POULTRY NUTRITION AND FEED

Effects of Bacillus coagulans on Growth Performance and Serum Indices of Broiler Chickens under Heat Stress

  • WANG Yiru ,
  • LIU Chengdong ,
  • BAI Huayi ,
  • QU E ,
  • ZHAO Ping ,
  • LI Qihua ,
  • PHOUTHAPANE Vanhnaseng ,
  • CHENG Zhibin
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  • 1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
    2. Biotechnology and Ecology Institute, Ministry of Science and Technology, Vientiane 2279, Laos

Received date: 2019-11-12

  Online published: 2020-05-15

Abstract

This experiment was conducted to study the effects of Bacillus coagulans on growth performance and serum indices of broiler chickens under heat stress. Two hundred and forty 28-day-old healthy male Arbor Acres broiler chickens were randomly divided into 3 groups with 8 replicates per group and 10 broilers per replicate. Broiler chickens in normal temperature group (group Ⅰ) were raised in the normal temperature environment of (22±1)℃, and fed a basal diet; broiler chickens in heat stress group (group Ⅱ) were raised in an extremely high temperature environment of (35±1)℃, and fed a basal diet; broiler chickens in heat stress+Bacillus coagulans group (group Ⅲ) were raised in an extremely high temperature environment of (35±1)℃, and fed the basal diet+1.3 kg/t Bacillus coagulans. The experiment lasted for 14 days. The results showed that compared with group Ⅰ, the feed to gain ratio, rectal temperature and contents of corticosterone, uric acid (UA), low density lipoprotein cholesterol (LDL-C) and interferon-γ (IFN-γ) in serum of groups Ⅱ and Ⅲ were significantly increased (P<0.05), while the final body weight, average daily gain, average daily intake and contents of total protein (TP), albumin (ALB), high density lipoprotein-cholesterol (HDL-C), interleukin-10 (IL-10) and catalase (CAT) activity in serum were significantly decreased (P<0.05). Compared with group Ⅱ, the feed to gain ratio and contents of corticosterone, UA, triglyceride (TG), LDL-C, malondialdehyde (MDA) and IFN-γ, as well as the activities of creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyl transferase (GGT) in serum of group Ⅲ were significantly decreased (P<0.05), while the average daily gain and CAT activity and IL-10 content in serum were significantly increased (P<0.05). In conclusion, dietary supplementation of Bacillus coagulans can reduce the oxidative damage and inflammation response, and partly improve the growth performance of broiler chickens under heat stress.

Cite this article

WANG Yiru , LIU Chengdong , BAI Huayi , QU E , ZHAO Ping , LI Qihua , PHOUTHAPANE Vanhnaseng , CHENG Zhibin . Effects of Bacillus coagulans on Growth Performance and Serum Indices of Broiler Chickens under Heat Stress[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2148 -2157 . DOI: 10.3969/j.issn.1006-267x.2020.05.023

