Effects of Different Additives on Fermentation Quality and Aerobic Stability of Soybean Milk Type and Soybean Dregs Type Fermented Feeds

  • LI Jinku ,
  • WANG Xueyang ,
  • ZHAO Junxiang ,
  • LIU Chen ,
  • SUN Fengzhao ,
  • QIAO Xinyue ,
  • LI Jingchun ,
  • WEI Guosheng ,
  • LI Yanbing
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  • Key Laboratory of Efficient Utilization and Nutrition Control of Feed Resources in Cold Area of Heilongjiang Province, College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2019-09-05

  Online published: 2020-03-13

Abstract

This experiment was conducted to investigate the effects of different additives on fermentation quality and aerobic stability of soybean milk type and soybean dregs type fermented feeds. The additives contained Lactobacillus buchneri (LB), Lactobacillus plantarum (LP), compound chemical additives 1 and 2. Each group weighed 900 g mixed fermented feed which prepared by corn germ meal, soybean milk or soybean dregs, soybean molasses, sprayed corn husk and rice husk in proportion of 12:45:1:25:17. There were 6 groups in the experiment, which were control group (without any additive), compound chemical additive 1 group (added 4 L/t compound chemical additive 1), compound chemical additive 2 group (added 2 L/t compound chemical additive 2), LB+LP (water) group (added 2.0 mL/kg LP and LB lyophilized powder mixed with water, LB addition was 106 CFU/g, LP addition was 106 CFU/g), LB+LP(MRS) group[added 2.0 mL/kg LP and LB lyophilized powder mixed with lactic acid bacteria culture medium (MRS), LB addition was 106 CFU/g, LP addition was 106 CFU/g] and LB+LP group (added 2.0 mL/kg LP and LB bacteria solution revived by MRS, LB addition was 106 CFU/g, LP addition was 106 CFU/g). Each group contained 3 replicates, and the fermentation cycle of 2 kinds of fermentation feed was 10 days and stored at room temperature. The results showed as follows:1) for soybean dregs type fermented feed, compared with the control group, the dry matter content and Lactobacillus number of compound chemical additive 1 and 2 groups were significantly increased (P<0.05), the contents of lactic acid, propionic acid and isobutyric acid of compound chemical additive 2 and LB+LP groups were significantly decreased (P<0.05), and the Lactobacillus number of experimental groups was significantly increased (P<0.05); the pH at the unsealed day (1 d) of compound chemical additive 1 group was significantly higher than that of other groups (P<0.05), the final (7 d) pH of LB+LP(MRS) group was significantly higher than that of other groups (P<0.05), and the final (7 d) pH of compound chemical additive 2 group was significantly lower than that of other groups (P<0.05). 2) For soybean milk type fermented feed, the pH of compound chemical additive 1 and 2 groups was significantly higher than that of other groups (P<0.05), the dry matter content of compound chemical additive 1 group was significantly higher than that of other groups (P<0.05), the propionic acid content of LB+LP(MRS) group was significantly higher than that of other groups except LB+LP (water) group (P<0.05), and the ethanol content of LB+LP (water) group was significantly higher than that of other groups (P<0.05); the pH at the unsealed day (1 d) of compound chemical additive 2 group was significantly higher than that of other groups (P<0.05), the final (7 d) pH of LB+LP(MRS) group was significantly higher than that of other groups (P<0.05), and the final (7 d) pH of LB+LP (water), LB+LP (MRS) and LB+LP group was significantly higher than that of the control group (P<0.05). In conclusion, all the additives can improve the fermentation quality and aerobic stability of soybean milk type and soybean dregs type fermented feeds to a certain degree, but the compound chemical additive is better than other additives in improving the fermentation quality and aerobic stability of soybean dregs type fermented feed.

Cite this article

LI Jinku , WANG Xueyang , ZHAO Junxiang , LIU Chen , SUN Fengzhao , QIAO Xinyue , LI Jingchun , WEI Guosheng , LI Yanbing . Effects of Different Additives on Fermentation Quality and Aerobic Stability of Soybean Milk Type and Soybean Dregs Type Fermented Feeds[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1424 -1433 . DOI: 10.3969/j.issn.1006-267x.2020.03.049

