Effects of Feeding Acidified Milk on Fecal Microbial Diversity of Calves

GAO Yan; YIN Shuxin; WANG Lu; ZHANG Shuai; XI Juncheng; LIU Yuqin; QU Yongli

doi:10.3969/j.issn.1006-267x.2020.05.052

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 5: 2427 - 2439

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.05.052

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Effects of Feeding Acidified Milk on Fecal Microbial Diversity of Calves

GAO Yan ,
YIN Shuxin ,
WANG Lu ,
ZHANG Shuai ,
XI Juncheng ,
LIU Yuqin ,
QU Yongli

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College of Animal Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2019-10-31

Online published: 2020-05-15

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Abstract

This experiment was conducted to study the effects of feeding acidified milk on fecal microbial diversity of calves. A completely randomized trial design was used in this experiment. Ten healthy Holstein newborn female calves with similar height and body weight were selected from a large-scale dairy farm. They were randomly divided into 2 treatments with 5 calves in each treatment, and were fed pasteurized milk and acidified milk, respectively. The experiment lasted for 180 days, including 60 days of lactation. Fecal score was recorded daily and diarrhea rate was calculated. In addition, about 20 g of fecal samples were taken from the end of calf's rectum for 16S rDNA amplification and sequencing at days 50 and 90, and the samples were divided into 4 groups according to treatment and sampling time, which were PB group (samples form pasteurized milk treatment at day 50), PP group (samples form pasteurized milk treatment at day 90), AB group (samples form acidified milk treatment at day 50) and AP group (samples form acidified milk treatment at day 90). The results showed as follows:1) at different age stages during lactation period, the fecal score and the diarrhea rate of calves fed acidified milk were significantly lower than those fed pasteurized milk (P<0.05 or P<0.01). 2) A total of 1 227 017 Tags could be used for subsequent analysis. Operational taxonomic unit (OUT) cluster analysis by each group showed that the number of specific OTU in the feces of calves fed acidified milk were lower than those fed pasteurized milk. 3) The highest relative abundance of bacteria in samples of 4 groups was Firmicutes, followed by Bacteroidetes and Fusobacteria. 4) The LEfSe analysis result showed that species with significant differences in the AB group compared with other groups were Bacteroidaceae, Bacteroides, Faecalibacterium, Ruminiclostridium_9, Alloprevotella, Lachnospiraceae_UCG-004, Lachnospiraceae_UCG-010, Bacill, Lactobacillales, Blautia, and the most influential species were Bacteroidaceae and Bacteroides. In summary, feeding acidified milk can reduce the diarrhea rate, improve the fecal score, and can also regulate the balance of intestinal flora of calves to a certain degree.

Key words： calves; acidified milk; fecal microbial diversity

Cite this article

GAO Yan , YIN Shuxin , WANG Lu , ZHANG Shuai , XI Juncheng , LIU Yuqin , QU Yongli . Effects of Feeding Acidified Milk on Fecal Microbial Diversity of Calves[J]. Chinese Journal of Animal Nutrition, 2020 , 32(5) : 2427 -2439 . DOI: 10.3969/j.issn.1006-267x.2020.05.052

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