Effects of Different Combinations of Cysteamine and Rumen-Protected Methionine on Lactation Performance, Rumen Microbial Protein Production and Nitrogen Excretion of Dairy Cows

ZHANG Zikun; ZHANG Yuli; GAO Xulei; SUN Guoqiang

doi:10.3969/j.issn.1006-267x.2020.06.028

2020 , Vol. 32 >Issue 6: 2690 - 2699

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.028

Effects of Different Combinations of Cysteamine and Rumen-Protected Methionine on Lactation Performance, Rumen Microbial Protein Production and Nitrogen Excretion of Dairy Cows

ZHANG Zikun ,
ZHANG Yuli ,
GAO Xulei ,
SUN Guoqiang

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1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109 China;
2. Jinan Animal Husbandry Technology Extension Station, Jinan 250306, China;
3. Agricultural and Rural Bureau of Laoshan, Qingdao 266061, China

Received date: 2019-12-02

Online published: 2020-06-16

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Abstract

This experiment was conducted to determine the effects of different combinations of cysteamine (CS) and rumen-protected methionine (RPMet) on lactation performance, rumen microbial protein (MCP) production and nitrogen excretion of dairy cows. Forty Holstein lactating cows with similar age, body weight, parity, milk yield, milk composition and lactation days were selected and randomly divided into 10 groups with 4 cows in each group. Cows in the control group (C) was fed a basic diet, and cows in experimental groups was supplemented with different combinations of CS and RPMet. The supplemental levels of CS were 13 (L), 15 (M) and 17 (H) g/ (d·head), respectively; and the supplemental levels of RPMet were 23 (L), 25 (M), 27 (H) g/ (d·head), respectively. A total of 9 experimental groups were designed, which were named as LL, LM, LH, ML, MM, MH, HL, HM and HH groups (first letter indicated CS supplemental level, and the second letter indicated RPMet supplemental level), respectively. The pre-experiment lasted for 15 days, and the experiment lasted for 60 days. The results showed as follows: 1) in terms of the milk yield, all experimental groups were significantly higher than the C group (P<0.05 or P<0.01), the HL group had the largest increase range, which was 19.76% higher than the C group. 2) In terms of the milk composition, the milk fat rate of LL, LH, ML, MM, MH, HL, HM and HH groups was significantly higher than that of the C group (P<0.01), the milk protein rate of LH, HL and HH groups was significantly higher than that of the C group (P<0.01), and the milk somatic cell count of ML and HH groups was significantly lower than that of the C group (P<0.01). 3) In terms of the rumen MCP production, the HL group was significantly higher than the C group (P<0.01). 4) In terms of the total nitrogen excretion, all experimental groups were significantly lower than the C group (P<0.01); in terms of the apparent digestibility of nitrogen, all experimental groups were significantly higher than the C group (P<0.01). In combination with various test indicators, the 17 g/(d·head) CS +23 g/(d·head) RPMet is the best combination.

Key words： cysteamine; rumen-protected methionine; lactation performance; rumen microbial protein; nitrogen excretion

Cite this article

ZHANG Zikun , ZHANG Yuli , GAO Xulei , SUN Guoqiang . Effects of Different Combinations of Cysteamine and Rumen-Protected Methionine on Lactation Performance, Rumen Microbial Protein Production and Nitrogen Excretion of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2690 -2699 . DOI: 10.3969/j.issn.1006-267x.2020.06.028

References

[1] 刘威,李发弟,赵圃毅,等.半胱胺盐酸盐对泌乳奶牛血液抗氧化能力的影响[J].甘肃农业大学学报,2015,50(3):16-22.
[2] 沈赞明,张荣飞,解红梅,等.半胱胺盐酸盐对泌乳20-42周奶牛产奶性能和部分免疫指标的影响[J].畜牧兽医学报,2005,36(7):667-673.
[3] 沈赞明,张荣飞,陈峰,等.复合半胱胺盐酸盐对泌乳后期奶牛产奶性能的影响[J].中国畜牧兽医,2004,31(8):9-10.
[4] 王玲,孙友德,刘锡武,等.半胱胺对奶牛瘤胃微生物蛋白产量、产奶性能和氮排泄的影响[J].动物营养学报,2015,27(4):1262-1269.
[5] 孙博非,余超,曹阳春,等.过瘤胃蛋氨酸对围产期奶牛代谢及健康的调控作用及机理[J].动物营养学报,2018,30(3):829-836.
[6] ZHOU Z,BULGARI O,VAILATI-RIBONI M,et al.Rumen-protected methionine compared with rumen-protected choline improves immunometabolic status in dairy cows during the peripartal period[J].Journal of Dairy Science,2016,99(11):8956-8969.

[7] 张成喜,孙友德,刘锡武,等.过瘤胃蛋氨酸对奶牛瘤胃微生物蛋白产量、产奶性能和氮排泄的影响[J].动物营养学报,2017,29(5):1759-1766.
[8] 任相全,毛一鹏.山东配合饲料资源应用商务章典[M].济南:山东人民出版社,2012.
[9] 朱雯.粗料来源对奶牛乳蛋白前体物生成与生产性能的影响与机制研究[D].博士学位论文.杭州:浙江大学,2013.
[10] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 6435-2006饲料中水分和其他挥发性物质含量的测定[S].北京:中国标准出版社,2007.
[11] 国家市场监督管理总局,国家标准化管理委员会.GB/T 6432-2018饲料中粗蛋白的测定凯氏定氮法[S].北京:中国标准出版社,2018.
[12] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 20806-2006饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社,2007.
[13] 中华人民共和国国家质量监督检验检疫总局.GB/T 6436-2002饲料中钙的测定[S].北京:中国标准出版社,2002.
[14] 中华人民共和国国家质量监督检验检疫总局.GB/T 6437-2002饲料中总磷的测定分光光度法[S].北京:中国标准出版社,2002.
[15] 中华人民共和国农业部.NY/T 1459-2007饲料中酸性洗涤纤维的测定[S].北京:农业出版社,2008.
[16] CHEN X B,MEJIA A T,KYLE D J,et al.Evaluation of the use of the purine derivative:creatinine ratio in spot urine and plasma samples as an index of microbial protein supply in ruminants:studies in sheep[J].The Journal of Agricultural Science,1995,125(1):137-143.

