Differences of Intestinal Digestive Enzyme Activities and Ileal Zonula Occludens-1 and Occludin Gene Expression of Different Breeds of Pigeon Squabs

MA Xiaowen; TIAN Yong; LI Guoqin; XU Qianqian; CHEN Xiaoyan; TAO Zhengrong; WEN Jihui; CUI Shihao; LU Lizhi; ZOU Xiaoting

doi:10.3969/j.issn.1006-267x.2020.03.041

2020 , Vol. 32 >Issue 3: 1334 - 1343

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.041

Differences of Intestinal Digestive Enzyme Activities and Ileal Zonula Occludens-1 and Occludin Gene Expression of Different Breeds of Pigeon Squabs

MA Xiaowen ,
TIAN Yong ,
LI Guoqin ,
XU Qianqian ,
CHEN Xiaoyan ,
TAO Zhengrong ,
WEN Jihui ,
CUI Shihao ,
LU Lizhi ,
ZOU Xiaoting

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1. College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Key Laboratory of Animal Nutrition and Feed Sciences in East China of Ministry of Agriculture, Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Hangzhou 310058, China;
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Pingyang County Aofeng Pigeon Industry Co., Ltd., Wenzhou 325400, China;
4. Zhongnan Bai Grassland Group Co., Ltd., Leading Innovation Team Project of South Taihu Elite Program, Huzhou 313300, China

Received date: 2019-09-23

Online published: 2020-03-13

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Abstract

The purpose of this study was to compare the differences of intestinal digestive enzyme activities and ileum zonula occludens-1 (ZO-1) and Occludin gene expression of different breeds of pigeon squabs. Each breeds of white king pigeons, carneau pigeons and Aofeng pigeons at 1 day of age were selected and contained 120 pigeon squabs, three breeds were three groups, each group contained 6 replicates and 20 pigeon squabs per replicate. The experiment lasted for 21 days. The body weight, activities of lipase, sucrase, trypsin, chymotrypsin, maltase in duodenum and jejunum, as well as the gene expressions of ileal tight junction protein ZO-1, Occludin of three breeds of pigeon squabs at 1, 7, 14 and 21 days of age were detected. The results showed as follows:1) there was no significant difference in body weight of pigeon squabs among breeds (P>0.05). 2) The activities of chymotrypsin and trypsin in duodenum of carneau pigeons at 1 day of age were significantly higher than those of white king pigeons (P<0.05), the duodenum lipase activity of white king pigeons and Aofeng pigeons at 7 days of age was significantly higher than that of carneau pigeons (P<0.05), and the duodenum sucrase activity of Aofeng pigeons and duodenum sucrose and maltase activities of white king pigeons at 14 days of age were significantly higher than those of carneau pigeons (P<0.05). 3) The jejunum chymotrypsin activity of carneau pigeons at 1 day of age was significantly higher than that of white king pigeons (P<0.05), the jejunum sucrase activity was significantly higher than that of Aofeng pigeons (P<0.05), and the activities of lipase and maltase in jejunum were significantly higher than those of white king pigeons and Aofeng pigeons (P<0.05). The activities of trypsin, sucrase and maltase in jejunum of white king pigeons at 14 days of age were significantly higher than those of carneau pigeons (P<0.05), and the activities of chymotrypsin, sucrase and lipase in jejunum of white king pigeons at 21 days of age were significantly higher than those of carneau pigeons (P<0.05). 4) There was no significant difference in gene expression levels of ZO-1 and Occludin in ileum of pigeon squabs between breeds (P>0.05). 5) The body weight of different breeds of pigeon squabs was linearly increased with the day of age increased (P<0.05), the activities of sucrase, maltase and lipase in duodenum and jejunum were linearly increased with the day of age increased (P<0.05), and the gene expression levels of ZO-1 and Occludin in ileum were linearly increased with the day of age increased (P<0.05). In conclusion, the body weight of white king pigeons, carneau pigeons and Aofeng pigeons are linearly increased with the day of age increased, but there was no significant difference among different breeds at the same day of age. Different breeds of pigeon squabs have different digestive characteristics at different developmental stages. The gene expression levels of ZO-1 and Occludin in ileum of different breeds of pigeon squabs are linearly increased with the day of age increased, but there was no significant difference among different breeds at the same day of age.

Key words： breeds; days of age; pigeon squabs; body weight; digestive enzyme; tight junction protein

Cite this article

MA Xiaowen , TIAN Yong , LI Guoqin , XU Qianqian , CHEN Xiaoyan , TAO Zhengrong , WEN Jihui , CUI Shihao , LU Lizhi , ZOU Xiaoting . Differences of Intestinal Digestive Enzyme Activities and Ileal Zonula Occludens-1 and Occludin Gene Expression of Different Breeds of Pigeon Squabs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1334 -1343 . DOI: 10.3969/j.issn.1006-267x.2020.03.041

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