Effects of Forage Grass in Different Phenological Periods on Serum Biochemical Indexes, Ruminal Fermentation Parameters and Rumen Microbial Function Flora of Tibetan Sheep

LIU Hongjin; XU Shixiao; HAN Xueping; MA Li; HU Linyong; ZHAO Na; XU Tianwei; KANG Shengping

doi:10.3969/j.issn.1006-267x.2020.03.046

2020 , Vol. 32 >Issue 3: 1396 - 1404

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.046

Effects of Forage Grass in Different Phenological Periods on Serum Biochemical Indexes, Ruminal Fermentation Parameters and Rumen Microbial Function Flora of Tibetan Sheep

LIU Hongjin ,
XU Shixiao ,
HAN Xueping ,
MA Li ,
HU Linyong ,
ZHAO Na ,
XU Tianwei ,
KANG Shengping

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1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China;
2. Key Laboratory of Adaption and Evolution of Plateau Biota, Chinese Academy of Sciences, Xining 810008, China;
3. University of Chinese Academy of Science, Beijing 100049, China

Received date: 2019-09-19

Online published: 2020-03-13

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Abstract

The aim of this experiment was to study the effects of forage grass in different phenological periods on serum biochemical indexes, ruminal fermentation parameters and rumen microbial function flora of Tibetan sheep. The forge grass were collected and the common nutrition contents were determined during the regreen period, grassy period and withering period, respectively. Ten yearlong range females Tibetan sheep at four years of age with body weight of (34.08±2.94) kg were selected and marked using ear tags with numbers. Before free-range with empty stomach in regreen period, grassy period and withering period, the jugular vein blood and rumen fluids were collected to measure the serum biochemical indexes, ruminal fermentation parameters and rumen microbial function flora, respectively. The results showed as follows:1) the crude protein and ether extract contents of forage grass in grassy period were significantly higher than those in regreen and withering periods (P<0.05), whereas the acid detergent fiber content in withering period was remarkably higher than that in the other periods (P<0.05), and natural detergent fiber content was remarkably higher than that in regreen period (P<0.05). 2) The serum contents of total protein (TP), albumin (ALB) and alanine transaminase (ALT) activity in withering period were significantly lower than those in regreen and grassy periods (P<0.05), whereas no significantly different in the contents of triglyceride (TG), low density lipoprotein (LDL) and growth hormone (GH) (P>0.05). 3) The ruminal fluid contents of ammonia nitrogen, acetate, isobutyric acid, valeric acid and propionate percentage reached the highest in grassy period, whereas the acetate percentage in withering period was the highest, and the ratio of acetate to propionate was significantly higher than that in regreen and grassy periods (P<0.05). 4) Metagenomic analysis showed that bacteria which accounted for about 70% of total microorganisms was the highest in the rumen of Tibetan sheep, followed by fungus (20%) and the other unassigned microorganisms (10%). In addition, the main cellulose decomposition bacteria in rumen fluids were Prevotellla ruminicola, Ruminococcus albus and Fibrobacter succinogenes and their relative abundance were significantly higher than those in the other two phenological periods (P<0.05), whereas the starch decomposition bacteria of Selenomonas ruminantium in grassy period was significantly higher than that in other phenological period (P<0.05). In conclusion, the changes of common nutrition of forage grass change the serum biochemical indexes, rumen fermentation parameters and some functional bacteria of Tibetan sheep; the analysis of serum biochemical indexes and rumen fermentation parameters showing that Tibetan sheep have weak resistance to diseases in cold season (regreen and withering periods), therefor, the pastoralists should make reasonable supplementary feeding to Tibetan sheep to improve their growth performance.

Key words： phenological period; Tibetan sheep; forage grass nutrition; serum biochemical indexes; rumen fermentation parameters; rumen microbial function flora

Cite this article

LIU Hongjin , XU Shixiao , HAN Xueping , MA Li , HU Linyong , ZHAO Na , XU Tianwei , KANG Shengping . Effects of Forage Grass in Different Phenological Periods on Serum Biochemical Indexes, Ruminal Fermentation Parameters and Rumen Microbial Function Flora of Tibetan Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1396 -1404 . DOI: 10.3969/j.issn.1006-267x.2020.03.046

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