Effects of Neutral Detergent Fiber Level of Starter on Tissue Morphology and Digestive Enzyme Activities in Digestive Tract of Lambs

WANG Xiaojuan; LI Fadi; WANG Shiqin; NIAN Fang; LI Chong; WANG Weimin; LIU Ting

doi:10.3969/j.issn.1006-267x.2020.04.029

2020 , Vol. 32 >Issue 4: 1704 - 1712

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.029

Effects of Neutral Detergent Fiber Level of Starter on Tissue Morphology and Digestive Enzyme Activities in Digestive Tract of Lambs

WANG Xiaojuan ,
LI Fadi ,
WANG Shiqin ,
NIAN Fang ,
LI Chong ,
WANG Weimin ,
LIU Ting

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1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
3. College of Science, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2019-10-17

Online published: 2020-04-16

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Abstract

This experiment was conducted to study the effects of neutral detergent fiber (NDF) level of starter on tissue morphology and digestive enzyme activities in digestive tract of lambs. Eighteen healthy newborn male Hu lambs[birth weight of (3.01±0.12) kg] were randomly divided into 3 groups with 6 lambs per group. Lambs in the control group was suckled with ewe, and others in experimental groups were suckled with ewe and fed the starters which NDF levels were 15.13% (high NDF level group) and 10.00% (low NDF level group) at 7 days of age, respectively. All lambs were slaughtered at 35 days of age. The results showed as follows:1) the papilla length and muscular thickness in rumen, the papilla length, papilla width and muscular thickness in reticulum, the papilla width and muscular thickness in omasum, and the muscular thickness in abomasums of high NDF level group were significantly higher than those of the control group (P<0.01), and the papilla length in omasum and muscular thickness in abomasum was significantly higher than those of the control group (P<0.05). The papilla length in rumen and muscular thickness in reticulum of high NDF level group were significantly higher than those of low NDF level group (P<0.01), and the papilla width, muscular thickness in omasum and muscular thickness in abomasum were significantly higher than those of low NDF level group (P<0.05). 2) The crypt depth in duodenum of high and low NDF level group was significantly lower than that of the control group (P<0.01), and the muscular thickness in duodenum of high and low NDF level group was significantly higher than that of the control group (P<0.01). The villus length in jejunum of high NDF level group was significantly higher than that of the control group and low NDF level group (P<0.01), the crypt depth in jejunum of high and low NDF level groups was significantly lower than that of the control group (P<0.01), and the muscular thickness in jejunum of high NDF level group was significantly higher than that of the control group (P<0.05). The villus length in ileum of high and low NDF level groups was significantly higher than that of the control group (P<0.01), and the muscular thickness in ileum of low NDF level group was significantly higher than that of the control group (P<0.01). 3) The rumen protease activity of the control group was significantly higher than that of high and low NDF level groups (P<0.01), and the high NDF level group was significantly higher than that of low NDF level group (P<0.01); the rumen α-amylase activity of low NDF level group was significantly higher than that of the control group (P<0.01). The jejunum α-amylase activity of low NDF level group was significantly higher than that of the control group and high NDF level group (P<0.01), and the activity of trypsin in duodenum, jejunum and ileum of low NDF level group was significantly higher than that of the control group (P<0.01). In conclusion, the 15.13% NDF level of starter is beneficial to the development of digestive tract of lambs before weaning.

Key words： starter; NDF; stomach; small intestine; tissue morphology; digestive enzyme activities

Cite this article

WANG Xiaojuan , LI Fadi , WANG Shiqin , NIAN Fang , LI Chong , WANG Weimin , LIU Ting . Effects of Neutral Detergent Fiber Level of Starter on Tissue Morphology and Digestive Enzyme Activities in Digestive Tract of Lambs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1704 -1712 . DOI: 10.3969/j.issn.1006-267x.2020.04.029

