Effects of High Dose Tannic Acid on Hematological Parameters, Organ Indexes and Histopathology of Weaned Piglets

SONG Yanyan; CHEN Daiwen; YU Bing; HE Jun; LUO Yuheng; MAO Xiangbing; ZHENG Ping; LUO Junqiu; YU Jie

doi:10.3969/j.issn.1006-267x.2020.04.050

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 4: 1899 - 1907

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.050

Effects of High Dose Tannic Acid on Hematological Parameters, Organ Indexes and Histopathology of Weaned Piglets

SONG Yanyan ,
CHEN Daiwen ,
YU Bing ,
HE Jun ,
LUO Yuheng ,
MAO Xiangbing ,
ZHENG Ping ,
LUO Junqiu ,
YU Jie

Expand

Key Laboratory for Animal Disease, Resistance Nutrition of Ministry of Education and Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2019-09-28

Online published: 2020-04-16

Supported by

Fold

Abstract

This experiment was conducted to evaluate the safety of high dose tannic acid (TA) in diet of weaned piglets by studying the effects on hematological parameters, organ indexes and histopathology of weaned piglets. A total of 144 Duroc×Landrace×Yorkshire crossbred weaned piglets (about 21 days old) were allotted to 4 groups (6 replicates per group and each replicate included 3 male and 3 female piglets) in a randomized complete block design according to their gender and body weight. The piglets in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 0.2%, 1.0% and 2.0% TA (extracted from gallnut) to replace the equal amount of soybean meal, respectively. The period of trial was 28 d with 5 d of pretrial. The results showed as follows:compared with the control group, 1) adding 1.0% and 2.0% TA to the diet significantly increased the count of red blood cell in blood (P<0.05), and showed an increasing trend in hemoglobin concentration and hematocrit (0.05 ≤ P < 0.10). In addition, the count of red blood cell, hemoglobin concentration and hematocrit in blood were significantly linearly and positively correlated with the additive dose of TA (P<0.05). 2) The content of albumin in serum had an increasing trend when adding 0.2%, 1.0% and 2.0% TA to the diet (0.05 ≤ P < 0.10). However, the activity of glutamic oxaloacetic transaminase in serum increased linearly with the increase of TA additive dose (P<0.05). 3) Adding 1.0% and 2.0% TA significantly reduced liver index (P<0.05), and the liver index decreased linearly with the increase of TA additive dose (P<0.05); adding 0.2% TA had a decreasing trend to spleen index (0.05 ≤ P < 0.10); adding 0.2% and 1.0% TA had a decreasing trend to kidney index (0.05 ≤ P < 0.10), and the kidney index decreased firstly and then increased with the increase of TA dose, with a secondary regression trend (0.05 ≤ P < 0.10). 4) Adding 0.2%, 1.0% and 2.0% TA to the diet did not cause pathological changes in heart, liver, spleen, lung, kidney, stomach, duodenum, jejunum, ileum, rectum, thymus, pancreas and other tissues of weaned piglets; adding 2.0% of TA resulted in the expansion of the localized lymph node germinal center in the lymphoglandulae mesentericae. It is concluded that dietary supplementation of 0.2%, 1.0% and 2.0% TA reduce the organ indexes of weaned piglets, but have no significant negative impacts on hematological parameters and organ histopathology of weaned piglets. These indicate that adding 2.0% or less TA to the diet is relatively safe for weaned piglets.

Key words： tannic acid; weaned piglets; hematological parameters; organ indexes; histopathology

Cite this article

SONG Yanyan , CHEN Daiwen , YU Bing , HE Jun , LUO Yuheng , MAO Xiangbing , ZHENG Ping , LUO Junqiu , YU Jie . Effects of High Dose Tannic Acid on Hematological Parameters, Organ Indexes and Histopathology of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1899 -1907 . DOI: 10.3969/j.issn.1006-267x.2020.04.050

References

[1] MUELLER-HARVEY I.Unravelling the conundrum of tannins in animal nutrition and health[J].Journal of the Science of Food and Agriculture,2006,86(13):2010-2137.

