Effects of Bacillus amyloliquefaciens fsznc-06 Isolated from Goats on Growth and Immune Function of Mice

ZHANG Nanchi; LI Juan; WANG Li; WEI Yong

doi:10.3969/j.issn.1006-267x.2020.06.046

Chinese Journal of Animal Nutrition >

2020 , Vol. 32 >Issue 6: 2861 - 2868

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.046

Effects of Bacillus amyloliquefaciens fsznc-06 Isolated from Goats on Growth and Immune Function of Mice

ZHANG Nanchi ,
LI Juan ,
WANG Li ,
WEI Yong

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1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China;
2. Animal Genetics and Breeding Key Laboratory of Sichuan Province, Animal Science Academy of Sichuan Province, Chengdu 610066, China

Received date: 2019-12-08

Online published: 2020-06-16

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Abstract

This experiment was conducted to investigate the effects of Bacillus amyloliquefaciens fsznc-06 isolated from goats on growth and immune function of mice. Thirty-six healthy mice were randomly divided into 3 groups with 3 replicates per group and 4 mice per replicate. Mice in low dose group (JD1 group) and high dose group (JD2 group) were given 1×10⁸ and 1×10¹⁰ CFU/mL Bacillus amyloliquefaciens fsznc-06 isolated from goats by gavage once every 2 days, and the gavage volume was 0.3 mL per mouse; mice in the control group (DZ group) were given 0.9% normal saline by gavage once every 2 days, and the gavage volume was 0.3 mL per mouse. The experiment lasted for 28 days. The growth performance, serum biochemical index, expression levels of growth and immune related genes and intestinal flora diversity of mice were measured. The results showed as follows: 1) compared with the DZ group, the body weight gain on days 7, 14, 21 and 28 of JD1 group was significantly increased (P<0.05 or P<0.01), and the body weight gain on days 7 and 14 of JD2 group was significantly increased (P<0.05 or P<0.01). Compared with the DZ group, the feed to gain ratio on days 1 to 7, days 8 to 14, days 15 to 21 and days 22 to 28 of JD1 group was significantly decreased (P<0.05 or P<0.01), and the feed to gain ratio on days 1 to 7, days 15 to 21 and days 22 to 28 of JD2 group was significantly decreased (P<0.05 or P<0.01). 2) Compared with the DZ group, the indexes of heart and small intestine of JD1 group and indexes of heart, spleen, thymus and large intestine of JD2 group were significantly increased (P<0.05 or P<0.01), the activities of total superoxide dismutase (T-SOD), alkaline phosphatase (AKP) in serum of JD1 group and serum AKP activity of JD2 group were significantly increased (P<0.05 or P<0.01). 3) Compared with the DZ group, the spleen insulin-like growth factor-1 (IGF-1) gene expression level of JD1 group and expression levels of IGF-1 and interferon alpha 11 (IFNα11) in spleen of JD2 group were significantly increased (P<0.05 or P<0.01), and the expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) in spleen of JD1 group and expression levels of IL-1β, interleukin-6 (IL-6), nitric oxide synthase (iNOS) and TNF-α in spleen of JD2 group were significantly decreased (P<0.05 or P<0.01). 4) Compared with the DZ group, the intestinal flora diversity and relative abundance of Bifidobacteria of JD1 and JD2 groups were increased, and the relative abundance of dominant species was also increased. In conclusion, Bacillus amyloliquefaciens fsznc-06 isolated from goats can promote the growth and improve the immune funtion of mice, and JD2 group has a relatively better effect.

Key words： Bacillus amyloliquefaciens; mice; growth; immune; intestinal flora

Cite this article

ZHANG Nanchi , LI Juan , WANG Li , WEI Yong . Effects of Bacillus amyloliquefaciens fsznc-06 Isolated from Goats on Growth and Immune Function of Mice[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2861 -2868 . DOI: 10.3969/j.issn.1006-267x.2020.06.046

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