Effects of Different Levels of Tannin in Diets on Rumen Bacteria, Methanogens Number and Rumen Archaea Diversity in Sheep

  • LI Li ,
  • LI Dabiao ,
  • WANG Jingyao ,
  • XIE Yongfang ,
  • LI Zinan
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  • Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2019-12-31

  Online published: 2020-06-16

Abstract

This experiment was aimed to investigate the effects of different levels of tannin in diets on ruminal bacteria and methanogens number, as well as ruminal archaea diversity in sheep. A total of 15 healthy sheep with weight about 45 kg were selected and randomly divided into 3 groups: group Ⅰ (control group, without tannin), group Ⅱ (2% tannin) and group Ⅲ (4% tannin), with 5 sheep in each group. The pre-trial period was 14 days and the formal trial period was 60 days. At 6 h after morning feeding, rumen fluid was collected by oral sampler, and then microbial DNA was extracted to measure the number of ruminal bacteria and methanogens by using real-time qPCR and the diversity of ruminal archaea by using the Illumina Miseq PE 300 platform. The results showed as follows: 1) the number of general bacteria, Ruminococcus albus, Butyrivibrio fibrisolvens and Fibrobacter succinogenes of groups Ⅱ and Ⅲ were significantly lower than those of group Ⅰ (P<0.05). Compare with group Ⅰ, the number of Ruminococcus flavefaciens of group Ⅱ was significantly decreased (P<0.05). The number of methanogens and Methaneobrevibacterium of group Ⅲ were significantly lower than those of group Ⅰ (P<0.05). 2) The Ace index of ruminal archaea of groups Ⅱ and Ⅲ was significant lower than that of group Ⅰ (P<0.05). The Shannon index of ruminal archaea of group Ⅲ was significantly lower than that of group Ⅰ (P<0.05). In addition, the Simpson index of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), and Simpson index of group Ⅱ was significantly higher than that of group Ⅰ (P<0.05). In conclusion, 2% and 4% tannin in diets can decrease the number of ruminal general bacteria and methanogens; the community richness and diversity of ruminal archaea are also decreased.

Key words: tannin; sheep; rumen; bacteria; diversity

Cite this article

LI Li , LI Dabiao , WANG Jingyao , XIE Yongfang , LI Zinan . Effects of Different Levels of Tannin in Diets on Rumen Bacteria, Methanogens Number and Rumen Archaea Diversity in Sheep[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2722 -2729 . DOI: 10.3969/j.issn.1006-267x.2020.06.031

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