Assessment on Detoxification Effects of Mycotoxin Adsorbent on Zearalenone

YANG Fan; ZHANG Junnan; WANG Jinquan; LYU Zonghao; LIU Jei; ZHENG Yunduo

doi:10.3969/j.issn.1006-267x.2020.05.020

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 5: 2116 - 2125

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.020

SWINE NUTRITION AND FEED

Assessment on Detoxification Effects of Mycotoxin Adsorbent on Zearalenone

YANG Fan ,
ZHANG Junnan ,
WANG Jinquan ,
LYU Zonghao ,
LIU Jei ,
ZHENG Yunduo

Expand

Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2019-11-03

Online published: 2020-05-15

Fold

Abstract

This study was conducted to assess the in vitro and in vivo detoxification effects of different mycotoxin absorbents on zearalenone (ZEA). In vitro binding efficacy of ZEA by twelve different mycotoxin absorbents in buffer solutions with pH 3.0 and 6.5 was analyzed and compared. The absorbents A3, A4 and A6 with the highest in vitro binding efficacy were selected for in vivo evaluation. Forty healthy prepubertal gilts (Duroc×Landrace×Large white) were allocated into 5 groups according to age[(75±2) days of age] and average body weight[(29.19±0.76) kg] (8 replicates per group and 1 pig per replicate). Prepubertal gilts in control group and ZEA group were fed a basal diet supplemented with 0 and 1 mg/kg ZEA, respectively. The experimental groups (E1, E2 and E3) were fed the ZEA group diet supplemented with 0.2% (w/w) different absorbents (A3, A4 and A6). The adaptation period lasted for 7 days and the trial period lasted for 21 days. The in vivo detoxification effect of adsorbents was evaluated by comparing the growth performance, vulva width, organ indexes, serum biochemical indexes and the contents of ZEA and its metabolites in plasma among different groups. The results showed as follows:1) in vitro adsorption test revealed that 12 kinds of adsorbents had different adsorption capacity in buffer solutions with different pH, of which A3, A4 and A6 had the highest binding efficacy on ZEA, and the adsorption rates reached more than 90% in both media with pH 3.0 and 6.5. 2) Compared with control group, average daily feed intake, average daily gain, feed to gain ratio, liver index, spleen index, kidney index, the contents of total protein, albumin, immunoglobulin G and the activities of alkaline phosphatase, glutamate dehydrogenase, glutamic oxalacetic transaminase, glutamyltranspeptidase and creatine jubase in serum of prepubertal gilts were not significantly affected in ZEA group with the addition of 1 mg/kg ZEA (P>0.05). However, vulva width, reproductive organ index and the contents of ZEA, α-zearalanel (ZOL) and β-ZOL in plasma were significantly increased (P<0.05), while the estradiol content in serum was significantly decreased (P<0.05). 3) The addition of 0.2% absorbent A4 or A6 in ZEA diet could recover the reproductive organ index to the level of control group. All the three adsorbents recovered the estradiol content in serum to some degree. Adsorbent A4 could significantly decreased vulva width, the contents of ZEA and its metabolites in plasma (P<0.05). It is concluded that different mycotoxin absorbents has different adsorption capacity on ZEA in vitro, and the in vitro adsorption capacity cannot completely represent the in vivo detoxification effect. The evaluation of the detoxification effect of mycotoxin adsorbents requires the combination of in vivo and in vitro trials.

Key words： absorbent; zearalenone; detoxification effect; in vitro evaluation; in vivo evaluation; biomarker

Cite this article

YANG Fan , ZHANG Junnan , WANG Jinquan , LYU Zonghao , LIU Jei , ZHENG Yunduo . Assessment on Detoxification Effects of Mycotoxin Adsorbent on Zearalenone[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2116 -2125 . DOI: 10.3969/j.issn.1006-267x.2020.05.020

References

[1] ZINEDINE A,SORIANO J M,MOLTÓ J C,et al.Review on the toxicity,occurrence,metabolism,detoxification,regulations and intake of zearalenone:an oestrogenic mycotoxin[J].Food and Chemical Toxicology,2007,45(1):1-18.

[2] SHI H T,LI S L,BAI Y Y,et al.Mycotoxin contamination of food and feed in China:occurrence,detection techniques,toxicological effects and advances in mitigation technologies[J].Food Control,2018,91:202-215.
[3] 何学军,齐德生.玉米赤霉烯酮的毒性研究进展[J].中国饲料,2006(10):2-5.
[4] EFSA.Review of mycotoxin-detoxifying agents used as feed additives:mode of action,efficacy and feed/food safety[EB/OL].(2014-08-29).http://www.efsa.europa.eu/en/scdocs/scdoc/22e.htm.
[5] PHILLIPS T D,KUBENA L F,HARVEY R B,et al.Hydrated sodium calcium aluminosilicate:a high affinity sorbent for aflatoxin[J].Poultry Science,1988,67(2):243-247.