[17] 王虎成,龙瑞军,马亚玲,等.尿嘌呤衍生物估测瘤胃微生物蛋白产量的原理及研究进展[J].饲料工业,2008,29(1):47-51.
[18] 夏伦志,蔡东,熊国远,等.半胱胺补饲模式对奶牛生产性能及血清胰岛素水平的影响[J].中国畜牧杂志,2005,41(2):27-29.
[19] 芦娜,潘振亮,陈龙宾,等.日粮添加过瘤胃氨基酸对高产奶牛生产性能、乳成分和经济效益的影响[J].饲料工业,2019,40(5):44-47.
[20] 黄红英,贺建华.半胱胺在养猪生产中的应用[J].中国饲料,2007(17):18-21.
[21] 郭冬生,司国利.半胱胺的生物学功能与作用机制[J].养殖与饲料,2010(8):63-69.
[22] 肖玉梅,唐明红.日粮中添加吡啶羧酸铬与半胱胺复合剂对肥育猪生产性能和胴体品质的影响[J].饲料工业,2011,32(11):21-24.
[23] 甘佳,付茂忠,王巍,等.日粮中添加过瘤胃脂肪和过瘤胃蛋氨酸对荷斯坦奶牛生产性能的影响[J].四川畜牧兽医,2017,44(11):32-34.
[24] XU S,HARRISON J H,CHALUPA W,et al.The effect of ruminal bypass lysine and methionine on milk yield and composition of lactating cows[J].Journal of Dairy Science,1998,81(4):1062-1077.

[25] ZHANG Y N,BALA V,MAO Z H,et al.A concise review of quantification methods for determination of vitamin K in various biological matrices[J].Journal of Pharmaceutical and Biomedical Analysis,2019,169:133-141.
[26] 韩新燕,汪以真.半胱胺对动物的作用及应用效果[J].饲料工业,2000,21(12):29-30.
[27] 毕晓华,张晓明.过瘤胃保护蛋氨酸对奶牛氨基酸代谢和血液生化指标的影响[J].饲料研究,2014(21):48-53.
[28] 李海霞,杨美英,吴文海,等.过瘤胃蛋氨酸对黔北麻羊生长性能、养分表观消化率、血浆生化指标及瘤胃发酵的影响[J].动物营养学报,2019,31(6):2933-2940.
[29] 沈赞明,解红梅.半胱胺盐酸盐和蛋氨酸赖氨酸对山羊细胞免疫的影响[J].中国兽医科技,2004,34(11):29-34.
[30] 朱佳丽,李元晓,陈欣,等.过瘤胃蛋氨酸对奶牛免疫功能的影响[J].饲料研究,2018(5):58-61.
[31] 刘贵莲,王之盛,周安国.新型饲料添加剂——半胱胺[J].饲料工业,2006,27(22):4-8.
[32] 刘宗柱,沈延法,韩正康.半胱胺对绵羊消化代谢的影响[J].畜牧兽医学报,1996,27(3):212-219.
[33] 任延利.半胱胺在养猪生产中的应用[J].养殖与饲料,2014(10):32-35.
[34] 韦学玉,阎宏,魏智清.新型饲料添加剂——半胱胺的研究进展[J].畜牧与饲料科学,2006,27(5):69-71.
[35] 密士恒,王晓凤,马慧.瘤胃保护性蛋氨酸对肉羊氮代谢及生长性能的影响[J].中国畜牧杂志,2010,46(7):43-45.
[36] 张成喜,滕乐帮,刘晓,等.过瘤胃蛋氨酸和肉桂醛对奶牛瘤胃微生物蛋白产量和养分消化率的影响[J].中国畜牧杂志,2017,53(9):92-96.
[37] CHACHER B,ZHU W,YE J A,et al.Effect of dietary N-carbamoylglutamate on milk production and nitrogen utilization in high-yielding dairy cows[J].Journal of Dairy Science,2014,97(4):2338-2345.

[38] 刘月琴,张英杰,杨庆华,等.不同剂量半胱胺对绵羊消化代谢和生长发育的影响[J].黑龙江畜牧兽医,2006(10):50-51.
[39] 王忠艳.半胱胺在调节动物生长中的应用[J].中国畜牧兽医,2005,32(5):8-11.
[40] 陈丛亮,杨磊,杨洪勋,等.半胱胺盐酸盐对家禽营养物质消化、吸收和利用率的研究[J].饲料研究,2009(5):19-21.
[41] ABBASI I H R,ABBASI F,LIU L H,et al.Rumen-protected methionine a feed supplement to low dietary protein:effects on microbial population,gases production and fermentation characteristics[J].AMB Express,2019,9(1):93.
[42] 杨志强.日粮氨基酸配比对奶牛产奶性能和氮利用的影响[D].硕士学位论文.杭州:浙江大学,2009.
[43] SINCLAIR K D,GARNSWORTHY P C,MANN G E,et al.Reducing dietary protein in dairy cow diets:implications for nitrogen utilization,milk production,welfare and fertility[J].Animal,2014,8(2):262-274.

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