Effects of Methionine on Growth Performance, Serum Biochemical Indices and Liver Enzyme Activity of Epinephelus coioides in Two Sizes

YANG Xuanyi; WANG Xuewu; CHI Shuyan; TAN Beiping; DONG Xiaohui; YANG Qihui; LIU Hongyu; ZHANG Shuang

doi:10.3969/j.issn.1006-267x.2020.03.038

2020 , Vol. 32 >Issue 3: 1305 - 1314

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.038

Effects of Methionine on Growth Performance, Serum Biochemical Indices and Liver Enzyme Activity of Epinephelus coioides in Two Sizes

YANG Xuanyi ,
WANG Xuewu ,
CHI Shuyan ,
TAN Beiping ,
DONG Xiaohui ,
YANG Qihui ,
LIU Hongyu ,
ZHANG Shuang

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1. Laboratory of Aquatic Animal Nutrition and Feed, Guangdong Ocean University, Zhanjiang 524088, China;
2. Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang 524088, China;
3. Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province, Zhanjiang 524088, China;
4. Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang), Zhanjiang 524088, China

Received date: 2019-09-12

Online published: 2020-03-13

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Abstract

This study was conducted to determine the effects of dietary methionine (Met) level on the growth performance, serum biochemical indices and liver enzyme activity for small size[initial weight (9.75±0.05) g] and large size[initial weight (102.60±0.14) g] of Epinephelus coioides. Exploring the Met requirement and the difference in nutrient utilization ability for two sizes of Epinephelus coioides. Eight iso-nitrogenous and iso-lipid diets were prepared with DL-Met supplement at different levels, 0.70% (0.7M), 0.85% (0.85M), 1.00% (1.0M), 1.20% (1.2M), 1.40% (1.4M), 1.60% (1.6M), 1.90% (1.9M), and 2.20% (2.2M), respectively. Epinephelus coioides of the same size were randomly divided into 6 groups with 3 replicates in each group. Each replicate of the small size Epinephelus coioides were stocked with 30 fish and fed diets of 0.7M, 1.0M, 1.2M, 1.6M, 1.9M and 2.2M for 8 weeks, respectively. Large size Epinephelus coioides were stocked with 20 fish and fed diets of 0.7M, 0.85M, 1.0M, 1.2M, 1.4M and 1.6M for 10 weeks, respectively. The results showed as follows:1) the weight gain rate and protein efficiency ratio of the small size Epinephelus coioides were significantly higher than those of the larger size Epinephelus coioides (P<0.05). 2) The contents of serum total protein, total cholesterol and triglyceride in 0.7M group of small size Epinephelus coioides were significantly lower than those of large size Epinephelus coioides (P<0.05). The content of serum glucose in 0.7M group of small size Epinephelus coioides was significantly higher than that of large size Epinephelus coioides (P<0.05). 3) The activities of liver glutamic-oxaloacetic transaminase and phosphoenolpyruvate carboxylase kinase in 1.2M group of small size Epinephelus coioides were significantly lower than those of large size Epinephelus coioides (P<0.05). The activities of liver superoxide dismutase, catalase and glutamic-pyruvic transaminase in 1.2M group of small size Epinephelus coioides were significantly higher than those of large size Epinephelus coioides (P<0.05). Quadratic regression analysis of the specific growth rate corresponding to the level of dietary Met indicate that the optimal level for dietary Met in small size and large size Epinephelus coioides are 3.40% of diet (3.16% of the diet protein) and 1.07% of diet (2.38% of the diet protein). Compared with the large size Epinephelus coioides, the small size Epinephelus coioides has a weaker ability to regulate glucose. With increasing levels of Met, the utilization of sugar and protein for small size Epinephelus coioides are improved.

Key words： Epinephelus coioides; methionine; two sizes; growth performance; nutrient utilization

Cite this article

YANG Xuanyi , WANG Xuewu , CHI Shuyan , TAN Beiping , DONG Xiaohui , YANG Qihui , LIU Hongyu , ZHANG Shuang . Effects of Methionine on Growth Performance, Serum Biochemical Indices and Liver Enzyme Activity of Epinephelus coioides in Two Sizes[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1305 -1314 . DOI: 10.3969/j.issn.1006-267x.2020.03.038

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