Determination of Mannitol and Lactulose Contents in Goats&rsquo; Plasma by Gas Chromatography-Mass Spectrometry Method

XU Liwei; CHEN Wen; GENG Meimei; ZHANG Liping; HE Zhen; YUAN Hongzhao; PENG Can; LI Chunyong; HE Zhixiong; WANG Jiurong

doi:10.3969/j.issn.1006-267x.2020.06.043

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 6: 2824 - 2830

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.043

Determination of Mannitol and Lactulose Contents in Goats’ Plasma by Gas Chromatography-Mass Spectrometry Method

XU Liwei ,
CHEN Wen ,
GENG Meimei ,
ZHANG Liping ,
HE Zhen ,
YUAN Hongzhao ,
PENG Can ,
LI Chunyong ,
HE Zhixiong ,
WANG Jiurong

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Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2019-12-02

Online published: 2020-06-16

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Abstract

A gas chromatography-mass spectrometry method based on aldononitrile acetate derivatization reaction was developed to determine the goats’ plasma mannitol and lactulose contents. Firstly, the goats’ plasma was treated with two times volume of acetonitrile, and the supernatant was centrifuged. The precipitate was cleaned with one times volume of water, and the supernatant was centrifuged. The two supernatants were combined and dried for aldononitrile acetates derivation. The contents of mannitol and lactulose in derivatives were analyzed by the gas chromatography-mass spectrometry method. The internal standard melibiose, mannitol and lactulose were separated by HP-5 capillary column within 22.75 min. The results showed that the relative standard derivations of retention time and concentration for mannitol and lactulose were less than 0.5% and 5.0%, respectively. Mannitol and lactulose contents showed a good linear relationship at a range of 0.18 to 10.00 μg/mL and 0.23 to 10.00 μg/mL, respectively, and the correlation coefficients of them both more than 0.998. The detection limits of mannitol and lactulose were 0.052 and 0.071 μg/mL, and the recovery of standard addition were 71.3% to 72.5% and 89.8% to 110.0%, respectively. In conclusion, the gas chromatography-mass spectrometry method developed in this experiment to determine the goats’ plasma mannitol and lactulose contents has strong selectivity and high sensitivity, which can be used for the intestinal permeability evaluation in animals.

Key words： lactulose; mannitol; internal standard method; aldononitrile acetates derivation; gas chromatography-mass spectrometry

Cite this article

XU Liwei , CHEN Wen , GENG Meimei , ZHANG Liping , HE Zhen , YUAN Hongzhao , PENG Can , LI Chunyong , HE Zhixiong , WANG Jiurong . Determination of Mannitol and Lactulose Contents in Goats’ Plasma by Gas Chromatography-Mass Spectrometry Method[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2824 -2830 . DOI: 10.3969/j.issn.1006-267x.2020.06.043

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