Effects of Lentinan on Expression of Inflammation and Protein Degradation-Related Genes in Muscle of Weaned Piglets Challenged by Lipopolysaccharide

WAN Zhicheng; WANG Longmei; ZHANG Lin; QIN Qin; WANG Xiuying; ZHU Huiling; LIU Yulan; KANG Ping

doi:10.3969/j.issn.1006-267x.2020.03.040

2020 , Vol. 32 >Issue 3: 1327 - 1333

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.040

Effects of Lentinan on Expression of Inflammation and Protein Degradation-Related Genes in Muscle of Weaned Piglets Challenged by Lipopolysaccharide

WAN Zhicheng ,
WANG Longmei ,
ZHANG Lin ,
QIN Qin ,
WANG Xiuying ,
ZHU Huiling ,
LIU Yulan ,
KANG Ping

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2019-09-23

Online published: 2020-03-13

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Abstract

This experiment was conducted to investigate the effects of lentinan (LNT) on the expression of inflammation and protein degradation-related genes in muscle of weaned piglets challenged by lipopolysaccharide (LPS). Twenty-four healthy weaned piglets with the body weight of (7.84±0.21) kg were randomly divided into 2 groups According to the similar body weight principle:control group (n=12) and LNT group (n=12). Weaned piglets in the control group were fed a basal diet, and those in LNT group were fed the basal diet+0.02% LNT. After feeding 28 days, six piglets in each group were selected and injected intraperitoneally with LPS at 100 μg/kg BW, and the others in each group were injected intraperitoneally with 0.9% saline at the same dosage. At 4 h after injection, piglets were sacrificed for muscle samples collection. The muscle samples were used to detected the mRNA expression levels of key genes in Toll-like receptor 4 (TLR4), nucleotide binding oligomerization domain (NOD) and serine-threonine kinase (Akt)/forkhead transcription factor (FOXO) signaling pathways. The results showed as follows:1) after LPS injection, the mRNA expression levels of TLR4, bone marrow differentiation factor 88 (MyD88), NOD2, receptor-interacting serine/threonine protein kinase 2 (RIPK2) and tumor necrosis factor-α (TNF-α) were significantly up-regulated in longissimus dorsi and gastrocnemius muscles (P<0.05), and the mRNA expression level of nuclear factor-kappa B (NF-κB) in gastrocnemius muscle was significantly up-regulated (P<0.05). The mRNA expression levels of MyD88, TNF-α in longissimus dorsi muscle and TLR4, RIPK2 and NF-κB in gastrocnemius muscle were significantly up-regulated after adding lentinan (P<0.05). 2) After LPS injection, the mRNA expression levels of FOXO1 and muscle ring finger protein 1 (MuRF1) in longissimus dorsi and gastrocnemius muscles were significantly up-regulated (P<0.05), while the mRNA expression level of Akt1 was significantly down regulated (P<0.05). When LNT was added, the mRNA expression level of muscle atrophy F-box (MAFbx) in gastrocnemius muscle was significantly down regulated (P<0.05). These results suggest that adding 0.02% LNT to the diet of weaned piglets can enhance immunity by further activating TLR4 and NOD signaling pathways in muscle tissue of weaned piglets induced by LPS stimulation in acute phase of stress, and regulate the expression of Akt/FOXO signaling pathway-related genes, therefore slow down the degradation of muscle protein.

Key words： lentinan; lipopolysaccharide; weaned piglet; muscle; TLR4 signaling pathway; NOD signaling pathway; Akt/FOXO signaling pathway

Cite this article

WAN Zhicheng , WANG Longmei , ZHANG Lin , QIN Qin , WANG Xiuying , ZHU Huiling , LIU Yulan , KANG Ping . Effects of Lentinan on Expression of Inflammation and Protein Degradation-Related Genes in Muscle of Weaned Piglets Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1327 -1333 . DOI: 10.3969/j.issn.1006-267x.2020.03.040

References

[1] CHEN F,LIU Y L,ZHU H L,et al.Fish oil attenuates liver injury caused by LPS in weaned pigs associated with inhibition of TLR4 and nucleotide-binding oligomerization domain protein signaling pathways[J].Innate Immunity,2013,19(5):504-515.

