Effects of Combined Exposure of Zearalenone and Deoxynivalenol on Reproductive Toxicity of Male Mice and Alleviation of Tea Polyphenols

  • CAO Li ,
  • ZHAO Jie ,
  • CHEN Lingxia ,
  • XIE Xin ,
  • RAN Meng ,
  • SHEN Maoyu ,
  • ZHU Lei ,
  • FENG Shibin ,
  • LI Yu ,
  • WU Jinjie ,
  • WANG Xichun
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  • College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2019-09-30

  Online published: 2020-04-16

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Abstract

The present experiment was conducted to evaluate the alleviation of tea polyphenols on reproductive toxicity injury of mice induced by zearalenone (ZEN) and deoxynivalenol (DON). Seventy-five 8-week-old Kunming (male) mice were randomly divided into 5 groups, and each group was administered saline (control group), 200 mg/kg tea polyphenols (group Ⅰ), 1 mg/kg ZEN+0.5 mg/kg DON (group Ⅱ), 100 mg/kg tea polyphenols+1 mg/kg ZEN+0.5 mg/kg DON (group Ⅲ) and 200 mg/kg tea polyphenols+1 mg/kg ZEN+0.5 mg/kg DON (group Ⅳ), respectively, 15 mice in each group. The experiment lasted for 28 d. On the days 1, 14 and 28, five mice in each group were randomly selected and weighed, the sperm quality, testicular organ coefficient, testicular tissue marker enzymes activities, serum sex hormone contents, testicular tissue oxidation and antioxidant indexes of mice were detected, and testicular tissue sections were prepared. The results showed as follows:1) compared with the control group, testicular organ coefficient, sperm survival rate and quantity, serum sex hormone contents of mice in group Ⅱ were decreased significantly (P<0.05), the activities of alkaline phosphatase (AKP), lactate dehydrogenase (LDH), γ-glutamyltransferase (γ-GGT), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in testicular tissue were decreased significantly (P<0.05) and the sperm abnormality rate and the content of malondialdehyde (MAD) in serum increased significantly (P<0.05). After adding tea polyphenols, testicular organ coefficient, sperm survival rate and quantity, serum sex hormone contents, the activities of AKP, LDH, γ-GGT, CAT, SOD and GSH-Px in testicular tissue were increased significantly (P<0.05), the sperm abnormality rate and the content of MDA in serum were decreased significantly (P<0.05). Over time, compared with day 1, the activity of GSH-Px in testicular tissue in group Ⅰ was significantly increased (P<0.05), the testicular organ coefficient, sperm quality, testicular tissue marker enzymes activities, serum sex hormone contents, testicular tissue oxidation and antioxidant indexes in group Ⅱ were significantly changed (P<0.05), the activities of CAT and GSH-Px in testicular tissue in group Ⅲ were significantly decreased (P<0.05), the activity of CAT in testicular tissue in group Ⅳ was significantly decreased (P<0.05). 2) Histopathological sections of testis showed that the basement membrane of spermatogenic tubules was incomplete, cells arranged disorderly, the number and volume of spermatogenic tubules decreased, vacuolation between cells and sperm quantity decreased in the testicular lumen of mice in the exposed group, and improvement after protection by tea polyphenols. These results suggest that tea polyphenols can effectively improve the quality of sperm and the activities of marker enzymes in testicular tissues, the contnets of serum sex hormones and the antioxidant function of testicular tissues, decrease the reproductive toxicity of mice induced by ZEN and DON.

Cite this article

CAO Li , ZHAO Jie , CHEN Lingxia , XIE Xin , RAN Meng , SHEN Maoyu , ZHU Lei , FENG Shibin , LI Yu , WU Jinjie , WANG Xichun . Effects of Combined Exposure of Zearalenone and Deoxynivalenol on Reproductive Toxicity of Male Mice and Alleviation of Tea Polyphenols[J]. Chinese Journal of Animal Nutrition, 2020 , 32(4) : 1946 -1956 . DOI: 10.3969/j.issn.1006-267x.2020.04.055

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