Histological Study on Morphology, Growth and Digestive Tract Development of Loach (Misgurnus anguillicaudatus) at Seedling Stage

GAO Miao; ZHANG Wenping; ZHOU Qiubai; ZHANG Zhengzhou; WANG Zirui; WANG Ying

doi:10.3969/j.issn.1006-267x.2020.06.054

2020 , Vol. 32 >Issue 6: 2930 - 2940

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.06.054

Histological Study on Morphology, Growth and Digestive Tract Development of Loach (Misgurnus anguillicaudatus) at Seedling Stage

GAO Miao ,
ZHANG Wenping ,
ZHOU Qiubai ,
ZHANG Zhengzhou ,
WANG Zirui ,
WANG Ying

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College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China

Received date: 2019-12-11

Online published: 2020-06-16

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Abstract

In this paper, morphological and histological methods were used to study the morphology, growth and digestive tract tissue development of larvae, juvenile and juvenile loach (1 to 70 days of age). A total of 1 500 larvae hatched from the same pair of loach were selected and divided into 3 replicates, each with 500 larvae. The feeding period was 70 d. Results showed as follows: 1)the relationship between body length (L) and body weight (W) of loach was as follows: W=0.008 8L^{3.019 8}(R²=0.994 0). 2) The newly hatched loach had a transparent body color, and had outer gill and yolk sac. The digestive tract was a closed straight canal, which was composed of monolayer columnar epithelial cells. At 3 days of age, the yolk sac of larvae loach nearly disappeared. Food residue appeared in the digestive tract, and the anus communicated with the external environment. The outer gill of larvae at 5 days of age appeared degeneration, the gill periosteum extended backward, and the inner gill filaments appeared. Folds of intestinal mucosa increased significantly, and clear striated border could be seen in the anterior intestine and middle intestine. From 15 to 25 days of age, the outer gill disappeared completely, the tissue structure of the anterior intestine, middle intestine and posterior intestine was significantly different. The muscular wall of the anterior intestine became thicker and the mucosal folds deepened, and the mucosal folds of the middle intestine were shorter than those of the anterior intestine. The striated border of the anterior intestine and middle intestine was obvious. The folds of the posterior intestine mucosa were smooth. The mucosal epithelium was thin and transparent, and the submucosa was rich in microvascular network. In conclusion, loach belongs to constant growth type. At 3 days of age, juvenile loach take nourishment from endogenous nutrition to exogenous nutrition. Intestinal digestion and gas breathing function are not complete from 3 to 15 days of age juvenile loach. While intestinal structure development from 15 to 25 days of age juvenile loach is basically completed, and the digestive function has tended to be completed. Posterior intestine has a more complete gas breathing function. Combined with intestinal development of loach to provide palatability bait. Making good use of the dual functions of gill and intestinal gas breathing is beneficial to improve the survival rate of juvenile loach.

Key words： loach (Misgurnus anguillicaudatus); juvenile loach growth; outer gill; digestive tract structure; intestinal gas breath

Cite this article

GAO Miao , ZHANG Wenping , ZHOU Qiubai , ZHANG Zhengzhou , WANG Zirui , WANG Ying . Histological Study on Morphology, Growth and Digestive Tract Development of Loach (Misgurnus anguillicaudatus) at Seedling Stage[J]. Chinese Journal of Animal Nutrition, 2020 , 32(6) : 2930 -2940 . DOI: 10.3969/j.issn.1006-267x.2020.06.054

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