References

[1] 周曙东,钱悦.美国、巴西大豆生产成本收益比较及对中国的启示[J].世界农业,2018(2):113-118.
[2] 王辽卫.2017年我国饲料原料市场回顾与展望[J].猪业科学,2018,35(2):49-51.
[3] PlUMED-FERRER C,VON WRIGHT A.Fermented pig liquid feed:nutritional,safety and regulatory aspects[J].Journal of Applied Microbiology,2009,106(2):351-368.  
[4] WANG J,HAN Y,ZHAO J Z,et al.Consuming fermented distillers' dried grains with solubles (DDGS) feed reveals a shift in the faecal microbiota of growing and fattening pigs using 454 pyrosequencing[J].Journal of Integrative Agriculture,2017,16(4):900-910.  
[5] KIARIE E,BHANDARI S,SCOTT M,et al.Growth performance and gastrointestinal microbial ecology responses of piglets receiving Saccharomyces cerevisiae fermentation products after an oral challenge with Escherichia coli (K88)[J].Journal of Animal Science,2011,89(4):1062-1078.  
[6] PATRIGNANI F,MODESTO M,MICHELINI S,et al.Technological potential of Bifidobacterium aesculapii strains for fermented soymilk production[J].LWT,2018,89:689-696.
[7] JIANG K,TANG B,WANG Q,et al.The bio-processing of soybean dregs by solid state fermentation using a poly γ-glutamic acid producing strain and its effect as feed additive[J].Bioresource Technology,2019,291:121841.
[8] CHEN Y,YE R,YIN L,et al.Novel blasting extrusion processing improved the physicochemical properties of soluble dietary fiber from soybean residue and in vivo evaluation[J].Journal of Food Engineering,2014,120:1-8.
[9] JIANG Y,ZHAO P F,LIN S M,et al.Partial substitution of soybean meal with fermented soybean residue in diets for juvenile largemouth bass,Micropterus salmoides[J].Aquaculture Nutrition,2018,24(4):1213-1222
[10] YADAV D S,BAGHEL R P S,NAYAK S,et al.Effect of fortified soymilk as partial milk replacer on performance and feeding economics of murrah buffalo calves[J].Journal of Animal Research,2018,8(1):21-25
[11] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:48-80.
[12] PARVIN S,WANG C,LI Y,et al.Effects of inoculation with lactic acid bacteria on the bacterial communities of Italian ryegrass,whole crop maize,guinea grass and rhodes grass silages[J].Animal Feed Science and Technology,2010,160(3/4):160-166.
[13] HAN K J,COLLINS M,VANZANT E S,et al.Bale density and moisture effects on alfalfa round bale silage[J].Crop Science,2004,44(3):914-919.  
[14] 唐庆凤,杨承剑,彭开屏,等.添加植物乳杆菌对桑枝叶与玉米秸秆混合青贮发酵品质的影响[J].饲料工业,2018,39(19):38-43.
[15] 马迪,梁慧慧,邵文强,等.不同乳酸菌添加剂对青贮黑麦草和青贮玉米发酵产物和有氧稳定性的影响[J].草地学报,2014,22(6):1365-1370.
[16] DENEK N,CAN A,AVCI M,et al.The effect of molasses-based pre-fermentedjuice on the fermentation quality of first-cut Lucerne silage[J].Grass and Forage Science,2011,66(2):243-250.  
[17] CHEN L,GUO G,YUAN X J,et al.Effects of applying molasses,lactic acid bacteria and propionic acid on fermentation quality,aerobic stability and in vitro gas production of total mixed ration silage prepared with oat-common vetch intercrop on the Tibetan Plateau[J].Journal of the Science of Food and Agriculture,2016,96(5):1678-1685.  
[18] FERRERO F,PIANO S,TABACCO E,et al.Effects of conservation period and Lactobacillus hilgardii inoculum on the fermentation profile and aerobic stability of whole corn and sorghum silages[J].Journal of the Science of Food and Agriculture,2019,99(5):2530-2540.  
[19] WANG M S,YANG C H,JIA L J,et al.Effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation characteristics and aerobic stability of whipgrass silage in laboratory silos[J].Grassland Science,2014,60(4):233-239.  
[20] DE FARIA PEDROSO A,NUSSIO L G,LOURES D R S,et al.Fermentation,losses,and aerobic stability of sugarcane silages treated with chemical or bacterial additives[J].Scientia Agricola,2008,65(6):589-594.  
[21] RANJIT N K,KUNG L,Jr.The effect of Lactobacillus buchneri,Lactobacillus plantarum,or a chemical preservative on the fermentation and aerobic stability of corn silage[J].Journal of Dairy Science,2000,83(3):526-535.  
[22] 洪梅,刁其玉,姜成钢,等.布氏乳杆菌对青贮发酵及其效果的研究进展[J].草业学报,2011,20(5):266-271.
[23] KLEINSCHMIT D H,SCHMIDT R J,KUNG L,Jr.The Effects of various antifungal additives on the fermentation and aerobic stability of corn silage[J].Journal of Dairy Science,2005,88(6):2130-2139.  
[24] OLIVEIRA A S,WEINBER Z G,OGUNADE I M,et al.Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation,aerobic stability,and the performance of dairy cows[J].Journal of Dairy Science,2017,100(6):4587-4603.  
[25] DA SILVA F B,ROMÃO B B,CARDOSO V L,et al.Production of ethanol from enzymatically hydrolyzed soybean molasses[J].Biochemical Engineering Journal,2012,69:61-68.
[26] 江杨娟,徐丽,陈美思,等.乳酸菌发酵大豆糖蜜生产乳酸及糖代谢变化[J].食品科学,2018,39(6):130-134.
[27] 孟陆丽,刘胜,许金蓉,等.乳酸杆菌发酵大豆糖蜜生产乳酸的研究[J].农业机械,2011(20):148-150.
[28] 林丽芳.添加山梨酸、乙醇对紫花苜蓿和白三叶发酵品质的影响[D].硕士学位论文.南京:南京农业大学,2010:1-47.
[29] TABASZEWSKA M,GABOR A,JAWORSKA G,et al.Effect of fermentation and storage on the nutritional value and contents of biologically-active compounds in lacto-fermented white asparagus (Asparagus officinalis L.)[J].LWT,2018,92:67-72.
[30] KANDASAMY S,KAVITAKE D,SHETTY P H.Lactic acid bacteria and yeasts as starter cultures for fermented foods and their role in commercialization of fermented foods[M]//PANDA S K,SHETTY P H.Innovations in technologies for fermented food and beverage industries.Cham:Springer,2018:25-52.
[31] KUNG L,Jr.,SMITH M L,DA SILVA E B,et al.An evaluation of the effectiveness of a chemical additive based on sodium benzoate,potassium sorbate,and sodium nitrite on the fermentation and aerobic stability of corn silage[J].Journal of Dairy Science,2018,101(7):5949-5960.  
[32] 万江春,谢开云,王玉祥,等.同/异质型乳酸菌添加对苏丹草青贮酵母菌群落结构及发酵品质的影响[J].草业科学,2019,36(2):565-572.
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