References

[1] WAN X L,JIANG L Y,ZHONG H R,et al.Effects of enzymatically treated Artemisia annua L. on growth performance and some blood parameters of broilers exposed to heat stress[J].Animal Science Journal,2017,88(8):1239-1246.  
[2] NAWAB A,IBTISHAM F,LI G H,et al.Heat stress in poultry production:mitigation strategies to overcome the future challenges facing the global poultry industry[J].Journal of Thermal Biology,2018,78:131-139.
[3] HU R Z,HE Y J,AROWOLO M,et al.Polyphenols as potential attenuators of heat stress in poultry production[J].Antioxidants,2019,8(3):67.
[4] FARAG M R,ALAGAWANY M.Physiological alterations of poultry to the high environmental temperature[J].Journal of Thermal Biology,2018,76:101-106.
[5] 葛长荣,程志斌.饲用微生态制剂对热应激肉鸡生产性能影响的研究进展[J].饲料工业,2019,40(3):1-9.
[6] PAWAR S S,BASAVARAJ S,DHANSING L V,et al.Assessing and mitigating the impact of heat stress in poultry[J].Advances in Animal and Veterinary Sciences,2016,4(6):332-341.  
[7] SUGIHARTO S,YUDIARTI T,ISROLI I,et al.Dietary supplementation of probiotics in poultry exposed to heat stress-a review[J].Annals of Animal Science,2017,17(3):591-604.  
[8] SONG Z H,CHENG K,ZHANG L L,et al.Dietary supplementation of enzymatically treated Artemisia annua could alleviate the intestinal inflammatory response in heat-stressed broilers[J].Journal of Thermal Biology,2017,69:184-190.
[9] WANG W C,YAN F F,HU J Y,et al.Supplementation of Bacillus subtilis-based probiotic reduces heat stress-related behaviors and inflammatory response in broiler chickens[J].Journal of Animal Science,2018,96(5):1654-1666.  
[10] FASELEH JAHROMI M,WESAM ALTAHER Y,SHOKRYAZDAN P,et al.Dietary supplementation of a mixture of Lactobacillus strains enhances performance of broiler chickens raised under heat stress conditions[J].International Journal of Biometeorology,2016,60(7):1099-1110.  
[11] ALUWONG T,SUMANU V O,AYO J O,et al.Daily rhythms of cloacal temperature in broiler chickens of different age groups administered with zinc gluconate and probiotic during the hot-dry season[J].Physiological Reports,2017,5(12):e13314.
[12] SONG J,XIAO K,KE Y L,et al.Effect of a probiotic mixture on intestinal microflora,morphology,and barrier integrity of broilers subjected to heat stress[J].Poultry Science,2014,93(3):581-589.  
[13] ATTIA Y A,AL-HARTHI M A,EL-SHAFEY A S,et al.Enhancing tolerance of broiler chickens to heat stress by supplementation with vitamin E,vitamin C and/or probiotics[J].Annals of Animal Science,2017,17(4):1155-1169.  
[14] OGBUAGU N E,ALUWONG T,AYO J O,et al.Effect of fisetin and probiotic supplementation on erythrocyte osmotic fragility,malondialdehyde concentration and superoxide dismutase activity in broiler chickens exposed to heat stress[J].Journal of Veterinary Medical Science,2018,80(12):1895-1900.  
[15] BERNARDEAU M,LEHTINEN M J,FORSSTEN S D,et al.Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality[J].Journal of Food Science and Technology,2017,54(8):2570-2584.  
[16] 夏涛,白华毅,王乙茹,等.饲用凝结芽孢杆菌在肉鸡生产中的应用研究进展[J].黑龙江畜牧兽医,2018(23):35-38.
[17] 王乙茹,柳成东,白华毅,等.一株凝结芽孢杆菌YNAU 5517对热应激肉鸡生长性能影响的初探[J].饲料工业,2019,40(5):16-22.
[18] AL-FATAFTAH A R,ABDELQADER A.Effects of dietary Bacillus subtilis on heat-stressed broilers performance,intestinal morphology and microflora composition[J].Animal Feed Science and Technology,2014,198:279-285.
[19] 杨梅梅.中药复方与益生素对热应激下肉鸡肠道菌群及生长的影响[D].硕士学位论文.广州:华南农业大学,2016.
[20] GHAREEB K,AWAD W A,ZEBELI Q,et al.Deoxynivalenol in chicken feed alters the vaccinal immune response and clinical biochemical serum parameters but not the intestinal and carcass characteristics[J].Journal of Animal Physiology and Animal Nutrition,2016,100(1):53-60.  
[21] LUO J X,SONG J,LIU L Z,et al.Effect of epigallocatechin gallate on growth performance and serum biochemical metabolites in heat-stressed broilers[J].Poultry Science,2018,97(2):599-606.  
[22] MOHAMMED A A,JIANG S,JACOBS J A,et al.Effect of a synbiotic supplement on cecal microbial ecology,antioxidant status,and immune response of broiler chickens reared under heat stress[J].Poultry Science,2019,98(10):4408-4415.  
