Evaluation of Forage Nutritional Value of Alpine Meadow Grassland in Different Phenological Periods in Zeku County of Qinghai Province by in Vitro Method

  • ZHANG Qunying ,
  • LI Jie ,
  • HAO Lizhuang ,
  • LIU Shujie ,
  • CHAI Shatuo ,
  • NIU Jianzhang ,
  • ZHANG Xiaowei ,
  • WANG Xun ,
  • SUN Lu ,
  • DUOJI Ouzhu
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  • 1. Qinghai University, Xining 810016, China;
    2. Academy of Science and Veterinary Medicine of Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Qinghai Plateau Yak Research Center, Xining 810016, China;
    3. Yadong County Pastoral Service Centers of Tibet Autonomous Region Shigatse, Shigatse 857600, China

Received date: 2019-09-01

  Online published: 2020-03-13

Abstract

The aim of this experiment was to explore the dynamic changes of forage nutritional value of alpine meadow grassland in different phenological periods[January to April (hay period) and May to June (returning green period)] in Zeku county of Qinghai province in order to provide some basic data for accurate supplementary feeding of local grazing livestock. Local typical grazing pastures were chosen to collect edible forage in the middle of each month by tracking grazing yak from January of hay period to June of turning green period, 2018. The conventional nutrient contents of pasture were determined by common nutrient composition analysis method, and the in vitro fermentation method was used to measure the gas production (GP) and in vitro dry matter digestibility (IVDMD) at different fermentation time points, and the pH, ammonia nitrogen (NH3-N) concentration and volatile fatty acid (VFA) contents in fermentation broth at fermentation 48 h. The results showed that the conventional nutrient contents and IVDMD of forage in May and June were significantly higher than those in other months (P<0.01), and the lowest values of them in January. With the nutritional quality of forage changed, 72 h GP and pH, NH3-N concentration and VFA contents in fermentation broth also changed, the 72 h GP and NH3-N concentration in May and June were significantly higher than those in other months (P<0.05), and those in January to March were significantly lower than those in other months (P<0.05 or P<0.01). On the total VFA content in fermentation broth, there was no significant difference between May and June (P>0.05), but they were significantly higher than other months (P<0.01), and the January was significantly lower than other months (P<0.01). In summary, the forage of alpine meadow grassland in Zeku county of Qinghai province has good nutritional quality in turning green period, but it in hay period has lower nutritional quality and can not meet the nutritional needs of grazing yaks. It is recommended for accurate feeding herders, rational use of grassland resources can promote the development of local animal husbandry.

Cite this article

ZHANG Qunying , LI Jie , HAO Lizhuang , LIU Shujie , CHAI Shatuo , NIU Jianzhang , ZHANG Xiaowei , WANG Xun , SUN Lu , DUOJI Ouzhu . Evaluation of Forage Nutritional Value of Alpine Meadow Grassland in Different Phenological Periods in Zeku County of Qinghai Province by in Vitro Method[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1415 -1423 . DOI: 10.3969/j.issn.1006-267x.2020.03.048

