Effects of Exogenous Melatonin on Performance, Serum Indexes and Melatonin Content of Laying Hens during Later Laying Period

HAO Erying; HUANG Chenxuan; SUN Haozheng; WANG Dehe; ZHOU Rongyan; HUANG Renlu; CHEN Hui

doi:10.3969/j.issn.1006-267x.2020.05.021

2020 , Vol. 32 >Issue 5: 2126 - 2137

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.021

POULTRY NUTRITION AND FEED

Effects of Exogenous Melatonin on Performance, Serum Indexes and Melatonin Content of Laying Hens during Later Laying Period

HAO Erying ,
HUANG Chenxuan ,
SUN Haozheng ,
WANG Dehe ,
ZHOU Rongyan ,
HUANG Renlu ,
CHEN Hui

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College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China

Received date: 2019-10-29

Online published: 2020-05-15

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Abstract

To investigate the effects of different concentrations of exogenous melatonin on performance, melatonin content and serum indexes of laying hens during later laying period, 60 healthy Dawu Jinfeng laying hens with similar healthy body weight were randomly divided into 3 groups, each group had 4 repetitions, and each had 5 repetitions. The control group was intraperitoneally injected normal saline. The test group Ⅰ was injected intraperitoneally with 20 mg/(kg·d) melatonin, and the test group Ⅱ was intraperitoneally injected with 50 mg/(kg·d) melatonin. The illumination condition was 16L:8D, the test period included a 7-d pre-test period and a 28-d trial period. The results showed as follows:1) compared with the control group, the melatonin treatment significantly increased the laying rate at d 15 to 21 and d 22 to 28 (P<0.01), and significantly increased the number of large white follicles (P<0.05), but had no significant effect on egg quality (P>0.05). 2) Compared with the control group, the melatonin treatment significantly increased the melatonin contents in the serum and yolk at d 21 and 28 (P<0.05 or P<0.01); at d 28, the 50 mg/(kg·d) melatonin treatment significantly increased the melatonin contnet in the ovaries (P<0.01). 3) Compared with the control group, at d 21 and 28, 50 mg/(kg·d) melatonin significantly increased the superoxide dismutase (SOD) activity and total antioxidant capacity (T-AOC) (P<0.01), and significantly reduced the content of oxygen free radicals (ROS) (P<0.01); at d 28, 50 mg/(kg·d) melatonin significantly increased the activity of SOD and T-AOC (P<0.01), and significantly reduced the content of ROS in the ovary (P<0.01). 4) Compared with the control group, at d 21 and 28, 50 mg/(kg·d) melatonin significantly increased the serum immunoglobulin (Ig) A, IgG and IgM contents (P<0.01). 5) Compared with the control group, melatonin treatment significantly increased the follicle-stimulating hormone (FSH) and luteotropin (LH) contents in serum at d 21 and 28 (P<0.01). Exogenous melatonin treatment significantly improves the laying rate of laying hens in the later laying period, improves the body's antioxidant performance, immune performance, and reproductive hormone levels. It provided a theoretical basis for exogenous melatonin supplementation to improve egg production during the end of laying.

Key words： melatonin; later laying period; laying rate; antioxidant index; number of follicles

Cite this article

HAO Erying , HUANG Chenxuan , SUN Haozheng , WANG Dehe , ZHOU Rongyan , HUANG Renlu , CHEN Hui . Effects of Exogenous Melatonin on Performance, Serum Indexes and Melatonin Content of Laying Hens during Later Laying Period[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2126 -2137 . DOI: 10.3969/j.issn.1006-267x.2020.05.021

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