Effects of Dietary Copper Level on Growth, Tissue Copper Accumulation and Serum Biochemical Indexes of Rice Field Eel (Monopterus albus)

LIU Yu; WEI Lili; ZHOU Qiubai; YANG Xin; JIANG Wenhao; HU Chonghua; ZHANG Wenping; WANG Zirui

doi:10.3969/j.issn.1006-267x.2020.04.038

2020 , Vol. 32 >Issue 4: 1800 - 1808

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.038

Effects of Dietary Copper Level on Growth, Tissue Copper Accumulation and Serum Biochemical Indexes of Rice Field Eel (Monopterus albus)

LIU Yu ,
WEI Lili ,
ZHOU Qiubai ,
YANG Xin ,
JIANG Wenhao ,
HU Chonghua ,
ZHANG Wenping ,
WANG Zirui

Expand

College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2019-10-29

Online published: 2020-04-16

Supported by

Fold

Abstract

The dietary copper requirement of rice field eel (Monopterus albus) and the accumulation rule of copper in rice field eel tissues were investigated in this experiment, which provided the basis for the dietary copper level and the food safety of rice field eel. In the present experiment, chalcanthite was used as the copper source, and 0, 10.0, 36.8, 135.7 and 500.0 mg/kg copper were added to basal diet in the form of equal logarithmic interval, respectively. The measured values of copper level in the five diets were 14.21, 23.95, 37.01, 135.63 and 499.63 mg/kg, respectively. The growth indexes, morphological parameters, body composition, tissue copper accumulation amount and serum biochemical indexes of rice field eel with the initial body weight of (62.09±1.07) g were measured after feeding for 60 days under the same aquaculture conditions. Each diet fed to 4 culture nets with 30 rice field eels in each culture net. The results showed as follows:with the dietary copper level increasing, the weight gain rate (WGR) and specific growth rate (SGR) of rice field eel increased firstly and then decreased, while the feed conversion rate (FCR) decreased firstly and then increased. When the dietary copper level was 37.01 mg/kg, the WGR, SGR and FCR were the best. Dietary copper level had significant effects on the copper accumulation amount in the whole fish, liver, intestine, spleen and skin (P<0.05), but had no significant difference effects on the copper accumulation amount in the muscle and kidney (P>0.05), and the muscle copper accumulation amount in each group remained at a low level. The copper accumulation amount in different tissues was in order as follows:liver>intestine>spleen>whole fish>skin, among which the 499.63 mg/kg copper group exceeded the national safety limit of copper in food (50 mg/kg). Dietary copper level had no significant effects on the serum glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (AKP) and lysozyme (LZM) activities (P>0.05). With the dietary copper level increasing, the activities of alanine aminotransferase (GPT), copper-zinc superoxide dismutase (Cu-Zn SOD) and total antioxidant capacity (T-AOC) in serum of rice field eel increased firstly and then maintain stable. The serum GPT activity in 135.63 and 499.63 mg/kg copper groups was significantly higher than that in 14.21, 23.95 and 37.01 mg/kg copper groups (P<0.05), and the serum Cu-Zn SOD activity and T-AOC in 37.01, 135.63 and 499.63 mg/kg copper groups was significantly higher than that in 14.21 and 23.95 mg/kg copper groups (P<0.05). In conclusion, feeding appropriate copper level diet can improve the body antioxidant ability of rice field eel, and insufficient or excessive copper will reduce the growth performance of rice field eel. Copper is mainly accumulated in liver and intestine. Feeding high-copper diet will lead to copper accumulation exceed standard in liver, and there is a risk of liver injury of rice field eel, which has no obvious impact on food safety of its muscle. Using FCR and WGR as evaluation indexes, the copper requirement of 60 to 95 g rice field eel is 44.29 to 45.84 mg/kg.

Key words： rice field eel (Monopterus albus); copper level; growth performance; tissue copper accumulation; serum biochemical indexes

Cite this article

LIU Yu , WEI Lili , ZHOU Qiubai , YANG Xin , JIANG Wenhao , HU Chonghua , ZHANG Wenping , WANG Zirui . Effects of Dietary Copper Level on Growth, Tissue Copper Accumulation and Serum Biochemical Indexes of Rice Field Eel (Monopterus albus)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1800 -1808 . DOI: 10.3969/j.issn.1006-267x.2020.04.038

References

[1] 麦康森.水产动物营养与饲料学[M].2版.北京:中国农业出版社,2011:95.
[2] 常显志,吴志强,黄亮亮,等.漓江5种常见食用经济鱼类重金属含量分析[J].环境科学与技术,2017,40(9):203-208.
[3] 万祖德.叶黄素、氧化豆油、铜和血球蛋白粉对斑点叉尾生长和体色的影响[D].硕士学位论文.上海:上海海洋大学,2016.
[4] LIN Y H,SHIE Y Y,SHIAU S Y.Dietary copper requirements of juvenile grouper,Epinephelus malabaricus[J].Aquaculture,2008,274(1):161-165.

[5] 叶超霞,刘永坚,田丽霞,等.饲料中高水平铜对斜带石斑鱼(Epinephelus coioides)生长和铜、铁、锰、锌含量的影响[J].海洋与湖沼,2013,44(3):606-610.
[6] TAN X Y,LUO Z,LIU X,et al.Dietary copper requirement of juvenile yellow catfish Pelteobagrus fulvidraco[J].Aquaculture Nutrition,2011,17(2):170-176.