References

[1] 汪晓娟,刘婷,李发弟,等.开食料补饲日龄对湖羊羔羊生长性能和胃发育的影响[J].畜牧兽医学报,2016,47(2):305-314.
[2] 岳喜新,刁其玉,马春晖,等.早期断奶羔羊代乳粉饲喂水平对营养物质消化代谢及血清生化指标的影响[J].中国农业科学,2011,44(21):4464-4473.
[3] YANG B,LE J Q,WU P,et al.Alfalfa intervention alters rumen microbial community development in hu lambs during early life[J].Frontiers in Microbiology,2018,9:574.
[4] DANSO A S,MOREL P C H,KENYON P R,et al.Effect of different feeding regimens on energy and protein utilization and partitioning for maintenance and growth in pre-weaned lambs reared artificially[J].Journal of Animal Science,2016,94(12):5359-5371.

[5] STAMEY J A,JANOVICK N A,KERTZ A F,et al.Influence of starter protein content on growth of dairy calves in an enhanced early nutrition program[J].Journal of Dairy Science,2012,95(6):3327-3336.

[6] QUIGLEY J D,HILL T M,DENNIS T S,et al.Effects of feeding milk replacer at 2 rates with pelleted,low-starch or texturized,high-starch starters on calf performance and digestion[J].Journal of Dairy Science,2018,101(7):5937-5948.

[7] SHEN Z M,SEYFERT H M,LÖHRKE B,et al.An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats[J].The Journal of Nutrition,2004,134(1):11-17.

[8] 祁敏丽,马铁伟,刁其玉,等.饲粮营养限制对断奶湖羊羔羊生长、屠宰性能以及器官发育的影响[J].畜牧兽医学报,2016,47(8):1601-1609.
[9] YANG B,HE B,WANG S S,et al.Early supplementation of starter pellets with alfalfa improves the performance of pre-and postweaning Hu lambs[J].Journal of Animal Science,2015,93(10):4984-4994.

[10] WANG W M,LI C,LI F D,et al.Effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs[J].Scientific Reports,2016,6(1):32479.
[11] LIN L M,XIE F,SUN D M,et al.Ruminal microbiome-host crosstalk stimulates the development of the ruminal epithelium in a lamb model[J].Microbiome,2019,7(1):83.
[12] FRANKLIN S T,AMARAL-PHILLIPS D M,JACKSON J A,et al.Health and performance of Holstein calves that suckled or were hand-fed colostrum and were fed one of three physical forms of starter[J].Journal of Dairy Science,2003,86(6):2145-2153.

[13] FOKKINK W B,HILL T M,BATEMAN II H G,et al.Effect of high-and low-cereal-grain starters on straw intake and rumen development of neonatal Holstein calves[J].Professional Animal Scientist,2011,27(4):357-364.

[14] LANE M A,BALDWIN VI R L,JESSE B W.Sheep rumen metabolic development in response to age and dietary treatments[J].Journal of Animal Science,2000,78(7):1990-1996.

[15] 吕小康,解彪,黄文琴,等.早期饲喂对山羊羔羊瘤胃和小肠组织形态的影响[J].畜牧兽医学报,2019,50(5):1006-1015.
[16] 王珊珊.两种早期断奶补饲模式对羔羊肠道屏障功能发育的影响[D].硕士学位论文.杭州:浙江大学,2015.
[17] 武翠.阿尔巴斯绒山羊在不同饲养条件下小肠形态变化的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2007.
[18] 汪晓娟,刘婷,李发弟,等.开食料补饲日龄对羔羊瘤胃和小肠组织形态的影响[J].草业学报,2016,25(4):172-178.
[19] SUN D M,MAO S Y,ZHU W Y,et al.Effects of starter feeding on caecal mucosal bacterial composition and expression of genes involved in immune and tight junctions in pre-weaned twin lambs[J].Anaerobe,2019,59:167-175.
[20] 中国饲料数据库.中国饲料成分及营养价值表2010年第21版[J].中国饲料,2010(21):34-39.
[21] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6435-2014饲料中水分的测定[S].北京:中国标准出版社,2015.
[22] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018.
[23] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 20806-2006饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社,2007.
[24] 国家市场监督管理总局,中国国家标准化管理委员会.GB/T 6436-2018饲料中钙的测定[S].北京:中国标准出版社,2018.
[25] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6437-2018饲料中总磷的测定分光光度法[S].北京:中国标准出版社,2018.
[26] 杨子江,年芳,李发弟,等.不同精粗比全混合饲粮对肥育羔羊消化道pH和消化酶活性的影响[J].畜牧兽医学报,2009,40(9):1333-1340.
[27] 李建武,余瑞元,袁明秀.生物化学实验原理和方法[M].3版.北京:北京大学出版社,2000.
[28] BRUNO S.酶的测定方法[M].钱嘉渊,译.北京:中国轻工业出版社,1992.
[29] LESMEISTER K E,TOZER P R,HEINRICHS A J.Development and analysis of a rumen tissue sampling procedure[J].Journal of Dairy Science,2004,87(5):1336-1344.

