Effects of Sea Buckthorn Flavone on Gas Production, Rumen Fermentation Parameters and Microflora Population of Sheep in Vitro

BAI Qichang; HAO Xiaoyan; XIANG Binwei; ZHANG Wenjia; ZHANG Hongxiang; ZHANG Jianxin

doi:10.3969/j.issn.1006-267x.2020.03.047

2020 , Vol. 32 >Issue 3: 1405 - 1414

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.047

Effects of Sea Buckthorn Flavone on Gas Production, Rumen Fermentation Parameters and Microflora Population of Sheep in Vitro

BAI Qichang ,
HAO Xiaoyan ,
XIANG Binwei ,
ZHANG Wenjia ,
ZHANG Hongxiang ,
ZHANG Jianxin

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1. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China;
2. Animal Husbandry Bureau of Youyu County, Youyu 037200, China;
3. Shanxi Xianghe Lingshang Farm Animal Husbandry Development Co., Ltd., Youyu 037200, China

Received date: 2019-08-28

Online published: 2020-03-13

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Abstract

The aim of this experiment was to investigate the effects of dietary different supplement levels of sea buckthorn flavone on in gas production, rumen fermentation parameters and microflora population of sheep in vitro through in vitro gas production method. The total mixed ration for fattening sheep with the ratio of concentrate to forage 60:40 was used as fermentation substrate, and the experiment was divided into 6 groups, the supplemental levels of sea buckthorn flavone of 6 groups were 0 (control), 0.1%, 0.2%, 0.3%, 0.4% and 0.5%. After 24 h in vitro fermentation, the gas production, rumen fermentation parameters and relative quantity of rumen microflora were measured. The results showed as follows:1) compared with the control group, dietary supplemented with 0.3%, 0.4% and 0.5% sea buckthorn flavone significantly increased the gas production in each time point (P<0.05), and the gas production reached the maximum value when the supplemental level of seabuckthorn flavone was 0.5%. 2) Compared with the control group, dietary supplemented with 0.3% and 0.4% sea buckthorn flavone significantly increased the dry matter degradation rate, organic matter degradation rate and metabolic energy (P<0.05), and reached the highest when the supplemental level of seabuckthorn flavone was 0.3%; however, there was no significantly difference in organic matter degradation rate and metabolic energy between 0.5% group and control group (P>0.05). 3) Dietary different supplement levels of sea buckthorn flavone had no significant effects on pH and ammoniacal nitrogen concentration in fermentation liquor (P>0.05). The fermentation liquor microbial protein concentration of 0.4% group and 0.5% group was significantly higher than that of other groups (P<0.05); the fermentation liquor methane production of 0.3% group was the lowest, and significantly lower than that of control group, 0.1% group, 0.2% group and 0.5% group (P<0.05). 4) The fermentation liquor acetate concentration was significantly higher than that of control group and 0.1% group (P<0.05), the fermentation liquor propionate concentration was significantly higher than that of other groups (P<0.05); the fermentation liquor total volatile fatty acid of 0.3% group was the highest, and significantly higher than that of control group and 0.1% group (P<0.05). 5) The quantities of Ruminococcus albus, Ruminococcus flavefaciens, protozoa, methanogens and total bacterial in fermentation liquor of 0.4% group and 0.5% group were significantly lower than those of other groups (P<0.05). In summary, sea buckthorn flavone can improve the in vitro rumen fermentation, and inhibit methane production. The optimal supplemental level of sea buckthorn flavone is 0.3% under the condition of this experiment.

Key words： sea buckthorn flavone; in vitro fermentation; methane; microflora

Cite this article

BAI Qichang , HAO Xiaoyan , XIANG Binwei , ZHANG Wenjia , ZHANG Hongxiang , ZHANG Jianxin . Effects of Sea Buckthorn Flavone on Gas Production, Rumen Fermentation Parameters and Microflora Population of Sheep in Vitro[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1405 -1414 . DOI: 10.3969/j.issn.1006-267x.2020.03.047

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