References

[1] 权凯,张兆旺.母牦牛的繁殖特性[J].畜牧与饲料科学,2005,26(5):30-32.
[2] 任继周.草业科学研究方法[M].北京:中国农业出版社,1998.
[3] 赵新全,张耀生,周兴民.高寒草甸畜牧业可持续发展:理论与实践[J].资源科学,2000(4):50-61.
[4] 张建勋.不同季节牦牛补饲效果及其机理研究[D].硕士学位论文.雅安:四川农业大学,2013.
[5] 晁文菊.补饲对围产期牦牛生产性能及其犊牦牛生长发育的影响[D].硕士学位论文.西宁:青海大学,2009.
[6] 张胜权.三江源地区草甸草场13种典型牧草营养价值评定研究[D].硕士学位论文.西宁:青海大学,2013.
[7] 满周.浅谈泽库县保护性耕作技术探讨[J].青海农技推广,2018(2):68-69,71.
[8] 杨永胜,张莉,未亚西,等.退化程度对三江源泽库高寒草甸土壤理化性质及持水能力的影响[J].中国草地学报,2017,39(5):54-61.
[9] 马青山,马正炳,张芳,等.青海省泽库县草地生态环境现状及保护建设措施[J].草原与草坪,2009(3):95-97.
[10] 惠文森,陈怀顺,焕角.青海省泽库县草地资源特征及其退化与恢复[J].西北民族大学学报(自然科学版),2007,28(4):43-47.
[11] 王江山,李海红,许正旭.三江源生态环境监测研究[J].气象,2003,29(11):49-51,54.
[12] 全国饲料工业标准化技术委员会.GB/T 6432-1994饲料中粗蛋白测定方法[S].北京:中国标准出版社,1994.
[13] 全国饲料工业标准化技术委员会.GB/T 6438-1992饲料中粗灰分的测定方法[S].北京:中国标准出版社,1992.
[14] VAN SOEST P J.Development of a comprehensive system of feed analyses and its application to forages[J].Journal of Animal Science,1967,26(1):119-128.  
[15] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28(1):7-55.
[16] 冯宗慈,高民.通过比色测定瘤胃液氨氮含量方法的改进[J].畜牧与饲料科学,2010,31(6/7):37.
[17] 曹庆云,周武艺,朱贵钊,等.气相色谱测定羊瘤胃液中挥发性脂肪酸方法研究[J].中国饲料,2006(24):26-28.
[18] 王加启.反刍动物营养学研究方法[M].北京:现代教育出版社,2011:10.
[19] BODAS R,LÓPEZ S,FERNÁNDEZ M,et al.In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants[J].Animal Feed Science and Technology,2008,145(1/2/3/4):245-258.
[20] HALL M B,PELL A N,CHASE L E.Characteristics of neutral detergent-soluble fiber fermentation by mixed ruminal microbes[J].Animal Feed Science and Technology,1998,70(1/2):23-39.
[21] 郑灿财,郭春华,彭忠利,等.牦牛冷季补饲研究进展[J].畜牧与兽医,2014,46(7):113-116.
[22] 康健,匡彦蓓,盛捷.10种作物秸秆的营养品质分析[J].草业科学,2014,31(10):1951-1956.
[23] 段新慧,钟声,李乔仙,等.鸭茅种质资源营养价值评价[J].养殖与饲料,2013(6):38-42.
[24] 杜雪燕,柴沙驼,王迅,等.河南县高山嵩草草地牧草营养价值与载畜量研究[J].河南农业科学,2015,44(11):141-146.
[25] 范小红,杨得玉,郝力壮,等.青海省海晏县牧场牧草营养品质全年动态[J].草业科学,2017,34(11):2359-2365.
[26] 扎西卓玛,王宏博,李桂香,等.高寒草甸草场牧草营养成分测定与分析[J].湖北农业科学,2018,57(1):109-111.
[27] 张盼盼.东苏旗不同季节牧草对放牧羊瘤胃内环境及血液生理生化指标的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2017.
[28] 张均.西藏那曲地区不同月份草地牧草营养价值评定及绒山羊营养补饲研究[D].硕士学位论文.雅安:四川农业大学,2005.
[29] 王克平,娄玉杰,成文革,等.吉生羊草营养物质动态变化规律的研究[J].草业科学,2005,22(8):24-27.
[30] 余苗.不同生长期两种牧草瘤胃降解率及体外发酵产气特性的研究[D].硕士学位论文.长沙:湖南农业大学,2013.
[31] 张中岳.西藏地区牧草营养价值及其瘤胃降解特性的研究[D].硕士学位论文.杨凌:西北农林科技大学,2009.
[32] 薛艳锋,郝力壮,刘书杰.玉树州藏嵩草草地牧草营养价值评定与营养载畜量[J].草业科学,2015,32(10):1660-1667.
[33] 雷冬至,金曙光,乌仁塔娜.用体外产气法评价不同粗饲料与相同精料间的组合效应[J].饲料工业,2009,30(3):30-33.
[34] 郝力壮,王万邦,王迅,等.三江源区嵩草草地枯草期牧草营养价值评定及载畜量研究[J].草地学报,2013,21(1):56-64.
[35] 田丰.加拿大双低油菜籽粕对奶牛瘤胃发酵及产奶性能影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2009.
[36] 张腾,庄苏,董文超,等.不同精粗比日粮对奶山羊瘤胃液pH值、VFA及血液VFA含量的影响[J].畜牧与兽医,2013,45(4):5-10.
[37] GARCÍA-GONZÁLEZ R,GONZÁLEZ J S,LÓPEZ S.Decrease of ruminal methane production in Rusitec fermenters through the addition of plant material from rhubarb (Rheum spp.) and alder buckthorn (Frangula alnus)[J].Journal of Dairy Science,2010,93(8):3755-3763.  
[38] SRINIVAS B,GUPTA B N.Rumen fermentation,bacterial and total volatile fatty acid (TVFA) production rates in cattle fed on urea-molasses-mineral block licks supplement[J].Animal Feed Science and Technology,1997,65(1/2/3/4):275-286.
[39] 王加启.反刍家畜瘤胃内碳水化合物和氮代谢研究的新进展(上)[J].国外畜牧科技,1992,19(4):24-26.
[40] SHI L G,XUN W J,YUE W B,et al.Effect of elemental nano-selenium on feed digestibility,rumen fermentation,and purine derivatives in sheep[J].Animal Feed Science and Technology,2011,163(2/3/4):136-142.
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