[23] LARA L J,ROSTAGNO M H.Impact of heat stress on poultry production[J].Animals,2013,3(2):356-369.  
[24] SOHAIL M U,HUME M E,BYRD J A,et al.Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress[J].Poultry Science,2012,91(9):2235-2240.  
[25] KAMEL N N,AHMED A M H,MEHAISEN G M K,et al.Depression of leukocyte protein synthesis,immune function and growth performance induced by high environmental temperature in broiler chickens[J].International Journal of Biometeorology,2017,61(9):1637-1645.  
[26] SHI D Y,BAI L,QU Q,et al.Impact of gut microbiota structure in heat-stressed broilers[J].Poultry Science,2019,98(6):2405-2413.  
[27] HE S P,LI S,AROWOLO M A,et al.Effect of resveratrol on growth performance,rectal temperature and serum parameters of yellow-feather broilers under heat stress[J].Animal Science Journal,2019,90(3):401-411.  
[28] SOHAIL M U,IJAZ A,YOUSAF M S,et al.Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic:dynamics of cortisol,thyroid hormones,cholesterol,C-reactive protein,and humoral immunity[J].Poultry Science,2010,89(9):1934-1938.  
[29] HALDAR S,GHOSH T K,TOSHIWATI,et al.Effects of yeast (Saccharomyces cerevisiae) and yeast protein concentrate on production performance of broiler chickens exposed to heat stress and challenged with Salmonella enteritidis[J].Animal Feed Science and Technology,2011,168(1/2):61-71.
[30] CHEN X Y,WEI P P,XU S Y,et al.Rectal temperature as an indicator for heat tolerance in chickens[J].Animal Science Journal,2013,84(11):737-739.  
[31] VAN GOOR A,ASHWELL C M,PERSIA M E,et al.Unique genetic responses revealed in RNA-seq of the spleen of chickens stimulated with lipopolysaccharide and short-term heat[J].PLoS One,2017,12(2):e0171414.
[32] SELVAM R,SURESH S,SARAVANAKUMAR M,et al.Alleviation of heat stress by a polyherbal formulation,PhytoceeTM:impact on zootechnical parameters,cloacal temperature,and stress markers[J].Pharmacognosy Research,2018,10(1):1-8.
[33] AL-ZGHOUL M B,ALLIFTAWI A R S,SALEH K M M,et al.Expression of digestive enzyme and intestinal transporter genes during chronic heat stress in the thermally manipulated broiler chicken[J].Poultry Science,2019,98(9):4113-4122.  
[34] INOUE H,SHIMAMOTO S,TAKAHASHI H,et al.Effects of astaxanthin-rich dried cell powder from Paracoccus carotinifaciens on carotenoid composition and lipid peroxidation in skeletal muscle of broiler chickens under thermo-neutral or realistic high temperature conditions[J].Animal Science Journal,2019,90(2):229-236.  
[35] ZANGENEH S,TORKI M,LOTFOLLAHIAN H,et al.Effects of dietary supplemental lysophospholipids and vitamin C on performance,antioxidant enzymes,lipid peroxidation,thyroid hormones and serum metabolites of broiler chickens reared under thermoneutral and high ambient temperature[J].Journal of Animal Physiology and Animal Nutrition,2018,102(6):1521-1532.  
[36] EL-DEEP M H,DAWOOD M A O,ASSAR M H,et al.Dietary Moringa oleifera improves growth performance,oxidative status,and immune related gene expression in broilers under normal and high temperature conditions[J].Journal of Thermal Biology,2019,82:157-163.
[37] TANG S,YIN B,XU J,et al.Rosemary reduces heat stress by inducing CRYAB and HSP70 expression in broiler chickens[J].Oxidative Medicine and Cellular Longevity,2018,2018:7014126.
[38] NYBLOM H,BERGGREN U,BALLDIN J,Et al.High AST/ALT ratio may indicate advanced alcoholic liver disease rather than heavy drinking[J].Alcohol and Alcoholism,2004,39(4):336-339.  
[39] SAHIN N,HAYIRLI A,ORHAN C,et al.Effects of the supplemental chromium form on performance and oxidative stress in broilers exposed to heat stress[J].Poultry Science,2017,96(12):4317-4324.  
[40] AKBARIAN A,MICHIELS J,DEGROOTE J,et al.Association between heat stress and oxidative stress in poultry;mitochondrial dysfunction and dietary interventions with phytochemicals[J].Journal of Animal Science and Biotechnology,2016,7:37.
[41] 李秋粉.益生菌对热应激肉鸡肠道屏障功能的影响[D].硕士学位论文.南昌:江西农业大学,2017.
[42] ALHENAKY A,ABDELQADER A,ABUAJAMIEH M,et al.The effect of heat stress on intestinal integrity and Salmonella invasion in broiler birds[J].Journal of Thermal Biology,2017,70:9-14.
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