Effects of Different Roughage Combinations on Fermentation Parameters of Distiller's Drains Diet in Vitro

ZHOU Xinyu; WANG Zhisheng; ZHU Yixiao; DAI Qindan; PENG Quanhui; ZOU Huawei; XUE Bai; WANG Lizhi

doi:10.3969/j.issn.1006-267x.2020.04.054

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 4: 1936 - 1945

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.04.054

Effects of Different Roughage Combinations on Fermentation Parameters of Distiller's Drains Diet in Vitro

ZHOU Xinyu ,
WANG Zhisheng ,
ZHU Yixiao ,
DAI Qindan ,
PENG Quanhui ,
ZOU Huawei ,
XUE Bai ,
WANG Lizhi

Expand

Key Laboratory of Low Carbon Culture and Safety Production in Cattle in Sichuan, Animal Nutrition Institute of Sichuan Agriculture University, Chengdu 611130, China

Received date: 2019-10-17

Online published: 2020-04-16

Supported by

Fold

Abstract

The purpose of this experiment was to investigate the combination effects of different roughages and the effects of the best combination selected and the fixed proportion of concentrate on fermentation parameters in vitro. Maotai flavor fermented distiller's grains, Luzhou flavor dried distiller's grains, Luzhou flavor wet distiller's grains and Maotai flavor were mixed with rice straw and oat hay at the ratios of 0:100, 25:75, 50:50, 75:25 and 100:0, respectively. In vitro fermentation indexes[gas production, pH, microbial proteins (MCP) and single-factors associative effects index (SFAEI) of 48 h gas production] were analyzed by in vitro gas production technique. According to SFAEI, the best combinations of four distiller's grains with rice straw and oat hay was mixed with concentrate (6:4 ratio of concentrate to roughage) and analyzed by in vitro gas production technique. The results showed as follows:1) the combination of four distiller's grains with two kinds of hay could produce positive combination effect, and the combination effect with oat hay was higher than that with rice straw. 2) The best combination ratio and SFAEI were:Maotai flavor fermented distiller's grains:oat hay=25:75, SFAEI=11.48; Luzhou flavor dried distiller's grains:oat hay=25:75, SFAEI=3.95; Luzhou flavor wet distiller's grains:oat hay=50:50, SFAEI=6.70; Maotai flavor wet distiller's grains:oat hay=25:75, SFAEI=6.47. 3) Under the same proportion of diets, methane production of Maotai flavor fermented disriller's grains group (60% concentrate+10% Maotai flavor fermented distiller's grains+30% oat hay) was lower than that of Luzhou flavor wet distiller's grains group (60% concentrate+20% Luzhou flavor wet distiller's grains+20% oat hay) and Maotai flavor wet distiller's grains group (60% concentrate+10% Maotai flavor wet distiller's grians+30% oat hay) (P<0.05). The carbon dioxide production of Maotai flavor fermented distiller's grains group and Luzhou flavor dried dstiller's grains group (60% concentrate+10% Luzhou flavor dried distiller's grains+30% oat hay) was also lower than that of the other two wet distiller's grains group (P<0.05), while volatile fatty acid contents were higher than those of two wet distiller's grains group (P<0.05). In conclusion, according to SFAEI, the best combination of Maotai flavor fermented distiller's grains, Luzhou flavor dried distiller's grains, Maotai flavor wet distiller's grains with oat hay is 25:75, and the best combination of Luzhou flavor wet distiller's grains with oat hay is 50:50. Maotai flavor fermented distiller's grains group and Luzho flavor dried distiller's grains group diet have better fermentation effect in vitro.

Key words： roughage combinations; distiller's grains diet; in vitro fermentation parameters

Cite this article

ZHOU Xinyu , WANG Zhisheng , ZHU Yixiao , DAI Qindan , PENG Quanhui , ZOU Huawei , XUE Bai , WANG Lizhi . Effects of Different Roughage Combinations on Fermentation Parameters of Distiller's Drains Diet in Vitro[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1936 -1945 . DOI: 10.3969/j.issn.1006-267x.2020.04.054

References

[1] 江源.《特香型白酒》等60项标准4月起实施[J].酿酒科技,2018(5):101.
[2] FREITAS T B,RELLING A E,PEDREIRA M S,et al.Effects of sodium hydroxide treatment of dried distillers' grains on digestibility,ruminal metabolism,and metabolic acidosis of feedlot steers[J].Journal of Animal Science,2015,94(2):709-717.
[3] POKHAREL S.Effects of feeding high levels of wet distillers grains and straw on beef quality[D].Master's thesis.California:University of Nebraska,2011.
[4] KUNG L,Jr.The role of fiber in ruminant ration formulation[D].Ph.D. thesis.Delaware:Univercity of Delaware,2014.
[5] 中华人民共和国国家统计局.中国统计年鉴[M].北京:中国统计出版社,2018:405-408.
[6] JACOB M E,PADDOCK Z D,RENTER D G,et al.Inclusion of dried or wet Distillers' grains at different levels in diets of feedlot cattle affects fecal shedding of Escherichia coli O157:H7[J].Applied and Environmental Microbiology,2010,76(21):7238-7242.

