Effects of Mannan Oligosaccharides and Complex Probiotics on Growth Performance and Intestinal Morphology, Volatile Fatty Acid Contents and Microbial Structure of Weaned Piglets

ZHONG Xiaoxia; HUANG Jian; LIU Zhiyun; ZHOU Xiaorong; HUANG Jinxiu

doi:10.3969/j.issn.1006-267x.2020.07.019

2020 , Vol. 32 >Issue 7: 3099 - 3108

DOI: https://doi.org/10.3969/j.issn.1006-267x.2020.07.019

Effects of Mannan Oligosaccharides and Complex Probiotics on Growth Performance and Intestinal Morphology, Volatile Fatty Acid Contents and Microbial Structure of Weaned Piglets

ZHONG Xiaoxia ,
HUANG Jian ,
LIU Zhiyun ,
ZHOU Xiaorong ,
HUANG Jinxiu

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1. Chongqing Academy of Animal Sciences, Chongqing 402460, China;
2. Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing 402460, China

Received date: 2020-01-13

Online published: 2020-07-15

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Abstract

This experiment was conducted to study the effects of dietary mannan oligosaccharides (MOS) and complex probiotics on growth performance and intestinal morphology, volatile fatty acid contents and microbial structure of weaned piglets. A total of 144 Duroc×Landrace×Yorkshire weaned piglets aged from 28 to 30 days with body weight of (8.50±0.11) kg were selected and randomly divided into 4 groups with 6 replicates in each group and 6 pigs in each replicate. Group Ⅰ was the control group, fed a basal diet; group Ⅱ was a MOS group, fed the basal diet+0.1% MOS; group Ⅲ was a complex probiotics group, fed the basal diet+0.1% complex probiotics; group Ⅳ was a MOS and complex probiotics combined group, fed the basal diet+0.1% MOS+0.1% compound probiotic. The pre-experimental period lasted for 5 days, and the experimental period lasted for 28 days. The results showed as follows:1) compared with group Ⅰ, the average daily feed intake of groups Ⅱ and Ⅳ was significantly increased (P<0.05), and the diarrhea rate of group Ⅳ was significantly decreased (P<0.05). 2) Compared with group Ⅰ, the villus height, crypt depth and villus height/crypt depth in jejunum and ileum of groups Ⅱ, Ⅲ and Ⅳ had no significant difference (P>0.05). 3) Compared with group Ⅰ, the contents of butyric acid and total volatile fatty acid in cecum contents of groups Ⅲ and Ⅳ showed an increasing trend (P=0.10); the colon butyric acid content of groups Ⅲ and Ⅳ increased by 59.67% and 60.84% (P<0.05), respectively, and the total volatile fatty acid content showed an increasing trend (P=0.06). 4) Compared with group Ⅰ, the Shannon index in cecum contents of group Ⅱ was significantly decreased (P<0.05), and the Simpson index was significantly increased (P<0.05). At the phylum level, the main dominant floras in cecum and colon contents were Firmicutes, Proteobacteria, Bacteroidetes and Tenericutes. At the genus level, the main dominant floras in cecum contents were Lactobacillus, Clostridium_sensu_stricto_1, Subdoligranulum, Lachnospira and Roseburia, and the main dominant floras in colon contents were Lactobacillus, uncultured_bacterium_f_Bacteroidales_S24-7_group, Clostridium_sensu_stricto_1 and Roseburia. In summary, dietary 0.1% MOS improve the average daily feed intake and hindgut microbial structure of weaned piglets; dietary 0.1% complex probiotics have a positive effect on the hindgut volatile fatty acid contents, especially butyric acid; MOS and complex probiotics combined use have a positive effect on improve the average daily feed intake, reduce diarrhea rate, improve volatile fatty acid contents and improve hindgut microbial structure of weaned piglets.

Key words： mannan oligosaccharides; complex probiotics; weaned piglets; growth performance; microbial structure

Cite this article

ZHONG Xiaoxia , HUANG Jian , LIU Zhiyun , ZHOU Xiaorong , HUANG Jinxiu . Effects of Mannan Oligosaccharides and Complex Probiotics on Growth Performance and Intestinal Morphology, Volatile Fatty Acid Contents and Microbial Structure of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2020 , 32(7) : 3099 -3108 . DOI: 10.3969/j.issn.1006-267x.2020.07.019

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