[2] WANG Y,HAN X,LI Y D,et al.Effects of tumor-specific antigen induced by lentinan on murine H22 hepatocellular carcinoma immunoprophylaxis[J].European Review for Medical and Pharmacological Sciences,2015,19(23):4516-4524.
[3] INA K,KATAOKA T,ANDO T.The use of lentinan for treating gastric cancer[J].Anti-Cancer Agents in Medicinal Chemistry,2013,13(5):681-688.

[4] 侯爱萍,张树梅.香菇多糖抗菌抗病毒普适性研究[J].药学研究,2015,34(4):199-201.
[5] 艾亮,王晓东.香菇多糖联合云芝糖肽对Lewis肺癌小鼠免疫调节与抗肿瘤作用的研究[J].新中医,2019,51(1):12-17.
[6] 刘国欣,邢建国,李明春,等.香菇多糖对小鼠T淋巴细胞膜上离子通道基因表达的影响[J].中国药房,2014,25(15):1361-1364.
[7] 汪龙梅.香菇多糖对脂多糖刺激的断奶仔猪肠道损伤的调控作用[D].硕士学位论文.武汉:武汉轻工大学,2018.
[8] FANZANI A,CONRAADS V M,PENNA F,et al.Molecular and cellular mechanisms of skeletal muscle atrophy:an update[J].Journal of Cachexia,Sarcopenia and Muscle,2012,3(3):163-179.

[9] ORELLANA R A,SURYAWAN A,WILSON F A,et al.Development aggravates the severity of skeletal muscle catabolism induced by endotoxemia in neonatal pigs[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2012,302(6):R682-R690.
[10] 陈逢.鱼油通过TLR4和NOD信号通路对脂多糖诱导的仔猪肠道、肝脏损伤和肌肉蛋白质降解的调控作用[D].硕士学位论文.武汉:武汉轻工大学,2013.
[11] CHEN S K,LIU Y L,WANG X Y,et al.Asparagine improves intestinal integrity,inhibits TLR4 and NOD signaling,and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge[J].Innate Immunity,2016,22(8):577-587.

[12] FUKATA M,VAMADEVAN A S,ABREU M T.Toll-like receptors (TLRs) and NOD-like receptors (NLRs) in inflammatory disorders[J].Seminars in Immunology,2009,21(4):242-253.

[13] 陈少魁,刘玉兰,李权,等.脂多糖刺激对仔猪下丘脑-垂体-肾上腺轴Toll样受体4信号通路关键基因表达的影响[J].动物营养学报,2014,26(11):3356-3361.
[14] TAKEUCHI O,AKIRA S.Pattern recognition receptors and inflammation[J].Cell,2010,140(6):805-820.

[15] MURATA Y,SHIMAMURA T,TAGAMI T,et al.The skewing to Th1 induced by lentinan is directed through the distinctive cytokine production by macrophages with elevated intracellular glutathione content[J].International Immunopharmacology,2002,2(5):673-689.

[16] AHN H,JRON E,KIM J C,et al.Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production[J].Scientific Reports,2017,7:1314.
[17] LAUFENBERG L J,PRUZNAK A M,NAVARATNARAJAH M,et al.Sepsis-induced changes in amino acid transporters and leucine signaling via mTOR in skeletal muscle[J].Amino Acids,2014,46(12):2787-2798.

[18] DEHOUX M,VAN BENEDEN R,PASKO N,et al.Role of the insulin-like growth factor Ⅰ decline in the induction of atrogin-1/MAFbx during fasting and diabetes[J].Endocrinology,2004,145(11):4806-4812.

[19] KANG P,WANG X Y,WU H T,et al.Glutamate alleviates muscle protein loss by modulating TLR4,NODs,Akt/FOXO and mTOR signaling pathways in LPS-challenged piglets[J].PLoS One,2017,12(8):e0182246.

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