[30] SANDER E G,WARNER R G,HARRISON H N,et al.The stimulatory effect of sodium butyrate and sodium propionate on the development of rumen mucosa in the young calf[J].Journal of Dairy Science,1959,42(9):1600-1605.

[31] SUN D M,MAO S Y,ZHU W Y,et al.Effect of starter diet supplementation on rumen epithelial morphology and expression of genes involved in cell proliferation and metabolism in pre-weaned lambs[J].Animal,2018,12(11):2274-2283.

[32] LIU J,BIAN G,SUN D,et al.Starter feeding altered ruminal epithelial bacterial communities and some key immune-related genes' expression before weaning in lambs[J].Journal of Animal Science,2017,95(2):910-921.
[33] SUÁREZ B J,VAN REENEN C G,STOCKHOFE N,et al.Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves[J].Journal of Dairy Science,2007,90(5):2390-2403.

[34] HARRISON H N,WARNER R G,SANDER E G,et al.Changes in the tissue and volume of the stomachs of calves following the removal of dry feed or consumption of inert bulk[J].Journal of Dairy Science,1960,43(9):1301-1312.

[35] HAMADA T,MAEDA S,KAMEOKA K.Factors influencing growth of rumen,liver,and other organs in kids weaned from milk replacers to solid foods[J].Journal of Dairy Science,1976,59(6):1110-1118.

[36] SUÁREZ B J,VAN REENEN C G,BELDMAN G,et al.Effects of supplementing concentrates differing in carbohydrate composition in veal calf diets:Ⅰ.Animal performance and rumen fermentation characteristics[J].Journal of Dairy Science,2006,89(11):4365-4375.

[37] ÁLVAREZ-RODRÍGUEZ J,MONLEÓN E,SANZ A,et al.Rumen fermentation and histology in light lambs as affected by forage supply and lactation length[J].Research in Veterinary Science,2012,92(2):247-253.

[38] WANG Y H,XU M,WANG F N,et al.Effect of dietary starch on rumen and small intestine morphology and digesta pH in goats[J].Livestock Science,2009,122(1):48-52.

[39] 乔灵,吴美玲.1.5岁阿尔巴斯绒山羊在不同饲养条件下瓣胃黏膜的变化研究[J].黑龙江畜牧兽医,2009(12):32-33.
[40] 马俊南.不同固液比例饲喂模式对犊牛生长及胃肠道发育的影响[D].硕士学位论文.北京:中国农业科学院,2017.
[41] KMET V,BODA K,JAVORSKÝ P,et al.Dynamics of enzymatic activity development in the adherent rumen microflora[J].Archiv für Tierernaehrung,1986,36(7):621-628.

[42] 王宝山.日粮类型对小尾寒羊小肠各段消化酶活性影响的研究[D].硕士学位论文.保定:河北农业大学,2003.
[43] 李佩健.断奶日龄及蛋白质来源对羔羊增重和消化生理的影响[D].硕士学位论文.重庆:西南大学,2009.

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