The purpose of this study was to explore the optimal combination zymogram of 6 kinds of non-starch polysaccharide enzymes (xylanase, β-glucanase, cellulase, β-mannanase, α-galactosidase and pectase) added to broilers' corn-soybean meal-miscellaneous meal diet by response surface method. The in vitro digestion test simulated gastrointestinal fluid by the third generation monogastric animal simulate digestive system (SDS-Ⅲ). Using a single factor completely random design, every 5 levels of 6 non-starch polysaccharide enzymes were added to the corn-soybean meal-miscellaneous meal basal diet for broilers and each level was repeated for 5 times. The best addition level of single enzyme was determined by the reducing sugar release (RS) and improved dry matter digestibility (IDMD). Based on the above results, the software Designed-Expert 8.06 Box-Behnken response surface method was further used to design the 6 factors and 3 level L₅₄ (3⁶) test, and the 6 kinds of enzymes were compounded and combined. RS and IDMD were used as response values to determine the optimal combination enzyme spectrum of 6 kinds of enzymes. The results showed that the optimized zymogram of 6 kinds of non-starch polysaccharide enzymes in corn-soybean meal-miscellaneous meal diet for broilers aged 1 to 3 weeks was 11.40 U/g xylanase, 3.76 U/g β-glucanase, 8.52 U/g cellulase, 8.19 U/g β-manganase, 6.24 U/g α-galactosidase and 1.60 U/g pectase, and the RS and IDMD were 9.71 mg/g and 2.86% respectively by the reaction catalyzed by this zymogram. Under this condition, the experiment was repeated 3 times, the RS and IDMD were 9.59 mg/g and 2.81% respectively, and the error with the theoretical optimal value were 1.24% and 1.75%, respectively. These showed that the zymogram could reflect the good results of RS and IDMD. The optimized zymogram of 6 kinds of non-starch polysaccharide enzymes in corn-soybean meal-miscellaneous meal diet for broilers aged 4 to 6 weeks was 11.90 U/g xylanase, 5.26 U/g β-glucanase, 8.32 U/g cellulase, 7.96 U/g β-manganase, 6.29 U/g α-galactosidase and 6.17 U/g pectase, and the RS and IDMD were 10.45 mg/g and 2.95% respectively by the reaction catalyzed by this zymogram. Under this condition, the experiment was repeated 3 times, the RS and IDMD were 10.34 mg/g and 2.92% respectively, and the error with the theoretical optimal value were 1.05% and 1.02%, respectively. These showed that the zymogram could reflect the good results of RS and IDMD. To sum up, the optimal zymogram of 6 kinds of non-starch polysaccharide enzymes in diet for broilers aged 1 to 3 weeks was 11.40 U/g xylanase, 3.76 U/g β-glucanase, 8.52 U/g cellulase, 8.19 U/g β-manganase, 6.24 U/g α-galactosidase and 1.60 U/g pectase, and in diet for broilers aged 4 to 6 weeks was 11.90 U/g xylanase, 5.26 U/g β-glucanase, 8.32 U/g cellulase, 7.96 U/g β-manganase, 6.29 U/g α-galactosidase and 6.17 U/g pectase.