Effects of Flammulina velutipes Medium Residue on Growth Performance, Intestinal Tissue Structure and Serum Biochemical, Immune and Antioxidant Indexes of Grass Carp

ZHU Bo; ZHONG Lei; ZHAO Qinghai; DAI Zhenyan; CHEN Kaijian; HU Yi

doi:10.3969/j.issn.1006-267x.2020.06.053

2020 , Vol. 32 >Issue 6: 2921 - 2929

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.053

Effects of Flammulina velutipes Medium Residue on Growth Performance, Intestinal Tissue Structure and Serum Biochemical, Immune and Antioxidant Indexes of Grass Carp

ZHU Bo ,
ZHONG Lei ,
ZHAO Qinghai ,
DAI Zhenyan ,
CHEN Kaijian ,
HU Yi

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1. College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;
2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China

Received date: 2019-12-03

Online published: 2020-06-16

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Abstract

This experiment was conducted to study the effects of Flammulina velutipes medium residue on growth performance, intestinal tissue structure and serum biochemical, immune and antioxidant indexes of grass carp. A total of 480 grass carp with initial body weight of (20.02±0.03) g were randomly divided into 4 groups with 3 replicates per group and 40 fish per replicate. Fish in the control group were fed a basal diet, and others in the experimental groups were fed equal nitrogen and equal lipid experimental diets which Flammulina velutipes medium residue supplemental levels were 5% (group Ⅰ), 10% (group Ⅱ) and 15% (group Ⅲ), respectively. The experiment lasted for 56 days. The results showed as follows: 1) the weight gain rate of grass carp in group Ⅰ was significantly higher than that in control group and groups Ⅱ and Ⅲ (P<0.05), and there were no significant differences in the feed conversion rate, survival rate, condition factor, hepatosmatic index and viserosomatic index of grass carp among all groups (P>0.05). 2) The activities of amylase and lipase in intestine in group Ⅰ were significantly higher than those in group Ⅲ (P>0.05), and the intestinal trypsin activity in group Ⅱ was significantly higher than that in control group and group Ⅲ (P>0.05). 3) The intestinal villus height and goblet cell number in group Ⅰ were significantly higher than those in the control group (P<0.05), and the intestinal muscle layer thickness in groups Ⅱ and Ⅲ was significantly higher than that in the control group (P<0.05). 4) Compared with the control group, the serum glutamic oxaloacetylase activity in group Ⅰ was significantly decreased (P<0.05), the serum complement 4 content in group Ⅰ was significantly increased (P<0.05), the complement 3, immunoglobulin M contents and alkaline phosphatase activity in serum in groups Ⅱ and Ⅲ were significantly decreased (P<0.05), the serum catalase activity in groups Ⅱ and Ⅲ was significantly decreased (P<0.05), the serum antisuperoxideanion free radical activity in group Ⅲ was significantly decreased (P<0.05), and the serum malondialdehyde content in group Ⅲ was significantly increased (P<0.05). In conclusion, dietary supplemented 5% Flammulina mushroom medium residue can improve the growth performance of grass carp, improve intestinal tissue structure and digestive enzyme activity. It is suggested that the suitable dietary Flammulina mushroom medium residue supplemental level is less than 10% according to the growth performance, intestinal tissue structure and serum biochemical, immune and antioxidant indexes of grass carp.

Key words： Flammulina mushroom medium residue; grass carp; serum biochemical; immune; intestinal tissue structure

Cite this article

ZHU Bo , ZHONG Lei , ZHAO Qinghai , DAI Zhenyan , CHEN Kaijian , HU Yi . Effects of Flammulina velutipes Medium Residue on Growth Performance, Intestinal Tissue Structure and Serum Biochemical, Immune and Antioxidant Indexes of Grass Carp[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2921 -2929 . DOI: 10.3969/j.issn.1006-267x.2020.06.053

References

[1] CHOU W T,SHEIH I C,FANG T J.The applications of polysaccharides from various mushroom wastes as prebiotics in different systems[J].Journal of Food Science,2013,78(7):M1041-M1048.
[2] ZHANG T T,YE J F,XUE C H,et al.Structural characteristics and bioactive properties of a novel polysaccharide from Flammulina velutipes[J].Carbohydrate Polymers,2018,197:147-156.
[3] 范文丽,李天来,代洋,等.杏鲍菇、香菇、金针菇、蛹虫草、滑菇、平菇菌糠营养分析评价[J].沈阳农业大学学报,2013,44(5):673-677.
[4] 赵晓丽,刘学铭,陈智毅,等.金针菇菌糠不同部位营养成分比较[J].食用菌学报,2012,19(4):21-24.
[5] 王利民,王文志,孙海涛,等.金针菇菌渣代替麸皮对肉兔生产性能的影响[J].山东农业科学,2014,46(5):113-114,117.
[6] 孟梅娟,高立鹏,白云峰,等.金针菇菌渣对山羊饲粮养分表观消化率及氮平衡的影响[J].动物营养学报,2016,28(9):2953-2961.
[7] DASGUPTA M.Adaptation of the alimentary tract to feeding habits in the weed eating fish (grass carp) Ctenopharyngodon idella (Val.)[J].Journal of Crop and Weed,2009,5(1):197-202.
[8] HAO Y T,WU S G,JAKOVLI? I,et al.Impacts of diet on hindgut microbiota and short-chain fatty acids in grass carp (Ctenopharyngodon idellus)[J].Aquaculture Research,2017,48(11):5595-5605.

