Effects of Lactoferrin on Reproductive Performance of Dahe Black Sows and Iron Nutrition, Blood Cell Parameters, Immune Indexes and Porcine &beta;-Defensin Gene Expression of Sows and Piglets

CHE Litao; LI Cenxi; XU Nana; ZHANG Chunyong; LIU Shuiling; AN Qingcong; GUO Rongfu

doi:10.3969/j.issn.1006-267x.2020.07.018

2020 , Vol. 32 >Issue 7: 3089 - 3098

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.018

Effects of Lactoferrin on Reproductive Performance of Dahe Black Sows and Iron Nutrition, Blood Cell Parameters, Immune Indexes and Porcine β-Defensin Gene Expression of Sows and Piglets

CHE Litao ,
LI Cenxi ,
XU Nana ,
ZHANG Chunyong ,
LIU Shuiling ,
AN Qingcong ,
GUO Rongfu

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Key Laboratory of Animal Nutrition and Feed Science of Yunnan Province, Yunnan Agricultural University, Kunming 650201, China

Received date: 2020-01-22

Online published: 2020-07-15

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Abstract

This experiment was conducted to investigate the effects of effects of lactoferrin(LF) on reproductive performance of Dahe black sow and iron nutrition, blood cell parameters, immune indexes and porcine β-defensin (pBD) gene expression of sows and piglets. Sixty multiparity (3 to 4 fetuses) Dahe black sows with similar litter size and body weight were randomly divided into 5 groups with 12 replicates in each group and 1 sow in each replicate. From the 80th day of gestation to the delivery, sows the control group were fed a basal diet, sows in the LF1, LF2 and LF3 groups were fed the basal diets supplemented with 100, 200 and 300 mg/kg LF, respectively, and sows in the iron glycine chelate (Fe-Gly) were fed the basal diet supplemented with 500 mg/kg Fe-Gly. The reproductive performance of sows was examined, the red blood cell count (RBC) and hemoglobin (HGB) content in blood, serum immune indexes, sow serum and milk iron content were measured, and the expression level of pBD gene in liver, spleen and duodenal of piglets were detected. The results showed as follows:1) compared with the control group, the weight at birth and litter weight at birth of piglets of LF2 group were significantly increased (P<0.05), the litter weight at birth of piglets of LF1 and LF3 groups was significantly increased (P<0.05). 2) Compared with the control group, the blood RBC and HGB content of sows of LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05), and the blood RBC and HGB content of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly increased (P<0.05). 3) Compared with the control group, the contents of iron in serum and milk of sows of LF1, LF2 and LF3 groups were significantly increased (P<0.05). 4) Compared with the control group, the serum lactate dehydrogenase (LDH) activity of piglets of LF1, LF2 and LF3 groups and Fe-Gly groups was significantly decreased (P<0.05), and the serum nitric oxide synthase (NOS) activity was significantly increased (P<0.05); the serum immunoglobulin G (IgG) content and lysozyme (LSZ) activity of piglets of LF1 and LF2 groups were significantly increased (P<0.05). 5) Compared with the control group, the expression levels of pBD-1 gene in liver, spleen and duodenum, pBD-2 gene in liver and spleen and pBD-3 gene in spleen of piglets of LF2 group were significantly increased (P<0.05); the expression levels of pBD-1 and pBD-2 genes in liver of piglets of LF1 group and pBD-1 in liver and spleen of piglets of LF3 group were significantly increased (P<0.05); and the expression levels of pBD-2 gene in duodenum and pBD-3 gene in liver and duodenum of piglets of LF1, LF2, LF3 and Fe-Gly groups were significantly decreased (P<0.05). In conclusion, dietary adding appropriate dose (200 mg/kg) LF of Dahe black sow in the late pregnancy can increase the weight at birth and litter weight at birth of piglets, improve the iron nutrition and serum immune indexes of piglets, increase the expression levels of pBD-1 gene in liver, spleen and duodenum, which is beneficial to improve the disease resistance of piglets.

Key words： lactoferrin; Dahe black sows; reproductive performance; hemoglobin; porcine β-defensin

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CHE Litao , LI Cenxi , XU Nana , ZHANG Chunyong , LIU Shuiling , AN Qingcong , GUO Rongfu . Effects of Lactoferrin on Reproductive Performance of Dahe Black Sows and Iron Nutrition, Blood Cell Parameters, Immune Indexes and Porcine β-Defensin Gene Expression of Sows and Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3089 -3098 . DOI: 10.3969/j.issn.1006-267x.2020.07.018

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