Effects of Yeast Hydrolysate on Growth Performance, Muscle Quality, Hepatopancreases Antioxidant Indexes and Tissue Morphology of Juvenile Black Carp (Mylopharyngodon piceus)

JIANG Minmin; YE Jinyun; SHAO Xianping; LIN Yifan; WU Chenglong; JIANG Lijun; CEN Xinnuo; ZHOU Qicun

doi:10.3969/j.issn.1006-267x.2020.05.042

2020 , Vol. 32 >Issue 5: 2326 - 2341

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.042

AQUATIC ANIMAL NUTRITION AND FEED

Effects of Yeast Hydrolysate on Growth Performance, Muscle Quality, Hepatopancreases Antioxidant Indexes and Tissue Morphology of Juvenile Black Carp (Mylopharyngodon piceus)

JIANG Minmin ,
YE Jinyun ,
SHAO Xianping ,
LIN Yifan ,
WU Chenglong ,
JIANG Lijun ,
CEN Xinnuo ,
ZHOU Qicun

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1. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, College of Life Sciences, Huzhou University, Huzhou 313000, China

Received date: 2019-11-13

Online published: 2020-05-15

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Abstract

This experiment was conducted to estimate the effects of dietary levels of yeast hydrolysate (YHY) on growth performance, whole body and muscle amino acid composition, hepatopancreases antioxidant indexes and tissue morphology of juvenile black carp (Mylopharyngodon piceus). The YHY of 0, 0.5%, 1.0%, 1.5%, 2.0% and 2.5% were added to a basic diet, and six isonitrogenous and isoenergetic diets were prepared[named YHY0 (control), YHY0.5, YHY1.0, YHY1.5, YHY2.0 and YHY2.5, respectively]. A total of 720 juvenile black carps with an initial average body weight of (5.53±0.14) g were randomly divided into six groups with four replicates per group and 30 black carps per replicate. The experiment was carried out in the circulating water system for 8 weeks. The results showed as follow:1) the specific growth rate (SGR) of juvenile black carp increased first and then decreased, and the protein efficiency (PER) increased and then gradually smooth. YHY1.5 and YHY2.0 groups had significantly higher SGR compared with the control group (P<0.05), and there was no significant difference compared with the YHY2.5 group (P>0.05); PER in the YHY1.5, YHY2.0 and YHY2.5 groups was significantly higher than that in the control group (P<0.05). 2) Dietary different levels of YHY had no significant influences on dry matter, crude protein and ash contents of whole body (P>0.05). The crude lipid of whole body showed an upward trend and reached the maximum in the YHY2.5 group, which was significantly higher than that in the control group (P<0.05). Dietary different levels of YHY had no significant influence on day matter, crude protein and crude lipid contents of juvenile black carp muscle (P>0.05). The ash contents in the YHY2.0 and YHY2.5 groups was significantly higher than that in the control group (P<0.05). 3) The essential amino acids and flavor amino acids contents of whole body and muscle in all supplemental groups were significantly higher than those in control group (P<0.05). At the same time, the amino acid score (AAS), chemical score (CS) and essential amino acid index (EAAI) were significantly improved with the increasing supplementation level of YHY (P<0.05). 4) The total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD) activity of juvenile black carp were increased firstly and then decreased. And the T-AOC and T-SOD activity in the 1.5% group reached the highest, which was significantly higher than that in the control group (P<0.05). There was no significant influence on catalase (CAT) activity in hepatopancreases of juvenile black carp (P>0.05). Dietary YHY different level could improve the lipid deposition of hepatopancreases. In conclusion, based on the data analysis of SGR and PER, dietary YHY supplemental levels of 1.57% to 2.02% can significantly improve the growth performance, and at the same time, the YHY can improve the whole body and muscle amino acid composition, the quality of muscle and the hepatopancreases antioxidant enzyme activity of juvenile black carp. YHY does not adversely affect the hepatopancreases tissue morphology of juvenile black carp.