[9] 吴桐强,钟蕾,刘庄鹏,等.谷氨酰胺二肽对草鱼幼鱼生长、血清生化、免疫指标及肠道组织结构的影响[J].动物营养学报,2019,31(8):3682-3689.
[10] 黄立春,韩书煜,李凌波.姬菇菌糠配合商品饲料养殖草鱼的试验[J].河北渔业,2018(4):19-20.
[11] 庞思成.使用菌糠饲养罗非鱼技术[J].淡水渔业,1993,23(5):32-34.
[12] 晁洪雨,李福昌.日料ADF水平对生长肉兔氮平衡、免疫及消化酶活性的影响[J].中国饲料,2007(15):18-20,23.
[13] HSU J C,CHEN L I,YU B.Effects of levels of crude fiber on growth performances and intestinal carbohydrases of domestic goslings[J].Asian Australasian Journal of Animal Sciences,2000,13(10):1450-1454.

[14] SANTOS E A D,LUI J F,SCAPINELLO C.Effect of acid detergent fiber levels on digestibility coefficients and performance of growing rabbits[J].Acta Scientiarum Animal Sciences,2004,26(1):79-86.
[15] 唐倩,杨小婷,李吕木,等.日粮粗纤维水平对圩猪生长性能、肉品质及血液生化指标的影响[J].西北农林科技大学学报(自然科学版),2016,43(8):10-18.
[16] 田芊芊,胡毅,毛盼,等.低鱼粉饲料中添加牛磺酸对青鱼幼鱼生长、肠道修复及抗急性拥挤胁迫的影响[J].水产学报,2016,40(9):1330-1339.
[17] LIU Y,ZHAO J,LIAO D H,et al.Low-residue diet fed to rabbits induces histomorphological and biomechanical remodeling of small intestine[J].Neurogastroenterology & Motility,2016,29(4):e12983.
[18] 陈晨,黄峰,舒秋艳,等.共轭亚油酸对草鱼生长、肌肉成分、谷草转氨酶及谷丙转氨酶活性的影响[J].水生生物学报,2010,34(3):647-651.
[19] 孟梅娟,白云峰,高立鹏,等.金针菇菌渣日粮对山羊养分表观消化率及血清生化指标的影响[J].家畜生态学报,2017,38(5):31-37.
[20] HOSOMI R,FUKUNAGA K,ARAI H,et al.Fish protein decreases serum cholesterol in rats by inhibition of cholesterol and bile acid absorption[J].Journal of Food Science,2011,76(4):H116-H121.
[21] LUO L,SCHOMAKER S,HOULE C,et al.Evaluation of serum bile acid profiles as biomarkers of liver injury in rodents[J].Toxicological Sciences,2014,137(1):12-25.

[22] 徐翱.饲料中添加不同比例冰鲜鱼、纤维素对鲟亲鱼血清生化指标及繁殖性能的影响[D].硕士学位论文.上海:上海海洋大学,2014.
[23] BUNTING L D,BOLING J A,MACKOWN C T,et al.Effect of dietary protein level on nitrogen metabolism in lambs:studies using ¹⁵N-nitrogen[J].Journal of Animal Science,1987,64(3):855-867.

[24] KASHINA S,VILLAVICENCIO L L F,ZAINA S,et al.Activity of extracts from submerged cultured mycelium of winter mushroom,Flammulina velutipes (agaricomycetes),on the immune system in vitro[J].International Journal of Medicinal Mushrooms,2016,18(1):49-57.

[25] 姜秀梅,徐小磊,殷一民,等.金针菇多糖对衰老小鼠大脑抗氧化能力的影响[J].中国老年学杂志,2016,36(23):5797-5799.
[26] ZHANG T T,YE J F,XUE C H,et al.Structural characteristics and bioactive properties of a novel polysaccharide from Flammulina velutipes[J].Carbohydrate Polymers,2018,197:174-156.
[27] 崔嘉,郭佳伟,忻龙祚,等.金针菇菌糠饲用价值评价[J].饲料研究,2017(9):37-41.
[28] YANG H S,WU F,LONG L N,et al.Effects of yeast products on the intestinal morphology,barrier function,cytokine expression,and antioxidant system of weaned piglets[J].Journal of Zhejiang University-SCIENCE B,2016,17(10):752-762.

[29] 解伟,郭冉,夏辉,等.黄曲霉毒素B₁对凡纳滨对虾幼虾肝胰腺抗氧化酶的损伤机制[J].水产学报,2017,41(3):131-138.
[30] 刘高科,汪正清.补体在肠缺血再灌注损伤中的作用及机制[J].免疫学杂志,2010,26(3):271-274.
[31] 昝琦,刘欣,逄越,等.补体C3结构与功能研究进展[J].中国免疫学杂志,2014,30(4):122-126.

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