This experiment was conducted to study the effects of dietary zinc level on regenerative down quality, serum indices and expression of genes related to follicle development of goose. A total of 128 healthy Hortobagy geese under the same feeding condition were selected and randomly divided into 4 groups after epilation, with 4 replicates in each group and 8 geese in each replicate, half male and half female. The geese in the four groups were fed diets in which ZnSO₄·H₂O was added in a basal diet with the additive amount of 0, 60, 120 and 180 mg/kg, which equaled zinc additive amount was 0, 21.79, 43.57 and 65.36 mg/kg, respectively. The pre-test lasted for 7 days and the formal test period lasted for 42 days. The results showed as follows:1) dietary added different levels of zinc could increase the kilo-down weight, the down length of chest, abdomen and back of regenerative down, but the differences were not significant (P>0.05). The pinnule diameter of regenerative down was decreased with the dietary zinc additive amount increasing, and when zinc additive amounts reached to 43.57 and 65.36 mg/kg, the pinnule diameter was significantly decreased compared with when zinc additive amounts were 0 and 21.79 mg/kg (P<0.05). 2) The activity of serum alkaline phosphatase (AKP) reached the highest level when zinc additive amount was 65.36 mg/kg, which was significantly higher than that when zinc additive amounts were 0 and 21.79 mg/kg (P<0.05). When zinc additive amount was 43.57 mg/kg, the content of serum triglyceride (TG) reached the highest level, which was significantly higher than that when zinc additive amounts were 0, 21.79 and 65.36 mg/kg (P<0.05). Dietary added different levels of zinc had no significant effects on the contents of total protein (TP), albumin (ALB) and globulin (GLOB) in serum (P>0.05). 3) Dietary added different levels of zinc had significant effects on the concentrations of serum growth hormone (GH) and prolactin (PRL) (P<0.05), but had no significant effect on the concentration of serum insulin-like growth factor-1 (IGF-1) (P>0.05). Serum GH and IGF-1 concentrations were the highest when zinc additive amount reached 21.79 mg/kg, and serum PRL concentration was the highest when zinc additive amount reached 43.57 mg/kg. 4) With the dietary zinc additive amount increasing, the serum zinc content increased firstly and then decreased, and the highest value was found when the zinc additive amount reached 43.57 mg/kg. Serum calcium and iron content showed an upward and downward trend respectively with the dietary zinc additive amount increasing. 5) The mRNA relative expression level of epidermal growth factor (EGF) gene in follicle increased firstly and then decreased with the dietary zinc additive amount increasing, when zinc additive amount reached 43.57 mg/kg, it showed the highest value and significantly higher than that when zinc additive amount was 65.36 mg/kg (P<0.05). The mRNA relative expression level of IGF-1 gene also increased firstly and then decreased with the dietary zinc additive amount increasing, but there was no significant difference among the different additive amounts (P>0.05). In conclusion, the additive amount of 21.79 to 43.57 mg/kg zinc in the basal diet with the zinc level is 31.68 mg/kg to make the dietary zinc level reaches 53.47 to 75.25 mg/kg, can increase the activity of AKP and the concentrations of GH, PRL and IGF-1 in serum, affect serum calcium and iron content, enhance the kilo-down weight and down length of regenerative down, decrease the pinnule diameter of geese. Furthermore, it can regulate the expression of EGF and IGF-1 genes related to follicle development, which can effectively promote down regeneration.