Effects of in Ovo Feeding of L-Arginine on Hatchability, Intestinal Development and Serum Biochemical Indexes of Layer Chicks from 1 to 14 Days of Age

  • DAI Dong ,
  • WANG Jing ,
  • ZHANG Haijun ,
  • WU Shugeng ,
  • QI Guanghai
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  • Key Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2019-10-09

  Online published: 2020-04-16

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Abstract

The objective of this study was to determine the effects of in ovo feeding of L-arginine (L-Arg) on hatchability, intestinal development and serum biochemical indexes of layer chicks from 1 to 14 days of age. A total of 1 080 fertile eggs of 47-week-old Jinghong No.1 layers were randomly assigned to 3 groups including non-injected control (NC) group, saline-injected control (SC) group and L-Arg-injected (Arg) group, with 8 replicates per group and 45 fertile eggs per replicate. At 17.5 days of incubation, each egg in Arg group was injected with 0.1 mL of 10% L-Arg solution (0.85% physiological saline as solvent, 10 mg L-Arg per egg), and eggs in SC group were injected with the same dosage of 0.85% physiological saline. A total of 120 healthy female layer chicks with similar body weight per group were randomly allotted into 8 replicates after hatching. The feeding period was lasted for 14 days. The results showed as follows:1) compared with NC and SC groups, there were no significant effects on hatching rate and hatching weight of layer chicks at 1 day of age by in ovo feeding of L-Arg (P>0.05), but yolk sac weight was significantly decreased (P<0.05). 2) Compared with NC and SC groups, jejunum length at 1 day of age and duodenum length at 3 days of age and jejunum and ileum length of layer chicks at 14 days of age were significantly increased by in ovo feeding of L-Arg (P<0.05), and jejunal contents lipase activity of layer chicks at 3 days of age had a tendency of increase (P=0.084). 3) Compared with NC group, proliferation indexes of jejunal epithelial cells of layer chicks at 14 days of age were significantly increased by in ovo feeding of L-Arg (P<0.05). 4) Compared with NC group, serum triglyceride (TG) content of layer chicks at 3 and 14 days of age was significantly decreased by in ovo feeding of L-Arg (P<0.05), and serum total cholesterol (TC) and low density lipoprotein (LDL) contents at 3 and 14 days of age were significantly increased (P<0.05). Compared with SC group, serum high density lipoprotein (HDL) and glucose (Glu) contents of layer chicks at 14 days of age were significantly increased by in ovo feeding of L-Arg (P<0.05). It is concluded that there are no adverse effects on hatching rate and hatching weight of layer chicks by in ovo feeding of 10 mg L-Arg, in ovo feeding of 10 mg L-Arg promotes lipid metabolism and glucose metabolism to providing more energy for early intestinal development, increase proliferation indexes of jejunal epithelial cells, increase intestinal length and promote early intestinal development of layer chicks.

Cite this article

DAI Dong , WANG Jing , ZHANG Haijun , WU Shugeng , QI Guanghai . Effects of in Ovo Feeding of L-Arginine on Hatchability, Intestinal Development and Serum Biochemical Indexes of Layer Chicks from 1 to 14 Days of Age[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1641 -1649 . DOI: 10.3969/j.issn.1006-267x.2020.04.022

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