SWINE NUTRITION AND FEED

Glutamate Enhances Porcine Ileal Barrier Function via Protecting Ileal Structural Integrality

  • QIN Yingchao ,
  • SONG Zhiwen ,
  • ZHU Min ,
  • GAO Chunqi ,
  • YAN Huichao ,
  • WANG Xiuqi
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  • National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2019-11-08

  Online published: 2020-05-15

Abstract

This study investigated the effects of glutamate on ileal structure and barrier function of weaned piglets by adding glutamate into their diets. Fourteen Duroc×Landrace×Yorkshire crossbred piglets with the similar body weight[(7.90±0.50) kg] at the age of 28 days were selected and randomly divided into control group and glutamate group, with 7 replicates in each group and one pig in each replicate. The diet of control group was a corn-soybean meal type based diet supplemented with 1.0% corn starch, while the diet of glutamate group was the corn-soybean type basal diet supplemented with 1.0% glutamate. The experimental period was 28 days, including 7 days of pre-feeding period and 21 days of trial period. The results showed as follows:compared with the control group, dietary supplemented with 1.0% glutamate could significantly increase ileal weight per unit length and mucosal weight per unit length of weaned piglets (P<0.05); significantly increase the ileal villi height and villi height/crypt depth (P<0.05), and the ileal morphology was more complete; significantly increase the relative expression levels of proteins related to tight junction (occludin and claudin-1) and proliferative marker protein[proliferating cell nuclear antigen (PCNA)] of ileal epithelial cells (P<0.05); significantly reduce the serum lipopolysaccharide content and diamine oxidase activity (P<0.05); significantly up-regulate the expression of proteins related to JAK2/STAT3 signaling pathway in ileum tissue (P<0.05). The results suggest that dietary supplementation with 1.0% glutamate can promote ileal epithelial cell proliferation by up-regulating the expression of proteins related to JAK2/STAT3 signaling pathway in ileum tissue, thus increasing barrier function and protecting the structural integrity of the ileum of weaned piglets.

Cite this article

QIN Yingchao , SONG Zhiwen , ZHU Min , GAO Chunqi , YAN Huichao , WANG Xiuqi . Glutamate Enhances Porcine Ileal Barrier Function via Protecting Ileal Structural Integrality[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2101 -2107 . DOI: 10.3969/j.issn.1006-267x.2020.05.018

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