Effects of Seaweed Polysaccharides on Expression of Tight Junction Protein and Related Genes in Nuclear Transcription Factor-&kappa;B Signaling Pathway in Porcine Epithelial Cells

GUO Xiaobo; ZOU Tiande; YANG Jin; ZENG Yongdi; WANG Zirui; YOU Jinming

doi:10.3969/j.issn.1006-267x.2020.06.042

2020 , Vol. 32 >Issue 6: 2816 - 2823

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.042

Effects of Seaweed Polysaccharides on Expression of Tight Junction Protein and Related Genes in Nuclear Transcription Factor-κB Signaling Pathway in Porcine Epithelial Cells

GUO Xiaobo ,
ZOU Tiande ,
YANG Jin ,
ZENG Yongdi ,
WANG Zirui ,
YOU Jinming

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1. Jiangxi Province Key Innovation Center for Industry-Education Integration of High Quality and Safety Livestock Production, Jiangxi Province Feed Development Engineering Research Center, Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, China;
2. Ganzhou Animal Husbandry Research Institute, Ganzhou 341401, China

Received date: 2020-01-07

Online published: 2020-06-16

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Abstract

This study aimed to evaluate the effects of different concentration of seaweed polysaccharides on expression of tight junction protein and related genes in nuclear transcription factor-κB (NF-κB) signaling pathway in porcine epithelial cells (IPEC-J2 cells). The IPEC-J2 cells were treated with seaweed polysaccharides culture medium with concentrations of 0 (control), 60, 120 and 240 μg/mL, cultured for 24 h, and the effects of seaweed polysaccharides on expression of mechanical barrier and immune barrier related gene and protein in IPEC-J2 cells were analysed. The results showed as follows: 1) compared with the control group, 60 and 120 μg/mL seaweed polysaccharides significantly upregulated the mRNA relative expression levels of occludin and zonula occluden-1 (ZO-1) (P<0.05); but no significant differences were observed in the mRNA relative expression levels of occludin and ZO-1 between control group and 240 μg/mL seaweed polysaccharides group (P>0.05). Compared with control group, 120 and 240 μg/mL seaweed polysaccharides significantly upregulated the mRNA relative expression level of claudin-1 (P<0.05). Compared with control group, 120 and 240 μg/mL seaweed polysaccharides significantly upregulated the protein relative expression levels of claudin-1 and ZO-1 (P<0.05). 2) Compared with control group, 60, 120 and 240 μg/mL seaweed polysaccharides significantly decreased the mRNA relative expression levels of myeloid differentiation protein 88 (MyD88) and p65 (P<0.05), and 240 μg/mL seaweed polysaccharides significantly decreased the mRNA relative expression levels of Toll like receptor-4 (TLR-4) and nuclear transcription factor inhibitor protein α (IκBα) (P<0.05). In conclusion, seaweed polysaccharides can upregulate the mRNA relative expression levels of occludin, claudin-1 and ZO-1 in IPEC-J2 cells, and decrease the expression of NF-κB signaling pathway related gene in IPEC-J2 cells, and have positive effects on improv the barrier function of porcine epithelial cells.

Key words： seaweed polysaccharide; IPEC-J2; intestinal barrier; tight junction protein; NF-κB signaling pathway

Cite this article

GUO Xiaobo , ZOU Tiande , YANG Jin , ZENG Yongdi , WANG Zirui , YOU Jinming . Effects of Seaweed Polysaccharides on Expression of Tight Junction Protein and Related Genes in Nuclear Transcription Factor-κB Signaling Pathway in Porcine Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2816 -2823 . DOI: 10.3969/j.issn.1006-267x.2020.06.042

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