Key words： yeast hydrolysate; Mylopharyngodon piceus; growth performance; amino acid; muscle quality; hepatopancreases antioxidant enzymes; tissue morphology

Cite this article

JIANG Minmin , YE Jinyun , SHAO Xianping , LIN Yifan , WU Chenglong , JIANG Lijun , CEN Xinnuo , ZHOU Qicun . Effects of Yeast Hydrolysate on Growth Performance, Muscle Quality, Hepatopancreases Antioxidant Indexes and Tissue Morphology of Juvenile Black Carp (Mylopharyngodon piceus)[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2326 -2341 . DOI: 10.3969/j.issn.1006-267x.2020.05.042

References

[1] 冷向军.青鱼的营养需求与配合饲料研究[J].饲料工业,2016,37(6):1-7.
[2] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2019中国渔业统计年鉴[M].北京:中国农业出版社,2019.
[3] 叶金云.青鱼营养与配合饲料研究现状与展望(一)[J].科学养鱼,2011(11):12-14.
[4] 柳茜,杨文娇,吴振,等.酵母水解物对大菱鲆幼鱼非特异性免疫及抗应激能力的影响[J].饲料工业,2015,36(18):33-37.
[5] FERREIRA I M P L V O,PINHO O,VIEIRA E,et al.Brewer's Saccharomyces yeast biomass:characteristics and potential applications[J].Trends in Food Science & Technology,2010,21(2):77-84.

[6] HOSEINIFAR S H,MIRVAGHEFI A,MERRIFIELD D L.The effects of dietary inactive brewer's yeast Saccharomyces cerevisiae var. ellipsoideus on the growth,physiological responses and gut microbiota of juvenile beluga (Huso huso)[J].Aquaculture,2011,318(1/2):90-94.
[7] ZHOU M,LIANG R S,MO J F,et al.Effects of brewer's yeast hydrolysate on the growth performance and the intestinal bacterial diversity of largemouth bass (Micropterus salmoides)[J].Aquaculture,2018,484:139-144.
[8] ORTUÑO J,CUESTA A,RODRIÍGUEZ A,et al.Oral administration of yeast,Saccharomyces cerevisiae,enhances the cellular innate immune response of gilthead seabream (Sparus aurata L.)[J].Veterinary Immunology and Immunopathology,2002,85(1/2):41-50.
[9] ANDREWS S R,SAHU N P,PAL A K,et al.Yeast extract,brewer's yeast and spirulina in diets for Labeo rohita fingerlings affect haemato-immunological responses and survival following Aeromonas hydrophila challenge[J].Research in Veterinary Science,2011,91(1):103-109.

[10] SHEIKHZADEH N,HEIDARIEH M,PASHAKI A K,et al.Hilyses,fermented Saccharomyces cerevisiae,enhances the growth performance and skin non-specific immune parameters in rainbow trout (Oncorhynchus mykiss)[J].Fish & Shellfish Immunology,2012,32(6):1083-1087.

[11] 陈建明,沈斌乾,潘茜,等.饲料蛋白和脂肪水平对青鱼大规格鱼种生长和体组成的影响[J].水生生物学报,2014,38(4):699-705.
[12] 王道尊,龚希章,刘玉芳.饲料中脂肪的含量对青鱼鱼种生长的影响[J].水产学报,1987,11(1):23-28.
[13] 蔡春芳,王永玲,陈立侨,等.饲料糖种类和水平对青鱼、鲫生长和体成分的影响[J].中国水产科学,2006(3):118-125.
[14] 明建华,叶金云,张易祥,等.青鱼对8种饲料原料中营养物质的表观消化率[J].动物营养学报,2012,24(10):2050-2058.
[15] 刘沛,叶金云,邵仙萍,等.饲料中小肽豆粕替代鱼粉对青鱼幼鱼生长及其体组成的影响[J].浙江海洋学院学报(自然科学版),2014,33(1):72-78.
[16] 陈炼,吴成龙,张易祥,等.维生素A对青鱼幼鱼生长、代谢、抗氧化能力和免疫能力的影响[J].动物营养学报,2018,30(7):2594-2605.
[17] 黄云,胡毅,文华,等.维生素E对青鱼幼鱼生长、免疫及抗氨氮胁迫能力的影响[J].水生生物学报,2013,37(3):507-514.
[18] FAO/WHO.Energy and protein requirements[M].Rome:FAO Nutrition Meeting Report Series,1973:52.
[19] 蔡完其(译).养鱼饲料学[M].北京:中国农业出版社,1980:114-115.
[20] 施永海,张根玉,刘永士,等.野生及养殖哈氏对虾肌肉营养成分的分析比较[J].水产学报,2013,37(5):768-776.
[21] 曾本和,杨文娇,吴振,等.酵母水解物对加州鲈幼鱼生长性能及免疫酶指标的影响[J].饲料工业,2016,37(14):11-15.
[22] HASSAAN M S,MAHMOUD S A,JARMOLOWICZ S,et al.Effects of dietary baker's yeast extract on the growth,blood indices and histology of Nile tilapia (Oreochromis niloticus L.) fingerlings[J].Aquaculture Nutrition,2018,24(6):1709-1717.

