Effects of Adding Phytase and Protease in Low Phosphorus and Low Fish Meal Diets on Growth Performance and Digestive Physiology of Grass Carp (Ctenopharyngodon idella)

ZHENG Xin; XU Shude; TANG Qifeng; FENG Guohua; AI Qinghui; MAI Kangsen

doi:10.3969/j.issn.1006-267x.2020.04.037

2020 , Vol. 32 >Issue 4: 1788 - 1799

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.037

Effects of Adding Phytase and Protease in Low Phosphorus and Low Fish Meal Diets on Growth Performance and Digestive Physiology of Grass Carp (Ctenopharyngodon idella)

ZHENG Xin ,
XU Shude ,
TANG Qifeng ,
FENG Guohua ,
AI Qinghui ,
MAI Kangsen

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1. Guangdong VTR Bio-Tech Co., Ltd., Zhuhai 519060, China;
2. Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture and Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China;
3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2019-10-18

Online published: 2020-04-16

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Abstract

This experiment was conducted to investigate the effects of adding phytase and protease in low phosphorus (P) and low fish meal (FM) diets on the growth performance, digestive enzyme activities, nutrient apparent digestibility coefficients and immunity of grass carp (Ctenopharyngodon idella). A total of 630 healthy grass carp with an initial body weight about 9.66 g were randomly divided into 7 groups with 3 replicates per group and 30 fish per replicate. Fish in 7 groups were fed the following diets for 56 days:normal P[2.0% Ca(H₂PO₄)₂] and normal FM (3.0% fish meal) diet (control), low P[1.3% Ca(H₂PO₄)₂] and normal FM diet, low P and normal FM+0.03% phytase diet, normal P and low FM (1.5% FM) diet, normal P and low FM+0.05% protease diet, low P and low FM diet, low P and low FM+0.03% phytase+0.05% protease diet. The results showed that compared with the fish fed normal P and normal FM diet, the weight gain rate, specific growth rate, the apparent digestibility coefficients of nutrients[crude protein (CP), calcium (Ca) and P], the activities of digestive enzyme (trypsin, chymotrypsin and amylase) and the activity of serum lysozyme of fish fed low P and low FM diet were significantly decreased (P<0.05), while the feed conversion rate and serum malondialdehyde content were significantly increased (P<0.05). Adding phytase, protease, phytase and protease in the low P and normal FM diet, normal P and low FM and low P and low FM diet, respectively, the above indices of fish were significantly improved (P<0.05). It is concluded that adding phytase and protease in the low P and low FM diet can enhance the nutrient apparent digestibility coefficients, intestinal digestive enzyme actives and immunity, and then improve the growth performance of grass carp.

Key words： low phosphorus; low fish meal; phytase; protease; grass carp; growth performance

Cite this article

ZHENG Xin , XU Shude , TANG Qifeng , FENG Guohua , AI Qinghui , MAI Kangsen . Effects of Adding Phytase and Protease in Low Phosphorus and Low Fish Meal Diets on Growth Performance and Digestive Physiology of Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1788 -1799 . DOI: 10.3969/j.issn.1006-267x.2020.04.037

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