Effects of Exogenous Cellulose on in Vitro Rumen Fermentation Characteristics of Weaner Lambs

  • MENG Fang ,
  • LIU Lishan ,
  • LANG Xia ,
  • WANG Cailian ,
  • WU Jianping ,
  • ZHOU Rui
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  • 1. Key Laboratory for Sheep, Goat and Cattle Germplasm and Straw Feed in Gansu Province, Institute of Animal and Pasture Science and Green Agricultural, Gansu Academy of Agricultural Science, Lanzhou 730070, China;
    2. Institute of Rural Development of Northwest Normal University, Lanzhou 730070, China;
    3. Life Science and Engineering College of Northwest Minzu University, Lanzhou 730030, China

Received date: 2019-11-03

  Online published: 2020-06-16

Abstract

This study aimed to observe the effects of exogenous cellulose on in vitro rumen fermentation parameters and digestive enzyme activities of weaner lambs. A single-factor experimental design was used in this study. Exogenous cellulose was supplemented in concentrate at the levels of 0 (CON group, as control), 280 000 (T1 group), 300 000 (T2 group) and 320 000 U/kg (T3 group), respectively. According to the forage-concentrate ratio of 36.45:63.55, basal diets were formulated and used as fermentation substrates, and the dry matter degradability (DMD), fermentation parameters and digestive enzyme activities were measured after fermented different time (0, 1, 2, 4, 8, 12, 24, 48 and 72 h). The results showed as follows: compared with the CON group, the DMD, microprotein (MCP) concentration of T2 group was significantly increased (P<0.05), and the ammonia nitrogen (NH3-N) concentration was significantly decreased (P<0.05), but the treatment and fermentation time had no significant interaction on above indices (P>0.05). With the fermentation time extension, the DMD was gradually increased, the change tendency of NH3-N and MCP concentrations was opposite, NH3-N concentration reached peak values at 2 and 8 h after fermentation, but MCP concentration reached valley values at 2 and 8 h after fermentation. Treatment and fermentation time had no significant effects on each volatile fatty acid (VFA) concentration and acetate/propionate (A/P), but total VFA concentration of T2 group was significantly higher than that of CON group (P<0.05), moreover, the treatment and fermentation time had no significant interaction on above indices (P>0.05). The α-amylase and cellulose activities of T2 group were significantly higher than those of CON group (P<0.05), and the interaction of treatment and fermentation time on α-amylase activity was significant (P<0.05). With the fermentation time extension, the α-amylase activity of each group showed a fluctuation change tendency, it reached peak values at 4 and 12 h and valley values at 2, 8 and 24 h after fermentation. The protease activity was gradually decreased at 0 to 4 h after fermentation, and then it fluctuated. For cellulose activity, T3 group was lower than other groups at 4 h after fermentation, T2 group was higher than other groups at 8 h after fermentation, T1 group was higher than other groups at 24 h after fermentation. In summary, supplementation of 300 000 U/kg exogenous cellulose in the concentrate can improve the in vitro DMD of diet, enhance the synthesis of MCP, increase the utilization of NH3-N, and increase the activities of α-amylase and cellulose, but do not affect the protease activity of weaner lambs.

Cite this article

MENG Fang , LIU Lishan , LANG Xia , WANG Cailian , WU Jianping , ZHOU Rui . Effects of Exogenous Cellulose on in Vitro Rumen Fermentation Characteristics of Weaner Lambs[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2911 -2920 . DOI: 10.3969/j.issn.1006-267x.2020.06.052

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