhou 313000, China

Received date: 2019-09-23

Online published: 2020-03-13

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Abstract

This experiment was conducted to study the effects of dietary methionine (Met) level on growth performance, muscle quality and muscle synthesis pathway related gene expression of Songpu mirror carp. A total of 540 juvenile Songpu mirror carp with the initial body weight of (24.42±0.19) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in 6 groups were fed the isonitrogenous and isoenergetic diets with the Met levels of 0.56% (control), 0.66%, 0.75%, 0.83%, 0.92% and 1.01%, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) the weight gain rate, specific growth rate, protein efficiency ratio and condition factor of 0.83%, 0.92% and 1.01% groups were significantly higher than those of the control group (P<0.05), and the feed conversion ratio was significantly lower than that of the control group (P<0.05). 2) The muscle moisture content of 0.83%, 0.92% and 1.01% groups was significantly lower than that of the control group (P<0.05), the muscle crude protein content of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in muscle of 0.92% and 1.01% groups were significantly lower than those of the control group (P<0.05). 3) The gene expression of insulin-like growth factor (IGF-Ⅰ) in fast-twitch muscle and slow-twitch muscle of 0.83% and 0.92% groups was significantly higher than that of the control group (P<0.05), the gene expression of mammalian target of rapamycin (mTOR) in fast-twitch muscle and slow-twitch muscle of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of serine/threonine protein kinase 11 (STK11) in slow-twitch muscle of 0.66%, 0.75%, 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of phosphoinositide-3-kinase regulatory subunit 3 (PIK3R3) in fast-twitch muscle of 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), the gene expression of myosin heavy chain (MHC) in fast-twitch muscle and slow-twitch muscle of 0.66%, 0.75%, 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05), and the gene expression of nuclear factor E2-related factor 2 (Nrf2) in fast-twitch muscle and slow-twitch muscle of 0.83%, 0.92% and 1.01% groups was significantly higher than that of the control group (P<0.05). In conclusion, the addition of Met in diet can improve the growth and muscle quality of carp, and promote expression of muscle synthesis pathway related gene. When dietary cystine level is 0.64%, based on specific growth rate and protein efficiency ratio, using the broken-line model analysis indicate that the Met requirement of juvenile Songpu mirror carp is 0.90%.

Key words： methionine; Songpu mirror carp; muscle; quality; synthesis pathway; gene expression

Cite this article

CHENG Long , WANG Liansheng , XU Qiyou . Effects of Dietary Methionine Level on Growth Performance, Muscle Quality and Muscle Synthesis Pathway Related Gene Expression of Songpu Mirror Carp[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1293 -1304 . DOI: 10.3969/j.issn.1006-267x.2020.03.037

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