Effects of Combined Feed of Spirulina, Schizochytrium and Daphnia magna Powder on Growth, Body Color and Physiological and Biochemical Indexes of Garassius auratus red var.

LIU Mengyu; WU Xuan; ZENG Xiangxi; WANG Yang; YANG Guang; WANG Shuangshuang; ZHU Guoxia; ZHAI Shengli; BAI Dongqing

doi:10.3969/j.issn.1006-267x.2020.03.052

2020 , Vol. 32 >Issue 3: 1454 - 1464

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.03.052

Effects of Combined Feed of Spirulina, Schizochytrium and Daphnia magna Powder on Growth, Body Color and Physiological and Biochemical Indexes of Garassius auratus red var.

LIU Mengyu ,
WU Xuan ,
ZENG Xiangxi ,
WANG Yang ,
YANG Guang ,
WANG Shuangshuang ,
ZHU Guoxia ,
ZHAI Shengli ,
BAI Dongqing

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1. Tianjin Key Lab of Aqua-Ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China;
2. Development Services Center of Animal Husbandry and Fisheries, Jinghai District of Tianjin, Tianjin 301600, China;
3. Tianjin Fisheries Research Institute, Tianjin 300202, China;
4. Tianjin Enterprise Key Lab of the Functional Feed of Aquatic Animals, Tianjin Chenhui Modern Technology Group Co., Ltd., Tianjin 301800, China

Received date: 2019-09-06

Online published: 2020-03-13

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Abstract

This experiment was conducted to study the effects of different supplemental levels of combined feed of Spirulina, Schizochytrium and Daphnia magna powder on the growth, body color and physiological and biochemical indexes of Carassius auratus red var. Three hundred healthy Carassius auratus red var. with the initial body weight of (21.77±1.38) g and the initial body length of (8.79±0.55) cm were selected and divided into 5 groups with 3 replicates per group and 20 fish per replicate, and fish in the 5 groups were fed diets with different levels of combined feed of Spirulina, Schizochytrium, Daphnia magna powder for 60 days, specific as follows:C group (control group), the diet did not contain Spirulina, Schizochytrium and Daphnia magna powder; C1 group, the diet contained 4.0% Spirulina, 1.0% Schizochytrium and 4.0% Daphnia magna powder; C2 group, the diet contained 6.0% Spirulina, 1.5% Schizochytrium and 6.0% Daphnia magna powder; C3 group, the diet contained 8.0% Spirulina, 2.0% Schizochytrium and 8.0% Daphnia magna powder; C4 group, the diet contained 10.0% Spirulina, 2.5% Schizochytrium and 10.0% Daphnia magna powder. Samples were taken on the 30th and 60th day, respectively, to determine the growth indexes, tissue carotenoid content, body color, body physiological and biochemical indexes. The results showed that the protein efficiency ratio (PER), special growth rate (SGR) and weight gain rate (WGR) in C1 and C2 groups were significantly higher than those in C group (P<0.05), and the feed conversion ratio (FCR) was significantly lower than that in C group (P<0.05) at feeding 30 days. At feeding 60 days, the PER, SGR, WGR and condition factor (CF) in C2 and C3 groups were significantly higher than those in C group (P<0.05), and the FCR in C3 group was significantly lower than that in C group (P<0.05). Compared with C group, the carotenoid content in skin and scales in C2 group was significantly increased at feeding 30 days (P<0.05); while at feeding 60 days, the C2 group showed significantly higher carotenoid content in skin and fins (P<0.05). Both at feeding 30 and 60 days, the values of body lightness (L^*) and redness (a^*) reached the highest values in C3 group, which were significantly higher than those in C group (P<0.05); the highest superoxide dismutase (SOD), catalase (CAT)activities and nitric oxide (NO) content and the lowest malondialdehyde (MDA)content in serum and tissues were found in C3 group, as well as the lowest values of serum triglyceride (TG) and total cholesterol (T-CHO) contents, and above indexes in C3 group had significant difference compared with C group (P<0.05). Considering the growth, body color and physiological and biochemical indexes, the suitable additive amount of Spirulina, Schizochytrium and Daphnia magna powder in the diet of Garassius auratus red var. is 8.0%, 2.0% and 8.0%, respectively.

Key words： Garassius auratus red var.; Spirulina; Schizochytrium; Daphnia magna powder; growth; body color; physiological and biochemical indexes

Cite this article

LIU Mengyu , WU Xuan , ZENG Xiangxi , WANG Yang , YANG Guang , WANG Shuangshuang , ZHU Guoxia , ZHAI Shengli , BAI Dongqing . Effects of Combined Feed of Spirulina, Schizochytrium and Daphnia magna Powder on Growth, Body Color and Physiological and Biochemical Indexes of Garassius auratus red var.[J]. Chinese Journal of Animal Nutrition, 2020 , 32(3) : 1454 -1464 . DOI: 10.3969/j.issn.1006-267x.2020.03.052

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