[23] YUAN X Y,LIU W B,LIANG C,et al.Effects of partial replacement of fish meal by yeast hydrolysate on complement system and stress resistance in juvenile Jian carp (Cyprinus carpio var. Jian)[J].Fish & Shellfish Immunology,2017,67:312-321.
[24] 曾本和,向枭,杨文娇,等.酵母水解物对草鱼生长性能和体成分的影响[J].水产养殖,2015,36(16):16-19.
[25] JARMOŁOWICZ S,ZAKEŚ Z,SIWICKI A,et al.Effects of brewer's yeast extract on growth performance and health of juvenile pikeperch Sander lucioperca (L.)[J].Aquaculture Nutrition,2012,18(4):457-464.

[26] 时博,郁欢欢,梁晓芳,等.酵母水解物对大口黑鲈生长性能、血浆生化指标以及肝胰脏组织健康的影响[J].动物营养学报,2018,30(7):2772-2781.
[27] 张德瑞.乳制品-酵母益生元(Grobiotic-A)对星斑川鲽(Platichthys stellatus)幼鱼生长、消化及非特异性免疫影响的研究[D].硕士学位论文.上海:上海海洋大学,2013.
[28] 邱燕.三种微生态制剂对草鱼(Ctenopharyngodon idellus)生长性能、生理机能及肠道黏膜的影响[D].硕士学位论文.苏州:苏州大学,2010.
[29] ABDEL-TAWWAB M,ABDEL-RAHMAN A M,ISMAEL N E M.Evaluation of commercial live bakers' yeast,Saccharomyces cerevisiae as a growth and immunity promoter for Fry Nile tilapia,Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila[J].Aquaculture,2008,280(1/2/3/4):185-189.
[30] OZÓRIO R O A,TURINI B G S,MÔRO G V,et al.Growth,nitrogen gain and indispensable amino acid retention of pacu (Piaractus mesopotamicus,Holmberg 1887) fed different brewers yeast (Saccharomyces cerevisiae) levels[J].Aquaculture Nutrition,2010,16(3):276-283.
[31] LUNGER A N,CRAIG S R,MCLEAN E.Replacement of fish meal in cobia (Rachycentron canadum) diets using an organically certified protein[J].Aquaculture,2006,257(1/2/3/4):393-399.
[32] 张莉莉,梁萌青,徐后国,等.饲料中添加磷虾水解物对大菱鲆幼鱼生长性能、体组成及相关酶活性的影响[J].水生生物学报,2017,41(3):497-505.
[33] 陈春燕,姚杰宝,黄旭雄,等.饲料中鱼粉用量及添加酵母提取物对中华绒螯蟹幼蟹蜕壳及蟹体游离氨基酸组成的影响[J].中国水产,2013(11):58-61.
[34] 王武刚.酵母提取物替代鱼粉在凡纳滨对虾饲料中的应用研究[D].硕士学位论文.上海:上海海洋大学,2012.
[35] CARRIGAN C N,IMPERIALI B.The engineering of membrane-permeable peptides[J].Analytical Biochemistry,2005,341(2):290-298.

[36] WEBB K E,Jr.Intestinal absorption of protein hydrolysis products:a review[J].Journal of Animal Science,1990,68(9):3011-3022.

