Effects of Dietary L-Glutamine Supplementation on Plasma Biochemical Parameters, Immune Performance, Intestinal Inflammatory Factors Expression and Mucosal Immune of Broilers Challenged by Lipopolysaccharide

ZHANG Bolin; YANG Qian; LIU Ning; SONG Peiyong; XIE Yuxiao; WANG Qingrong; SUN Zewei

doi:10.3969/j.issn.1006-267x.2020.06.020

2020 , Vol. 32 >Issue 6: 2611 - 2623

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.020

Effects of Dietary L-Glutamine Supplementation on Plasma Biochemical Parameters, Immune Performance, Intestinal Inflammatory Factors Expression and Mucosal Immune of Broilers Challenged by Lipopolysaccharide

ZHANG Bolin ,
YANG Qian ,
LIU Ning ,
SONG Peiyong ,
XIE Yuxiao ,
WANG Qingrong ,
SUN Zewei

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1. Key Laboratory of Conservation and Utilization of Characteristic Animal Resources in Chishui River Basin, Department of Biology and Agriculture, Zunyi Normal College, Zunyi 523006, China;
2. Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received date: 2020-01-31

Online published: 2020-06-16

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Abstract

The present study aimed to investigate the effects of dietary L-glutamine (L-Gln)supplementation on plasma biochemical parameters, immune performance, intestinal inflammatory factors expression and mucosal immune of broilers by lipopolysaccharide (LPS) challenge. A total of 120 one-day-old Arbor Acres (AA) broilers with good health and similar initial body weight were arranged in a 2×2 factorial design to 4 groups (groups A, B, C and D) with 5 replicates per group and 6 broilers per replicate. Broilers in groups A and C were fed the control diets (basal diet supplemented with 1.22% alanine), and broilers in groups B and D were fed the L-Gln diets (basal diet supplemented with 1% L-Gln). At day 16 and day 21, broilers in groups B and D were administered with LPS (500 μg/kg BW) by intraperitoneal injection, and broilers in groups B and D were administered with equivalent saline by intraperitoneal injection. The diet rather than water was cut off before 12 h of injection. The experiment lasted for 21 days. The results were as follows: 1) LPS challenge significantly increased the total protein (TP), uric acid (UA) contents and alanine aminotransferase (ALT), aspartate transaminase (AST) activities in plasma of broilers (P<0.05), significantly decreased the thymus index, spleen index and bursa of Fabricius index (P<0.05), significantly decreased the relative length of duodenum, relative weight of duodenum, relative length of jejunum, relative weight of jejunum, relative length of ileum and relative weight of ileum (P<0.05), significantly increased the mRNA expression levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in duodenum, jejunum and ileum (P<0.05), significantly increased the content of secretory immunoglobulin A (sIgA) in jejunal mucosa (P<0.05), and significantly increased the mRNA expression levels of mucin 1 (MUC1) and mucin 2 (MUC2) in jejunal mucosa (P<0.05). 2) Dietary L-Gln supplementation significantly decreased the TP, UA contents and ALT, AST activities in plasma of broilers (P<0.05), significantly increased the thymus index, spleen index and bursa of Fabricius index (P<0.05), significantly increased the relative length of duodenum, relative weight of duodenum, relative length of jejunum, relative weight of jejunum, relative length of ileum and relative weight of ileum (P<0.05), significantly decreased the mRNA expression levels of TNF-α and IL-6 in duodenum (P<0.05), significantly decreased the mRNA expression levels of TNF-α and IL-1β in jejunum and ileum (P<0.05), significantly decreased the content of sIgA in jejunal mucosa (P<0.05), and significantly decreased the mRNA expression levels of MUC1 and MUC2 in jejunal mucosa (P<0.05). In conclusion, dietary 1% L-Gln supplementation can improve the immune performance of broilers, enhance the intestinal mucosal immune function, and can partly effectively alleviate intestinal inflammatory responses of broilers induced by LPS.

Key words： L-glutamine; LPS challenge; immune performance; inflammatory response; intestinal mucosal immune

Cite this article

ZHANG Bolin , YANG Qian , LIU Ning , SONG Peiyong , XIE Yuxiao , WANG Qingrong , SUN Zewei . Effects of Dietary L-Glutamine Supplementation on Plasma Biochemical Parameters, Immune Performance, Intestinal Inflammatory Factors Expression and Mucosal Immune of Broilers Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2611 -2623 . DOI: 10.3969/j.issn.1006-267x.2020.06.020

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