[7] SUN P F,WU Y M,LIU J X.In vitro gas production technique to evaluate associative effects among lucerne hay,rice straw and maize silage[J].Journal of Animal and Feed Sciences,2007,16(2):272-277.
[8] LIU J X,SUSENBETH A,SVDEKUM K H.In vitro gas production measurements to evaluate interactions between untreated and chemically treated rice straws,grass hay,and mulberry leaves[J].Journal of Animal Science,2002,80(2):517-524.

[9] 崔占鸿,郝力壮,刘书杰,等.体外产气法评价青海高原燕麦青干草与天然牧草组合效应[J].草业学报,2012,21(3):250-257.
[10] 林奕.白酒糟、玉米和豆粕之间组合效应及其对瘤胃发酵特性影响的研究[D].硕士学位论文.雅安:四川农业大学,2009.
[11] GILL R K,VANOVERBEKE D L,DEPENBUSCH B,et al.Impact of beef cattle diets containing corn or sorghum distillers grains on beef color,fatty acid profiles,and sensory attributes[J].Journal of Animal Science,2008,86(4):923-935.

[12] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[13] MENKE K H,RAAB L,SALEWSKI A,et al.The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J].The Journal of Agriculture Science,1979,93(1):217-222.

[14] 庄二林.苜蓿与谷草组合效应的评定研究[D].硕士学位论文.杨凌:西北农林科技大学,2017.
[15] BRODERICK C A,KANG J H.Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro medis[J].Journal of Dairy Science,1980,63(1):64-75.

[16] BLVMMEL M,MAKKAR H P S,BECKER K.In vitro gas production:a technique revisited[J].Journal of Animal Physiology and Animal Nutrition,1997,77(1/2/3/4/5):24-34.
[17] 张毕阳,赵桂琴,焦婷,等.饲粮中添加燕麦干草对绵羊体外发酵的影响[J].草业学报,2018,27(2):182-191.
[18] 李倩,裴朝曦,王之盛,等.不同类型酒糟营养成分组成差异的比较研究[J].动物营养学报,2018,30(6):2369-2376.
[19] CONE J W,VAN GELDER A H,BACHMANN H.Influence of inoculum source on gas production profiles[J].Animal Feed Science and Technology,2002,99(1/2/3/4):221-231.
[20] 张一帆,李妍,刘涛,等.玉米秸秆青贮、羊草、燕麦草与精饲料组合效应的研究[J].动物营养学报,2019,31(8):3853-3862.
[21] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
[22] 望丕县,潘林阳,吴正荣,等.饲料学[M].北京:北京农业大学出版社,1991.
[23] NOCEK J E,RUSSELL J B.Protein and energy as an integrated system.Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production[J].Journal of Dairy Science,1988,71(8):2070-2107.

[24] SU J Z,CHAUDHRY A S,OSMAN A,et al.Associative effects of ensiling mixtures of sweet sorghum and alfalfa on nutritive value,fermentation and methane characteristics[J].Animal Feed Science and Technology,2015,206:29-38.
[25] CATON J S,DHUYVETTER D V.Influence of energy supplementation on grazing ruminants:requirements and responses[J].Journal of Animal Science,1997,75(2):533-542.

[26] MACCARANA L,CATTANI M,BAILONI L.Rumen in vitro gas production of combinations between slowly and rapidly fermentable fibre sources[J].Agriculturae Conspectus Scientificus,2013,78(3):229-233.
[27] MOE P W,TYRRELL H F.Methane production in dairy cows[J].Journal of Dairy Science,1979,62(6):1583-1586.
[28] 娜仁花.不同日粮对奶牛瘤胃甲烷及氮排放的影响研究[D].博士学位论文.北京:中国农业科学院,2010.
[29] 李建国.规模化生态奶牛养殖技术[M].北京:中国农业大学出版社,2013.
[30] ILLIUS A W.Matching ruminant production systems with available resources in the tropics and sub-tropics[J].Agricultural Systems,1989,30(2):200-201.

[31] 曾燕霞,陈志龙,王林,赵臣,郑琛.不同精粗比日粮中添加甘露寡糖对绵羊体外发酵的影响[J].中国畜牧兽医,2016,43(1):68-75.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References