Effects of Linseed Oil on Long-Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs

XING Yueteng; YU Yuannian; XIE Chunyan; WU Xin; XIAO Dingfu; YANG Zhiwu; ZHANG Bin

doi:10.3969/j.issn.1006-267x.2020.06.016

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 6: 2567 - 2574

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.016

Effects of Linseed Oil on Long-Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs

XING Yueteng ,
YU Yuannian ,
XIE Chunyan ,
WU Xin ,
XIAO Dingfu ,
YANG Zhiwu ,
ZHANG Bin

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Resources and Environment, Hunan Agricultural University, Changsha 410128, China;
3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
4. Ningxiang Animal Husbandry and Veterinary Bureau, Changsha 410600, China

Received date: 2019-12-15

Online published: 2020-06-16

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Abstract

This experiment was conducted to study the linseed oil (LO) on fatty acid profile in longissimus dorsi muscle (LDM) and three different adipose tissues of Ningxiang pigs, and then investigate the underlying lipogenesis mechanisms. Thirty castrated female pigs with a similar age and average body weight of (43.21±0.64) kg were randomly allocated into 2 groups with 5 repetitions per group and 3 pigs per repetition. Pigs in control group were fed a basal diet, and those in experimental group were fed the basal diet supplemented with 2% LO for 56 days. The results showed that dietary supplemented with 2% LO significantly increased the contents of eicosenoic acid (C20:1), γ-linolenic acid (C18:3n-6) and α-linolenic acid (C18:3n-3) in LDM (P<0.05), and significantly decreased the content of arachidonic acid (C20:4n-6) in LDM compared with control group (P<0.05). The fatty acid contents in 3 adipose tissues influenced by 2% LO were analogous to those found in the LDM. The contents of linoleic acid (C18:2n-6c), C18:3n-6, C18:3n-3 and epoxyeicosatrienoic acid (C20:3n-6) in dorsal subcutaneous adipose (DSA), abdominal subcutaneous adipose (ASA) and perirenal adipose (PA) were significantly increased (P<0.05) accompanied by a corresponding decrease in oleic acid (C18:1n-9c) (P<0.05), C20:4n-6 (0.05≤P<0.10) in DSA and APA, as well as stearic acid (C18:0) in PA (P<0.05). Meanwhile, dietary supplemented with 2% LO could significantly the ratio of n-6/n-3 polyunsaturated fatty acids (PUFA) in 3 adipose tissues (P<0.05), and make the average n-6/n-3 PUFA ratio reduce to 5.92. Furthermore, dietary supplemented with 2% LO could down-regulate the expression of acetyl coenzyme A carboxylase α (ACCα), peroxisome proliferators-activated receptor γ (PPARγ), fatty acid desaturase 2 (FADS2) genes in DSA (P<0.05), and had an inhibitory tendency on the expression of peroxisome proliferators-activated receptor α (PPARα) gene in DSA (0.05≤P<0.10). These results indicate that dietary supplemented with 2% LO can significantly increase the content of C18:3n-3 in LDM, DSA, ASA and PA of Ningxiang pigs, and make the average n-6/n-3 PUFA ratio in three adipose tissues down to 5.92, which in the recommended range (4 to 6) of Dietary Guidelines for Chinese Residents (2016). The regulation of LO on lipid metabolism may be achieved through regulating the de novo synthesis of fatty acids and cell differentiation.

Key words： linseed oil; long-chain fatty acid; lipid metabolism; Ningxiang pigs

Cite this article

XING Yueteng , YU Yuannian , XIE Chunyan , WU Xin , XIAO Dingfu , YANG Zhiwu , ZHANG Bin . Effects of Linseed Oil on Long-Chain Fatty Acid Profile in Different Tissues of Ningxiang Pigs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2567 -2574 . DOI: 10.3969/j.issn.1006-267x.2020.06.016

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