[6] 安亚南,王丹阳,丁立人,等.猪胃肠道食糜中黄曲霉毒素B₁和玉米赤霉烯酮检测方法的改进及其在霉菌毒素吸附剂吸附效果评价中的应用[J].动物营养学报,2015,27(6):1823-1831.
[7] 姚宝强.玉米赤霉烯酮和吸附剂对断奶仔猪生殖发育、血液生化指标和组织形态学的影响[D].硕士学位论文.泰安:山东农业大学,2009.
[8] 姜淑贞.玉米赤霉烯酮对断奶仔猪的毒性初探及改性蒙脱石的脱毒效应研究[D].博士学位论文.泰安:山东农业大学,2010.
[9] EFSA Panel on Additives and Products or Substances Used in Animal Feed (FEEDAP).Statement on the establishment of guidelines for the assessment of additives from the functional group ‘substances for reduction of the contamination of feed by mycotoxins’[J].EFSA Journal,2010,8(7):1693.
[10] MALEKINEJAD H,MAAS-BAKKER R,FINK-GREMMELS J.Species differences in the hepatic biotransformation of zearalenone[J].Veterinary Journal,2006,172(1):96-102.

[11] 罗丹,廖祉亦,温超,等.凹凸棒石吸附剂对蛋鸡生产性能、血清生化指标、抗氧化功能及玉米赤霉烯酮残留的影响[J].中国家禽,2018,40(20):28-32.
[12] 杨立杰.玉米赤霉烯酮对断奶仔猪免疫毒性及生殖毒性的影响[D].硕士学位论文.泰安:山东农业大学,2018.
[13] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:48-96.
[14] 王瑞国,苏晓鸥,王培龙,等.液相色谱-串联质谱法同时测定动物血浆中21种霉菌毒素或其代谢物残留[J].分析化学,2017,45(2):231-237.
[15] DEVREESE M,ANTONISSEN G,DE BACKER P,et al.Efficacy of active carbon towards the absorption of deoxynivalenol in pigs[J].Toxins,2014,6(10):2998-3004.

[16] 王瑞国,苏晓鸥,樊霞,等.液相色谱-串联质谱法测定人工模拟猪消化液中3种霉菌毒素[J].分析化学,2015,43(1):1-6.
[17] LEMKE S L,GRANT P G,PHILLIPS T D.Adsorption of zearalenone by organophilic montmorillonite clay[J].Journal of Agricultural and Food Chemistry,1998,46(9):3789-3796.

[18] 姚志成,叶盛群,许家亮,等.改性蒙脱石对霉菌毒素体外吸附脱毒效果试验[J].山东畜牧兽医,2012(9):19-20.
[19] 张丽霞,徐学明.啤酒酵母β-D-葡聚糖吸附毒素玉米赤霉烯酮(ZEA)的研究[J].食品科学,2006,27(4):75-78.
[20] 许静.玉米赤霉烯酮脱毒剂的筛选及其脱毒机制初步研究[D].硕士学位论文.武汉:华中农业大学,2014.
[21] MARCELA Š,LIKER B,ŠPERANDA T,et al.Haematological and biochemical parameters of weaned piglets fed on fodder mixture contaminated by zearalenone with addition of clinoptilolite[J].Acta Veterinaria,2006,56(2):121-136.
[22] 赵虎.玉米赤霉烯酮对断奶仔猪生产性能、免疫功能和器官发育影响的研究[D].硕士学位论文.泰安:山东农业大学,2008.
[23] 王定发.玉米赤霉烯酮联合大豆异黄酮对青年母猪生殖器官发育、肝脏损伤和组织玉米赤霉烯酮残留的影响及其机制研究[D].博士学位论文.武汉:华中农业大学,2011.
[24] UNDERHILL K L,ROTTER B A,THOMPSON B K,et al.Effectiveness of cholestyramine in the detoxification of zearalenone as determined in mice[J].Bulletin of Environmental Contamination and Toxicology,1995,54(1):128-134.
[25] ABBÈS S,OUANES Z,BEN SALAH-ABBẼS J,et al.The protective effect of hydrated sodium calcium aluminosilicate against haematological,biochemical and pathological changes induced by zearalenone in mice[J].Toxicon,2006,47(5):567-574.

[26] 王慧容.三种霉菌毒素吸附剂对复合霉菌毒素中毒肉鸡解毒效果的研究[D].硕士学位论文.武汉:武汉工业学院,2008.
[27] National Toxicology Program.Carcinogenesis bioassay of zearalenone (CAS No.17924-92-4) in F344/N rats and B6C3F1 mice (feed study)[J].National Toxicology Program Technical Report,1982,235:1-155.
[28] LEBLANC G A,BAIN L J,WILSON V S.Pesticides:multiple mechanisms of demasculinization[J].Molecular and Cellular Endocrinology,1997,126(1):1-5.

[29] SONGSERMSAKUL P,BÖHM J,AURICH C,et al.The levels of zearalenone and its metabolites in plasma,urine and faeces of horses fed with naturally,Fusarium toxin-contaminated oats[J].Journal of Animal Physiology and Animal Nutrition,2013,97(1):155-161.

[30] VIDAL A,MENGELERS M,YANG S P,et al.Mycotoxin biomarkers of exposure:a comprehensive review[J].Comprehensive Reviews in Food Science and Food Safety,2018,17(5):1127-1155.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References