Effects of Glucose on Cell Proliferation, Glucose Uptake and Expression of Casein Synthesis-Related Genes in Mammary Epithelial Cells of Dairy Cows

LI Zinan; LI Dabiao; XING Yuanyuan; JIN Yaya; MU Xijia; CAO Yue

doi:10.3969/j.issn.1006-267x.2020.06.050

2020 , Vol. 32 >Issue 6: 2896 - 2903

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.050

Effects of Glucose on Cell Proliferation, Glucose Uptake and Expression of Casein Synthesis-Related Genes in Mammary Epithelial Cells of Dairy Cows

LI Zinan ,
LI Dabiao ,
XING Yuanyuan ,
JIN Yaya ,
MU Xijia ,
CAO Yue

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College of Animal Science, Inner Mongolia Agriculture University, Hohhot 010018, China

Received date: 2019-12-11

Online published: 2020-06-16

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Abstract

The aim of this study was to investigate the effects of different concentrations of glucose on the proliferation, glucose uptake and the expression of genes related with casein synthesis in bovine mammary epithelial cells (BMECs). BMECs from Chinese Holstein cows were culture and purification. A single factor randomized trial design was applied. Different concentrations [0 (control), 5, 10 and 20 mmol/L] of glucose were added in culture medium. Each group had 3 replicates. The results showed as follows: 1) compared with the control group, adding glucose significantly increased the relative proliferation rate of BMCEs (P<0.05). 2) Compared with the control group, the glucose related uptake rate of BMECs in 10 mmol/L group significantly promoted(P<0.05). 3) Compared with the control group, the gene expression of glucose transporter 1, glucose transporter 8 and glucose transporter 12 in 10 to 20 mmol/L groups significantly up-regulated (P<0.05). 4) Compared with the group, the αs1-casein, κ-casein, rapamycin target molecule, eukaryotic translation initiation factor 4E binding protein, eukaryotic initiation factor 4E and ribosomal p70s6 gene expression in 10 mmol/L group significantly up-regulated (P<0.05). Combined with the above results, the addition of 10 to 20 mmol/L glucose can promote the uptake of glucose in BMECs, and up-regulate the expression of genes related with casein synthesis.

Key words： bovine mammary epithelial cells; glucose; glucose transporter; casein

Cite this article

LI Zinan , LI Dabiao , XING Yuanyuan , JIN Yaya , MU Xijia , CAO Yue . Effects of Glucose on Cell Proliferation, Glucose Uptake and Expression of Casein Synthesis-Related Genes in Mammary Epithelial Cells of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2896 -2903 . DOI: 10.3969/j.issn.1006-267x.2020.06.050

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