[7] SHAO X P,LIU W B,LU K L,et al.Effects of tribasic copper chloride on growth,copper status,antioxidant activities,immune responses and intestinal microflora of blunt snout bream (Megalobrama amblycephala) fed practical diets[J].Aquaculture,2012,338-341:154-159.
[8] CAO J J,MIAO X,XU W,et al.Dietary copper requirements of juvenile large yellow croaker Larimichthys croceus[J].Aquaculture,2014,432:346-350.
[9] BERNTSSEN M H G,LUNDEBYE A K,HAMRE K.Tissue lipid peroxidative responses in Atlantic salmon (Salmo salar L.) parr fed high levels of dietary copper and cadmium[J].Fish Physiology and Biochemistry,2000,23(1):35-48.

[10] CLEARWATER S J,FARAG A M,MEYER J S.Bioavailability and toxicity of dietborne copper and zinc to fish[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2002,132(3):269-313.

[11] 罗鸣钟,斳恒,杨代勤.黄鳝生物学及养殖生态学研究进展[J].水产科学,2014,33(8):529-534.
[12] 闫建林,储张杰,龚世园,等.饲料蛋白质含量对黄鳝生长的影响[J].湖北农业科学,2009,48(1):156-158.
[13] 王松,储张杰,龚世园,等.饲料中脂肪含量对黄鳝生长的影响[J].水利渔业,2008,28(3):67-68.
[14] 王和伟.俄罗斯鲟Acipenser gueldenstaedtii幼鱼对饲料铜的需要和利用研究[D].博士学位论文.上海:华东师范大学,2017:37-38.
[15] MOHSENI M,POURKAZEMI M,BAI S C.Effects of dietary inorganic copper on growth performance and immune responses of juvenile beluga,Huso huso[J].Aquaculture Nutrition,2014,20(5):547-556.

[16] 刘汉超.团头鲂(Megalobrama amblycephala)Fe、Cu、Zn、P需要量的研究[D].硕士学位论文.苏州:苏州大学,2014.
[17] 吴红岩,赵金良,唐首杰,等.华东地区鳜肌肉重金属含量现状与风险分析[J/OL].生态毒理学报,2019:1-15.[2019-12-11].http://kns.cnki.net/kcms/detail/11.5470.X.20190828.1754.002.html.
[18] BAKER R T M,HANDY R D,DAVIES S J,et al.Chronic dietary exposure to copper affects growth,tissue lipid peroxidation,and metal composition of the grey mullet,Chelon labrosus[J].Marine Environmental Research,1998,45(4/5):357-365.
[19] BERNTSSEN M H G,LUNDEBYE A K,MAAGE A.Effects of elevated dietary copper concentrations on growth,feed utilisation and nutritional status of Atlantic salmon (Salmo salar L.) fry[J].Aquaculture,1999,174(1/2):167-181.
[20] 种香玉,周立志,韩冬,等.饲料中铜浓度对异育银鲫和斑点叉尾的影响[J].水生生物学报,2014,38(4):751-763.
[21] KIM S G,KANG J C.Effect of dietary copper exposure on accumulation,growth and hematological parameters of the juvenile rockfish,Sebastes schlegeli[J].Marine Environmental Research,2004,58(1):65-82.

[22] CLEARWATER S J,BASKIN S J,WOOD C M,et al.Gastrointestinal uptake and distribution of copper in rainbow trout[J].Journal of Experimental Biology,2000,203(16):2455-2466.
[23] 刘博,杨文艳,杨连玉.铜肠道稳态平衡调控机制研究进展[J].中国畜牧兽医,2017,44(9):2662-2667.
[24] 蒋蓉.铜、铁、锰、锌对黄颡鱼生长和生理机能的影响[D].硕士学位论文.苏州:苏州大学,2006:58-59.
[25] 崔欣.日粮中高铜对吉富罗非鱼生长、消化及与铜代谢相关生理机能影响的研究[D].硕士学位论文.湛江:广东海洋大学,2013:49.
[26] 王文龙,崔欣,李成成,等.饲料中铜暴露对吉富罗非鱼幼鱼血红细胞微核和组织中铜蓄积的影响[J].生态毒理学报,2014,9(4):757-764.
[27] 中华人民共和国国家质量监督检验检疫总局.GB 18406.4-2001农产品安全质量无公害水产品安全要求[S].北京:中国标准出版社,2004.
[28] 国家水产品质量监督检验中心.NY 5073-2006无公害食品水产品中有毒有害物质限量[S].北京:中国标准出版社,2006.
[29] 刘伟,文华,吴建开,等.高铜饲料对凡纳滨对虾生长、肌肉和肝胰脏铜积累的影响[J].淡水渔业,2008,38(2):12-16.
[30] 唐涛,钟蕾,郇志利,等.3种大豆产品替代鱼粉对黄鳝生长性能、肠道消化酶活性和血清生化指标的影响[J].动物营养学报,2019,31(2):970-980.
[31] AL-AKEL A S,AL-BALAWI H F A,AL-MISNED F,et al.Effects of dietary copper exposure on accumulation,growth,and hematological parameters in Cyprinus carpio[J].Toxicological & Environmental Chemistry,2010,92(10):1865-1878.

[32] 王昌禄,曹俊武,王玉荣,等.铜锌超氧化物歧化酶(SOD)研究进展——从基因到功能[J].现代生物医学进展,2008,8(5):940-943.
[33] 董晓慧,杨原志,郑石轩,等.饲料中不同铜源和水平对凡纳滨对虾生长、免疫和组织铜含量的影响[J].大连水产学院学报,2007(5):377-383.
[34] 孔有琴.日本沼虾(Macrobrachium nipponense)幼虾的铜营养生理研究[D].博士学位论文.上海:华东师范大学,2014:38-39.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References