Effects of Guanidinoacetic Acid Supplementation in No Animal Protein Diet on Growth Performance and Intestinal Barrier of Weaned Piglets

SITU Jinshui; WANG Jie; GAO Ping; WANG Zhonggang; ZHU Fang; YANG Fuling; JIANG Qingyan; ZHU Xiaotong; LUO Xufang

doi:10.3969/j.issn.1006-267x.2020.06.019

2020 , Vol. 32 >Issue 6: 2601 - 2610

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.019

Effects of Guanidinoacetic Acid Supplementation in No Animal Protein Diet on Growth Performance and Intestinal Barrier of Weaned Piglets

SITU Jinshui ,
WANG Jie ,
GAO Ping ,
WANG Zhonggang ,
ZHU Fang ,
YANG Fuling ,
JIANG Qingyan ,
ZHU Xiaotong ,
LUO Xufang

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1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Huahong Feed Technology Co., Ltd., Zhaoqing 526060, China;
3. Beijing Jundetongchuang Agriculture and Animal Husbandry Technology Co., Ltd., Beijing 100089, China

Received date: 2019-12-24

Online published: 2020-06-16

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Abstract

The objective of this study was to explore the effects of substitution of guanidinoacetic acid and soybean meal for fish meal on growth performance, serum physiological and biochemical indicators and intestinal barrier function of weaned piglets. A total of 204 Duroc×Landrace×Yorkshire crossbred weaned piglets aged at 42 days were randomly divided into 3 groups (4 replicates in each group and 17 pigs in each replicate) as the control group (fed a basal diet containing 3% fish meal from Peru), no animal protein group (3% fish meal in the control diet replaced by 3% dehulled soybean meal), and guanidinoacetic acid group (600 mg/kg guanidinoacetic acid supplementation in no animal protein diet). Nutrient levels and restricted amino acid contents were the same in all groups after dietary adjustment. The experiment lasted for 28 days. The results showed as follows: 1) compared with the control group, the 600 mg/kg guanidinoacetic acid supplementation in no animal protein diet significantly increased the final weight of weaned piglets (P<0.05), extremely significantly increased the average daily weight gain of weaned piglets (P<0.01), significantly reduced the feed to gain ratio (P<0.05), and there was no significant difference in the death rate of weaned piglets (P≥0.05). But the death rate of weaned piglets increased by 33.33% when 3% fish meal in the control diet replaced by 3% dehulled soybean meal, and while the death rate of weaned piglets decreased to the same level as that of the control group after adding appropriate amount of guanidinoacetic acid. 2) Compared with the control group, guanidinoacetic acid supplementation in no animal protein diet significantly increased the serum contents of total protein and globulin of weaned piglets (P<0.05), and had no significant effects on serum indices of lipid metabolism, glucose metabolism and related hormone (P≥0.05). 3) Compared with the control group, guanidinoacetic acid supplementation in no animal protein diet significantly reduced the villus height in jejunum of weaned piglets (P<0.05), but had no significant effects on the crypt depth, the ratio of villus height to crypt depth (V/C) in jejunum, and the villi height, crypt depth and V/C in duodenum and ileum (P≥0.05). 4) Compared with the control group, guanidinoacetic acid supplementation in no animal protein diet extremely significantly increased the occludin expression in duodenum and ileum of weaned piglets (P<0.01), significantly increased the claudin-1 expression in duodenum (P<0.05), significantly decreased the serum endotoxin content (P<0.05), extremely significantly increased the number of goblet cells in jejunum and ileum (P<0.01), and significantly increased the mRNA expression level of the anti-inflammatory factor interleukin-10 (IL-10) in ileum (P<0.05). Compared with the no animal protein group, guanidinoacetic acid supplementation in no animal protein diet extremely significantly increased the occludin expression in ileum (P<0.01), significantly increased the occludin expression in duodenum and claudin-1 expression in jejunum (P<0.05), and significantly decreased the serum endotoxin content (P<0.05). In conclusion, 600 mg/kg guanidinoacetic acid supplementation in no animal protein diet can effectively improve the intestinal barrier function of weaned piglets and promote the growth of piglets.

Key words： guanidineacetic acid; weaned piglets; growth performance; intestinal barrier

Cite this article

SITU Jinshui , WANG Jie , GAO Ping , WANG Zhonggang , ZHU Fang , YANG Fuling , JIANG Qingyan , ZHU Xiaotong , LUO Xufang . Effects of Guanidinoacetic Acid Supplementation in No Animal Protein Diet on Growth Performance and Intestinal Barrier of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2601 -2610 . DOI: 10.3969/j.issn.1006-267x.2020.06.019

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