Effects of Replacement of Fish Meal by Soybean Meal on Growth Performance, Protein Utilization and Intestinal Tissue Morphology of Channa argus

  • ZHANG Xin ,
  • HAN Bei ,
  • HU Juntao ,
  • LIU Lingjun ,
  • CHEN Yueyang ,
  • XU Wenjing ,
  • MIAO Shuyan
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225000, China

Received date: 2019-12-11

  Online published: 2020-06-16

Abstract

This experiment was conducted to investigate the effects of replacement of fish meal by soybean meal on growth performance, protein utilization and intestinal tissue morphology of Channa argus (C. argus), to obtain the suitable replacement proportion of fish meal by soybean meal in the diet of C. argus. A basal diet (group D1) was formulated using 45% fish meal as the main protein source, then different levels of soybean meal were used to replace 20% (group D2), 30% (group D3), 40% (group D4) and 60% (group D5) of fish meal on the basis of the basal diet, to made five isonitrogenous (crude protein content was 44%) and isoenergetic (crude lipid content was 9%) experimental diets. A total of 225 C. argus with the average body weight of (19.07±0.07) g were randomly divided into 5 groups with 3 replicates in each group. Each group had 15 C. argus, which were stored in a 200 L indoor water fiberglass tank, and fed experimental diets for 6 weeks. The results showed that no significant difference in the survival rate (SR) was found among groups (P>0.05). The weight gain rate (WGR), specific growth rate (SGR) and protein efficiency ratio (PER) in groups D4 and D5 were significantly lower than those in group D1 (P<0.05). The feed conversion rate (FCR) in groups D4 and D5 was significantly higher than that in groups D1, D2 and D3 (P<0.05); there were no significant difference in growth performance indices between groups D1 and D2 (P>0.05). Soybean meal replaced different proportion of fish meal had no significant differences in the contents of crude protein and ash in muscle (P>0.05), and the contents of crude lipid and moisture in muscle were not significantly different among groups D1, D2 and D3 (P>0.05), while the moisture content in groups D4 and D5 was significantly lower than that in group D1 (P<0.05), and crude lipid was significantly higher than that in group D3 (P<0.05). The intestinal chymotrypsin activity in groups D4 and D5 had no significant difference (P>0.05), but significantly higher than that in the other 3 groups (P<0.05). The lowest intestinal trypsin and pepsin activities were found in group D5 (P<0.05), and significantly lower than those in groups D1 and D2 (P<0.05). In addition, obvious intestinal damage, including the uneven thickness of intestinal wall, the short and sparse intestinal folds, was found in the C. argus when replacing 30% to 60% fish meal with soybean meal. Taken together, considering the perspective of no effects on the growth performance, protein utilization and intestinal tissue morphology, the replacement proportion of fish meal by soybean meal should not exceed 20% in the C. argus diet which containing 45% fish meal.

Cite this article

ZHANG Xin , HAN Bei , HU Juntao , LIU Lingjun , CHEN Yueyang , XU Wenjing , MIAO Shuyan . Effects of Replacement of Fish Meal by Soybean Meal on Growth Performance, Protein Utilization and Intestinal Tissue Morphology of Channa argus[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2799 -2807 . DOI: 10.3969/j.issn.1006-267x.2020.06.040

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