[2] YE M H,NAN Y L,DING M M,et al.Effects of dietary tannic acid on the growth,hepatic gene expression,and antioxidant enzyme activity in Brandt's voles (Microtus brandti)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2016,196/197:19-26.
[3] GIRARD M,THANNER S,PRADERVAND N,et al.Hydrolysable chestnut tannins for reduction of postweaning diarrhea:efficacy on an experimental ETEC F4 model[J].PLoS One,2018,13(5):e0197878.
[4] JAMROZ D,WILICZKIEWICZ A,SKORUPIŃ SKA J,et al.Effect of sweet chestnut tannin (SCT) on the performance,microbial status of intestine and histological characteristics of intestine wall in chickens[J].British Poultry Science,2009,50(6):687-699.

[5] 刘倩.单宁酸对布氏田鼠代谢的影响[D].硕士学位论文.扬州:扬州大学,2014.
[6] INSERRA L,LUCIANO G,BELLA M,et al.Effect of including carob pulp in the diet of fattening pigs on the fatty acid composition and oxidative stability of pork[J].Meat Science,2015,100:256-261.
[7] CHEN J L,YU B,CHEN D W,et al.Chlorogenic acid improves intestinal barrier functions by suppressing mucosa inflammation and improving antioxidant capacity in weaned pigs[J].The Journal of Nutritional Biochemistry,2018,59:84-92.
[8] HUANG Q Q,LIU X L,ZHAO G Q,et al.Potential and challenges of tannins as an alternative to in-feed antibiotics for farm animal production[J].Animal Nutrition,2018,4(2):137-150.

[9] BARSZCZ M,TACIAK M,SKOMIAŁ J.A dose-response effects of tannic acid and protein on growth performance,caecal fermentation,colon morphology,and β-glucuronidase activity of rats[J].Journal of Animal and Feed Sciences,2011,20(4):613-625.

[10] ČANDEK-POTOKAR M,ŠKRLEP M,LUKA ČN B,et al.Hydrolysable tannin fed to entire male pigs affects intestinal production,tissue deposition and hepatic clearance of skatole[J].The Veterinary Journal,2015,204(2):162-167.

[11] BIAGI G,CIPOLLINI I,PAULICKS B R,et al.Effect of tannins on growth performance and intestinal ecosystem in weaned piglets[J].Archives of Animal Nutrition,2010,64(2):121-135.

[12] SCHIAVONE A,GUO K,TASSONE S,et al.Effects of a natural extract of chestnut wood on digestibility,performance traits,and nitrogen balance of broiler chicks[J].Poultry Science,2008,87(3):521-527.

[13] 王黎琦,汪攀,曹珺,等.约氏乳杆菌对断奶仔猪生产性能、血液学指标、脏器指数和组织病理学的影响[J].中国畜牧杂志,2016,52(21):76-80.
[14] 向发.五倍子溶液剂的安全性评价[D].硕士学位论文.雅安:四川农业大学,2015.
[15] LEE S H,SHINDE P L,CHOI J Y,et al.Effects of tannic acid supplementation on growth performance,blood hematology,iron status and faecal microflora in weanling pigs[J].Livestock Science,2010,131(2/3):281-286.
[16] GOORDEN S M I,BUFFART T E,BAKKER A,et al.Liver disorders in adults:ALT and AST[J].Nederlands Tijdschrift Voor Geneeskunde,2013,157(43):A6443.
[17] 刘英娜,张慧媛,林嘉楠,等.单宁酸对脂多糖诱导小鼠肝损伤的保护作用及机制研究[J].人参研究,2017(6):36-39.
[18] 盖向荣,何东亭,杨文军,等.高剂量乙氧基喹啉对生长育肥猪生长性能、血清生化指标、抗氧化性能、脏器指数和肉品质的影响[J].动物营养学报,2018,30(9):3643-3651.
[19] MEBIUS R E,KRAAL G.Structure and function of the spleen[J].Nature Reviews Immunology,2005,5(8):606-616.

[20] 朱美霖.单宁对布氏田鼠(Lasiopodomys brandtii)食物选择的影响[D].硕士学位论文.郑州:郑州大学,2017.
[21] 廖子傑,严意华,胡雪峰.生发中心的发生与发展[J].中国生物化学与分子生物学报,2018,34(6):610-616.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References