[37] 蒋步国,冯健,黄钧,等.酪蛋白小肽和氨基酸对草鱼血液循环和组织蛋白质合成的影响[J].海洋与湖沼,2010,41(1):75-79.
[38] BAMBA T,KUSE K,OBATA H,et al.Effects of small peptides as intraluminal substrates on transport carriers for amino acids and peptides[J].Journal of Clinical Biochemistry and Nutrition,1993,15:33-42.
[39] 王际英,姜柯君,夏斌,等.小肽对星斑川鲽幼鱼消化酶活性、抗氧化能力和生化组成的影响[J].中国水产科学2014,21(6):1154-1164.
[40] 李清.小肽对鲤鱼生产性能、肉质及血液理化指标的影响[D].硕士学位论文.长沙:湖南农业大学,2004.
[41] REYES-BECERRIL M,TOVAR-RAMÍREZ D,ASCENCIO-VALLE F,et al.Effects of dietary live yeast Debaryomyces hansenii on the immune and antioxidant system in juvenile leopard grouper Mycteroperca rosacea exposed to stress[J].Aquaculture,2008,280(1/2/3/4):39-44.
[42] YANG S P,WU Z H,JIAN J C,et al.Effect of marine red yeast Rhodosporidium paludigenum on growth and antioxidant competence of Litopenaeus vannamei[J].Aquaculture,2010,309(1/2/3/4):62-65.
[43] 苗新,曹娟娟,徐玮,等.核苷酸对大黄鱼生长性能、肠道形态和抗氧化能力的影响[J].水产学报,2014,38(8):1140-1148.
[44] ZHU F M,DU B,XU B J.A critical review on production and industrial applications of beta-glucans[J].Food Hydrocolloids,2016,52:275-288.
[45] DALMO R A,BØGWALD J. β-glucans as condutors of immune symphonies[J].Fish & Shellfish Immunology,2008,25:384-396.
[46] 张辽.β-葡聚糖对齐口裂腹鱼生长及非特异性免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2011.
[47] DAS B K,DEBNATH C,PATNAIK P,et al.Effect of β-glucan on immunity and survival of early stage of Anabas testudineus (Bloch)[J].Fish & Shellfish Immunology,2009,27(6):678-683.

[48] WONGSASAK U,CHAIJAMRUS S,KUMKHONG S,et al.Effects of dietary supplementation with β-glucan and synbiotics on immune gene expression and immune parameters under ammonia stress in Pacific white shrimp[J].Aquaculture,2015,436:179-187.
[49] 雷光高,叶继丹,宋奔奔,等.小肽对牙鲆幼鱼的生长、消化酶活性及肝胰脏抗氧化能力的影响[J].水产养殖,2008,29(3):1-4.
[50] 林启存,方长富,钟国防,等.小肽对凡纳滨对虾幼体生长性能及非特异性免疫能力的影响[J].浙江农业学报,2010,22(5):590-595.
[51] 杨凡,易建华,聂琴,等.酵母水解物在水产饲料中的应用[J].中国饲料,2016(16):41-43.
[52] 迟淑艳,聂琴,黄吴文,等.凡纳滨对虾饲料中酵母水解物替代鱼粉适宜比例的研究[J].水生生物学报,2016,40(4):728-735.
[53] 李洋.甘露寡糖对幼建鲤生长性能和非特异性免疫的影响[D].硕士学位论文.雅安:四川农业大学,2011.
[54] TORRECILLAS S,MAKOL A,CABALLERO M J,et al.Immune stimulation and improved infection resistance in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides[J].Fish & Shellfish Immunology,2007,23(5):969-981.

[55] 李潇,王玲,张春晓,等.啤酒酵母粉替代部分鱼粉对牛蛙生长、抗氧化能力以及肝胰脏和肠道组织形态学的影响[J].动物营养学报,2019,31(4):1864-1876.
[56] PANAGIOTIDOU M,NENGAS I,HENRY M,et al.Effect of different dietary levels of yeast extract (Nupro^®) on growth,feed utilisation and immune system of sea bass (Dicentrarchus labrax)[C]//Proceedings of the 9th Symposium on Oceanography & Fisheries,2009-Proceedings,Volume Ⅱ.[S.l.]:[s.n.],2009:1080-1084.
[57] 刘立鹤,张恒,聂伟,等.草鱼配合饲料添加酵母培养物对草鱼生长性能、肝肠功能的影响[J].武汉轻工大学学报,2014,33(1):34-39.

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